A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Lund, Bjarte A ^a   Leiros, Hanna Kirsti Schrøder ^a   Bjerga, Gro Elin Kjæreng ^a,b  

^a UNIVERSITY OF TROMSØ   (Norway)

^b UNIVERSITY OF BERGEN   (Norway)

Author keywords

Counterselection; Exponential megapriming PCR; High throughput; Parallel cloning; Recombinant DNA technology; Restriction free cloning

Indexed keywords

BETA LACTAMASE; CYTOTOXIN; DNA FRAGMENT; GENOMIC DNA; MEROPENEM; RECOMBINANT DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; BACTERIAL STRAIN; BETA LACTAM HYDROLASE GENE; CLONING VECTOR; COUPLED CELL DIVISION B GENE; DIMERIZATION; DNA TEMPLATE; ESCHERICHIA COLI; EXPONENTIAL MEGAPRIMING POLYMERASE CHAIN REACTION; EXPRESSION VECTOR; GC RICH SEQUENCE; GENE; GENE INSERTION; GENE REPLACEMENT; GENETIC PROCEDURES; HETEROLOGOUS EXPRESSION; HIGH THROUGHPUT SCREENING; MICHAELIS MENTEN KINETICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; RECOMBINANT GENE; RESTRICTION FREE CLONING; SITE DIRECTED MUTAGENESIS;

BETA-LACTAMASES; CLONING, MOLECULAR; CYTOTOXINS; ESCHERICHIA COLI; GENETIC VECTORS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC;

EID: 84899145860     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-13-38     Document Type: Article

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