Structural insights into substrate specificity in variants of N-acetylneuraminic acid lyase produced by directed evolution

Journal of Molecular Biology

Volumn 404, Issue 1, 2010, Pages 56-69

  Campeotto, Ivan ^a   Bolt, Amanda H ^a   Harman, Thomas A ^a   Dennis, Caitriona ^a   Trinh, Chi H ^a   Phillips, Simon E V ^a   Nelson, Adam ^a   Pearson, Arwen R ^a   Berry, Alan ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Directed evolution; N acetylneuraminic acid lyase; Protein engineering; Substrate specificity; X ray crystallography

Indexed keywords

2,3,4 TRIHYDROXY N,N DIPROPYLBUTANAMIDE; BACTERIAL ENZYME; LYASE; LYASE INHIBITOR; N ACETYLNEURAMINATE LYASE; PYRUVIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENETIC VARIABILITY; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS; WILD TYPE;

ESCHERICHIA COLI;

EID: 78049434868     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.08.008     Document Type: Article

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