Effect of glutamine substitution by TCA cycle intermediates on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture

Journal of Biotechnology

Volumn 180, Issue , 2014, Pages 23-29

  Ha, Tae Kwang ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Alpha ketoglutarate; Ammonia; CHO cells; Glutamine; Sialylation

Indexed keywords

AMMONIA; CARBOXYLIC ACIDS; CELL CULTURE; ENZYME ACTIVITY; GLYCOPROTEINS; GLYCOSYLATION; PROTEINS;

ALPHA-KETOGLUTARATE; AMMONIA CONCENTRATIONS; CHINESE HAMSTER OVARY CELLS; CHO CELL; GLUTAMINE; PRODUCT CONCENTRATION; PRODUCTION PERFORMANCE; SIALYLATION;

AMINO ACIDS;

2 OXOGLUTARIC ACID; ALPHA-KETOGLUTARIC ACID; AMMONIA; BETA-GALACTOSIDE ALPHA-2,3-SIALYLTRANSFERASE; CULTURE MEDIUM; GLUTAMINE; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; N ACETYLNEURAMINIC ACID; SIALIDASE; SIALYLTRANSFERASE;

ANIMAL; CELL PROLIFERATION; CELL SURVIVAL; CHEMISTRY; CHO CELL LINE; CITRIC ACID CYCLE; CRICETULUS; CULTURE MEDIUM; DRUG EFFECTS; HAMSTER; METABOLISM; PHARMACOLOGY; PHYSIOLOGY;

AMMONIA; ANIMALS; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CITRIC ACID CYCLE; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLUTAMINE; IMMUNOGLOBULIN FC FRAGMENTS; KETOGLUTARIC ACIDS; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; RECOMBINANT FUSION PROTEINS; SIALYLTRANSFERASES;

EID: 84898685251     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.04.002     Document Type: Article

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