Statistical analysis of comet assay data

Issues in Toxicology

Volumn , Issue , 2009, Pages 424-450

  Lovell, David P ^a  

^a UNIVERSITY OF SURREY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84898754008     PISSN: 17577179     EISSN: 17577187     Source Type: Book Series    
DOI: None     Document Type: Article

References (63)

1. S. Brendler-Schwaab, A. Hartmann, S. Pfuhler and G. Speit, The in vivo Comet assay: use and status in genotoxicity testing, Mutagenesis, 2005, 20, 245-254.
2. B. Burlinson, R. R. Tice, G. Speit, E. Agurell, S. Y. Brendler-Schwaab, A. R. Collins, P. Escobar, M. Honma, M. T. S. Kumaravel, M. Nakajima, Y. F. Sasaki, V. Thybaud, Y. Uno, M. Vasquez and A. Hartmann, In vivo Comet Assay Workgroup, part of the Fourth International Workgroup on Genotoxicity Testing.) Fourth International Workgroup on Genotoxicity Testing: result of the in vivo Comet assay workgroup, Mutation Research 627 31-35.
3. A. R. Collins, The Comet assay for DNA damage and repair: principles, applications, and limitations, Mol. Biotechnol., 2004, 26, 249-261.
4. A. R. Collins, A. A. Oscoz, G. Brunborg, I. Isabel Gaivão, L. Giovannelli, M. Marcin Kruszewski, C. C. Smith and R. Stetina, The Comet assay: topical issues, Mutagenesis, 2008, 23, 143-151.
5. N. P. Singh, M. T. McCoy, R. R. Tice and E. L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 1988, 75, 184-191.
6. R. R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J. C. Ryu and Y. F. Sasaki, Single cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing, Environ. Mol. Mutagen., 2000, 35, 206-221.
7. A. Hartmann, E. Agurell, C. Beevers, S. Brendler-Schwaab, B. Burlinson, P. Clay, A. Collins, A. Smith, G. Speit, V. Thybaud and R. R. Tice, 4th International Comet Assay Workshop. Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop, Mutagenesis, 2003, 18, 45-51.
8. D. P. Lovell, G. Thomas and R. Dubow, Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies, Teratogenesis Carcinog. Mutagen., 1999, 19, 109-119.
9. D. P. Lovell and T. Omori, Statistical issues in the use of the Comet assay, Mutagenesis, 2008, 23, 171-182.
10. R. A. Fisher and F. Yates, Statistical Tables for Biological, Agricultural and Medical Research, 6th edn, Oliver and Boyd, Edinburgh, 1963.
11. M. J. Gardner and D. Altman, Confidence intervals rather than P values: estimation rather than hypothesis testing, Br. Med. J., 1986, 292, 746-750.
12. D. G. Altman, T. N. Bryant, M. J. Gardner and D. Machin (eds), Statistics with Confidence-Confidence Intervals and Statistical Guidelines, 2nd edn., BMJ Books, London, 2000.
13. D. G. Altman, S. M. Gore, M. J. Gardner and S. J. Pocock, Statistical guidelines for contributors to medical journals, Br. Med. J., 1983, 286, 1489-1493.
14. A. B. Hill, The environment and disease: Association or causation? Proc. R. Soc. Med., 1965, 58, 295-300.
15. J. B. Copas and H. G. Li, Inference for non-random samples (with discussion), J. Roy. Stat. Soc., 1997, 59, 55-95.
16. R. A. Fisher, Statistical Methods for Research Workers. Oliver and Boyd, Edinburgh, 1925.
17. R. A. Fisher, Design of Experiments. Oliver and Boyd, Edinburgh, 1935.
18. G. E. P. Box, W. G. Hunter and J. S. Hunter, Statistics for Experimenters. An Introduction to Design, Data Analysis, and Model Building, 2nd edn., Wiley, 2005.
