Restriction site-free insertion of PCR products directionally into vectors

BioTechniques

Volumn 28, Issue 3, 2000, Pages 498-505

  Chen, Guo Jun ^a   Qiu, N ^a   Karrer, C ^a   Caspers, P ^a   Page, M G P ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; CLONING; DNA INTEGRATION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE INSERTION; MUTAGENESIS; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 0034099072     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283st08     Document Type: Article

References (13)

1. Aslanidis, C. and P.J. de Jong. 1990. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18:6069-6074.
2. Bhat, G.J., M.J. Lodes, P.J. Myler and K.D. Stuart. 1991. A simple method for cloning blunt ended DNA fragments. Nucleic Acids Res. 19:398.
3. Haun, R.S., I.M. Serventi and J. Moss. 1992. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13:515-518.
4. Horton, R.M., S.N. Ho, J.K. Pullen, H.D. Hunt, Z. Cai and L.R. Pease. 1993. Gene splicing by overlap extension. Methods Enzymol. 217:270-279.
5. Karn, J., S. Brenner, L. Barnett and G. Cesareni. 1980. Novel bacteriophage lambda cloning vector. Proc. Natl. Acad. Sci. USA 77:5172-5176.
6. Kunkel, T.H., J.D. Roberts and R.A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:367-382.
7. Ling, M.M. and B.H. Robinson. 1997. Approaches to DNA mutagenesis: an overview. Anal. Biochem. 254:157-178.
8. Liu, Z.G. and L.M. Schwartz. 1992. An efficient method for blunt-end ligation of PCR products. BioTechniques 12:28.
9. Marchuk, D., M. Drumm, A. Saulino and F.S. Collins. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
10. Scharf, S.J., G.T. Horn and H.A. Erlich. 1986. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076-1078.
11. Stratagene. QuikChange™ Site-Directed Mutagenesis Protocol and references therein. Stratagene, La Jolla, CA, USA.
12. Stueber, D., H. Matile and G. Garotta. 1990. System for high-level production in E. coli and rapid purification of recombinant proteins: application to epitope mapping, preparation of antibodies and structure-function analysis. p. 121-152. In Lefkovits, I. and B. Pernis (Eds.), Immunological Methods, Vol. IV, Academic Press, Orlando.
13. The NEB Transcript. 1998, vol. 9 No. 1, page 7, GATC: DpnI, DpnII, MboI or Sau3A I. New England BioLabs® Inc., Beverly, MA.