Positive selection of recombinant DNA by CcdB

BioTechniques

Volumn 21, Issue 2, 1996, Pages 320-323

  Bernard, Philippe ^a,b  

^a Institute of Biology   (France)

^b BioRix   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; GENE PRODUCT; RECOMBINANT DNA;

CLONING VECTOR; CYTOTOXICITY; DNA RECOMBINATION; GENE MUTATION; MOLECULAR CLONING; PLASMID; REVIEW;

EID: 0029741707     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212pf01     Document Type: Review

References (19)

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2. Bernard, P. 1995. New ccdB positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers. Gene 162:159-160.
3. Bernard, P. and M. Couturier. 1992. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J. Mol. Biol. 226:735-745.
4. Bernard, P. and P. Gabant, inventors; Université Libre de Bruxelles, assignee; Cloning and/or sequencing vector. PCT patent PCT/BE93/00051 and Belgian patent 09200696.
5. Bernard, P., P. Gabant, E.M. Bahassi and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
6. Bernard, P., K. Kézdy, L. Van Melderen, J. Steyaert, L. Wyns, M.L. Pato, N.P. Higgins and M. Couturier. 1993. The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase. J. Mol. Biol. 234:534-541.
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