19. A. Collins, M. Dusinská, M. Franklin, M. Somorovská, H. Petrovská, S. Duthie, L. Fillion, M. Panayiotidis, K. Raslová and N. Vaughan, Comet assay in human biomonitoring studies: reliability, validation, and applications, Environ. Mol. Mutagen., 1997, 30, 139-146.
20. M. Pitarque, A. Creus, R. Marcos, J. A. Hughes and D. Anderson, Examination of various biomarkers measuring genotoxic endpoints from Barcelona airport personnel, Mutation Research, 1999, 440, 195-204.
21. R. D. Bowden, M. R. Buckwalter, J. F. McBride, D. A. Johnson, B. K. Murray and K. L. O'Neill, Tail profile: a more accurate system for analyzing DNA damage using the Comet assay, Mutation Research, 2003, 537, 1-9.
22. F. Forchhammer, E. V. Bräuner, J. K. Folkmann, P. H. Danielsen, C. Nielsen, A. Jensen, S. Loft, G. Friis and P. Møller, Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the Comet assay with visual scoring, Mutagenesis, 2008, 23, 223-231.
23. S. J. Wiklund and E. Agurell, Aspects of design and statistical analysis in the Comet assay, Mutagenesis, 2003, 18, 167-175.
24. T. Çavas and S. Könen, Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the Comet assay, Mutagenesis, 2007, 22, 263-268.
25. S. H. Hurlbert, Pseudoreplication and the design of ecological field experiments, Ecol. Monographs, 1984, 54, 187-211.
26. S. H. Hurlbert, On misinterpretations of pseudoreplication and related matters: a reply to Oksanen, Oikos, 2003, 104, 591-597.
27. L. Machlis, P. W. D. Dodd and J. C. Fentress, The pooling fallacy: problems arising when individuals contribute more than one observation to the dataset, Z. Tierpsychol., 1985, 68, 201-214.
28. C. C. Smith, D. J. Adkins, E. A. Martin and M. R. O'Donovan, Recommendations for design of the rat Comet assay, Mutagenesis, 2008, 23, 233-240.
29. P. Duez, G. Dehon, A. Kumps and J. Dubois, Statistics of the Comet assay: a key to discriminate between genotoxic effects, Mutagenesis, 2003, 18, 159-166.
30. P. A. Escobar, M. T. Smith, A. Vasishta, A. E. Hubbard and L. Zhang, Leukaemia-specific chromosome damage detected by comet with fluorescence in situ hybridization (Comet-FISH), Mutagenesis, 2007, 22, 321-327.
31. T. P. Ryan, Statistical Methods for Quality Improvement, 2nd edn., John Wiley and Sons, New York, 2000.
32. E. Mullins, Statistics for the Quality Control Chemistry Laboratory, Royal Society of Chemistry, Cambridge, 2003.
33. N. T. Longford and J. A. Nelder, Statistics versus statistical science in the regulatory process, Stat. Med., 1999, 18, 2311-2320.
34. M. R. Nester, An Applied Statistician's Creed, Appl. Stat., 1996, 45, 401-410.
35. D. G. Altman and P. Royston, The cost of dichotomising continuous variables, Br. Med. J., 2006, 332, 1080.
36. D. Kirkland, M. Aardema, L. Henderson and L. Müller, Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. I. Sensitivity, specificity and relative predictivity, Mutation Research, 2005, 584, 1-256.
37. J. E. De Muth, Basic Statistics and Pharmaceutical Statistical Applications, 2nd edn., Chapman and Hall/CRC, Boca Raton, FL, 2006.
38. S. W. Lagakos, The Challenge of Subgroup Analyses - Reporting without Distorting, New Eng. J. Med., 2006, 354, 1167-1669.
39. A. Mastaloudis, T.-W. Yu, R. P. O'Donnell, B. Frei, R. Dashwood and M. Traber, Endurance exercise results in DNA damage as detected by the Comet assay, Free Rad. Biol. Med., 2004, 36, 966-975.
40. J. A. Nelder, Contribution to the Discussion of R. T. O'Neill and B. G. Wetherill. The present state of multiple comparison methods, J. Roy. Stat. Soc., B, 1971, 33, 218-241.
