Reaction mechanism of N -acetylneuraminic acid lyase revealed by a combination of crystallography, QM/MM simulation, and mutagenesis

ACS Chemical Biology

Volumn 9, Issue 4, 2014, Pages 1025-1032

  Daniels, Adam D ^a   Campeotto, Ivan ^a,c   Van Der Kamp, Marc W ^b   Bolt, Amanda H ^a   Trinh, Chi H ^a   Phillips, Simon E V ^a,d   Pearson, Arwen R ^a   Nelson, Adam ^a   Mulholland, Adrian J ^b   Berry, Alan ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d RUTHERFORD APPLETON LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDE; CARBON; FRUCTOSE BISPHOSPHATE ALDOLASE; N ACETYLNEURAMINATE LYASE; PYRUVIC ACID; SIALIC ACID; TYROSINE; UNCLASSIFIED DRUG; LIGAND; LYASE; N ACETYLNEURAMINIC ACID; PROTEIN BINDING;

ARTICLE; CHEMICAL BINDING; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; LIGAND BINDING; MOLECULAR MODEL; PRIORITY JOURNAL; PROTON TRANSPORT; QUANTUM MECHANICS; REACTION ANALYSIS; SIMULATION; SITE DIRECTED MUTAGENESIS; STEADY STATE; STEREOSPECIFICITY; THREE DIMENSIONAL IMAGING; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ENZYME ACTIVE SITE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

CATALYTIC DOMAIN; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; LIGANDS; LYASES; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS; N-ACETYLNEURAMINIC ACID; PROTEIN BINDING;

EID: 84899082484     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb500067z     Document Type: Article

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