41. K. J. Rothman, No adjustments are needed for multiple comparisons, Epidemiology, 1990, 1, 43-46.
42. S. Greenland, Multiple comparisons and association selection in general epidemiology, Int. J. Epidemiol., 2008, 37, 430-434.
43. D. M. Witten and R. Tibshirani (2007) A comparison of fold-change and the t-statistic for microarray data analysis. Posted at http://wwwstat.stanford. edu/Btibs/ftp/FCTComparison.pdf).
44. W.-U. Müller, T. Bauch, C. Streffer and D. Von Mallek, Does radiotherapy affect the outcome of the Comet assay? Br. J. Radiol., 2002, 75, 608-614.
45. J. Cohen, Statistical Power Analysis for the Behavioral Sciences, 2nd edn., Academic Press, New York, 1988.
46. J. Lenth, Statistical power calculations, Anim. Sci., 2007, 85, E24-E29.
47. D. Machin, M. Campbell, P. Fayers and A. Pinol, Sample size tables for clinical studies, 2nd edn., Blackwell Science, Oxford, 1997.
48. S.-C. Chow, S. Jun. and W. Hansheng, Sample-size calculations in Clinical Research, CRC Press, Taylor and Francis Group, Boca Raton, 2003.
49. S. M. Kerry and J. M. Bland, Statistics Notes. Sample size in cluster randomisation, Br. Med. J., 1998, 316, 549.
50. M. K. Campbell, M. S. Thomson, C. R. Ramsay, G. S. MacLennan and J. M. Grimshaw, Sample-size calculator for cluster randomized trials, Comput. Biol. Med., 2004, 34, 113-125.
51. N. T. Holland, M. T. Smith, B. Eskenazi and M. Bastaki, Biological sample collection and processing for molecular epidemiological studies, Mutat. Res., 2003, 543, 217-234.
52. G. R. Wasson, V. J. McKelvey-Martin and C. S. Downes, The use of the Comet assay in the study of human nutrition and cancer, Mutagenesis, 2008, 23, 153-162.
53. M. Mûllner, H. Matthews and D. G. Altman, Reporting on statistical methods to adjust for confounding: a cross-sectional survey, Ann. Internal Med., 2002, 136, 122-126.
54. M. J. Campbell, Statistics at square two: understanding modern statistical applications in medicine, BMJ Publishing Group, London, 2001.
55. M. Dusinska and A. R. Collins, The Comet assay in human biomonitoring: gene-environment interactions, Mutagenesis, 2008, 23, 191-205.
56. A. N. Jha, Ecotoxicological applications and significance of the Comet assay, Mutagenesis, 2008, 23, 207-221.
57. D. J. McKenna, S. R. McKeown and V. J. McKelvey-Martin, Potential use of the Comet assay in the clinical management of cancer, Mutagenesis, 2008, 23, 183-190.
58. S. E. Taube, J. W. Jacobson and T. G. Lively, Cancer Diagnostics: Decision Criteria for Marker Utilization in the Clinic. Molecular Diagnostics, Am. J. Pharmacogenomics, 2005, 5, 357-364.
59. L. M. McShane, D. G. Altman, W. Sauerbrei, S. E. Taube, M. Gion and G. M. Clark, Reporting Recommendations for Tumor Marker Prognostic Studies (REMARK), Breast Cancer Res. Treat., 2006, 100, 229-235.
60. N. J. Wald and J. K. Morris, Teleoanalysis: combining data from different types of study, Br. Med. J., 2003, 327, 616-618.
61. ESCODD (European Standards Committee on Oxidative DNA Damage). C. M. Gedik and A. Collins, Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study, FASEB, 2005, 19, 82-84.
62. International Standards Organization (ISO) Precision of test methods - Determination of repeatability and reproducibility for a standard test by inter-laboratory tests. ISO 5726, 1986.
63. American Society for Testing and Materials, ASTM E691-99 Standard practice for conducting an Interlaboratory Study to determine the precision of a test method. ASTM 10 May 1999.