Cloning, expression and functional validation of a β- fructofuranosidase from Lactobacillus plantarum

Process Biochemistry

Volumn 49, Issue 5, 2014, Pages 758-767

  Chen, Chen ^a,b   Zhou, Fangfang ^a   Ren, Jing ^a   Ai, Lianzhong ^a   Dong, Yiying ^a   Wu, Zhengjun ^a   Liu, Zhenmin ^a   Chen, Wei ^b   Guo, Benheng ^a  

^a State Key Laboratory of Dairy Biotechnology   (China)

^b JIANGNAN UNIVERSITY   (China)

Author keywords

Fructooligosaccharides; Glycoside hydrolase family 32; Lactobacillus plantarum; Fructofuranosidase

Indexed keywords

AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; POLYSACCHARIDES; STRAIN; SUGAR (SUCROSE);

FRUCTO-OLIGOSACCHARIDES; FUNCTIONAL VALIDATION; GLYCOSIDE HYDROLASES; LACTOBACILLUS PLANTARUM; LACTOBACILLUS RHAMNOSUS; OPTIMAL REACTION TEMPERATURE; SUBSTRATE HYDROLYSIS; THREE-DIMENSIONAL STRUCTURE;

BACILLI;

ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS PLANTARUM; LACTOBACILLUS RHAMNOSUS;

EID: 84898441038     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2014.02.013     Document Type: Article

References (35)

1. T.R. Callaway, T.S. Edrington, R.C. Anderson, R.B. Harvey, K.J. Genovese, and C.N. Kennedy et al. Probiotics, prebiotics and competitive exclusion for prophylaxis against bacterial disease Anim Health Res Rev 9 2008 217 225
2. G.R. Gibson, and M.B. Roberfroid Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics J Nutr 125 1995 1401 1412
3. R. Barrangou, E. Altermann, R. Hutkins, R. Cano, and T.R. Klaenhammer Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus Proc Natl Acad Sci U S A 100 2003 8957 8962
4. D.M. Saulnier, D. Molenaar, W.M. de Vos, G.R. Gibson, and S. Kolida Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays Appl Environ Microbiol 73 2007 1753 1765
5. K.E. Bach Knudsen, and I. Hessov Recovery of inulin from Jerusalem artichoke (Helianthus tuberosus L.) in the small intestine of man Br J Nutr 74 1995 101 113
6. Y. Bouhnik, K. Vahedi, L. Achour, A. Attar, J. Salfati, and P. Pochart et al. Short-chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans J Nutr 129 1999 113 116
7. G. Le Blay, C. Michel, H.M. Blottiere, and C. Cherbut Prolonged intake of fructo-oligosaccharides induces a short-term elevation of lactic acid-producing bacteria and a persistent increase in cecal butyrate in rats J Nutr 129 1999 2231 2235
8. M. Jedrzejczak-Krzepkowska, K.L. Tkaczuk, and S. Bielecki Biosynthesis, purification and characterization of β-fructofuranosidase from Bifidobacterium longum KN29.1 Process Biochem 46 2011 1963 1972
9. H. Kaplan, and R.W. Hutkins Fermentation of fructooligosaccharides by lactic acid bacteria and bifidobacteria Appl Environ Microbiol 66 2000 2682 2684
10. Y.J. Goh, C. Zhang, A.K. Benson, V. Schlegel, J.H. Lee, and R.W. Hutkins Identification of a putative operon involved in fructooligosaccharide utilization by Lactobacillus paracasei Appl Environ Microbiol 72 2006 7518 7530
11. S.M. Ryan, G.F. Fitzgerald, and D. van Sinderen Transcriptional regulation and characterization of a novel beta-fructofuranosidase-encoding gene from Bifidobacterium breve UCC2003 Appl Environ Microbiol 71 2005 3475 3482
12. T. Omori, K. Ueno, K. Muramatsu, M. Kikuchi, S. Onodera, and N. Shiomi Characterization of recombinant beta-fructofuranosidase from Bifidobacterium adolescentis G1 Chem Cent J 4 2010 9
13. M. Warchol, S. Perrin, J.P. Grill, and F. Schneider Characterization of a purified beta-fructofuranosidase from Bifidobacterium infantis ATCC 15697 Lett Appl Microbiol 35 2002 462 467
14. M.A. Ehrmann, M. Korakli, and R.F. Vogel Identification of the gene for beta-fructofuranosidase of Bifidobacterium lactis DSM10140(T) and characterization of the enzyme expressed in Escherichia coli Curr Microbiol 46 2003 391 397
15. C. Paludan-Muller, L. Gram, and F.P. Rattray Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish Syst Appl Microbiol 25 2002 13 20
16. C.M. Martel, A.G. Warrilow, C.J. Jackson, J.G. Mullins, R.C. Togawa, and J.E. Parker et al. Expression, purification and use of the soluble domain of Lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans Bioresour Technol 101 2010 4395 4402
17. M. Arenahalli Ningegowda, and P. Siddalingaiya Gurudutt In vitro fermentation of prebiotics by Lactobacillus plantarum CFR 2194: selectivity, viability and effect of metabolites on beta-glucuronidase activity World J Microbiol Biotechnol 28 2012 901 908
18. A. Fiser, and A. Sali Modeller: generation and refinement of homology-based protein structure models Methods Enzymol 374 2003 461 491
19. R.A. Laskowski, J.A. Rullmannn, M.W. MacArthur, R. Kaptein, and J.M. Thornton AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR J Biomol NMR 8 1996 477 486
20. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
21. E. Sorvig, G. Mathiesen, K. Naterstad, V.G. Eijsink, and L. Axelsson High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors Microbiology 151 2005 2439 2449
22. T. Aukrust, and H. Blom Transformation of Lactobacillus strains used in meat and vegetable fermentations Food Res Int 25 1992 253 261
23. D.G. Naumov, and V.A. Livshits Molecular structure of the locus for sucrose utilization by Lactobacillus plantarum: comparison with Pediococcus pentosaceus Mol Biol 35 2001 19 27
24. W.W. Navarre, and O. Schneewind Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope Microbiol Mol Biol Rev 63 1999 174 229
25. M. Alvaro-Benito, M.A. Sainz-Polo, D. Gonzalez-Perez, B. Gonzalez, F.J. Plou, and M. Fernandez-Lobato et al. Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis beta-fructofuranosidase, an enzyme that produces prebiotics J Biol Chem 287 2012 19674 19686
26. A. Bujacz, M. Jedrzejczak-Krzepkowska, S. Bielecki, I. Redzynia, and G. Bujacz Crystal structures of the apo form of beta-fructofuranosidase from Bifidobacterium longum and its complex with fructose FEBS J 278 2011 1728 1744
27. V.A. Reddy, and F. Maley Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis J Biol Chem 265 1990 10817 10820
28. A. Reddy, and F. Maley Studies on identifying the catalytic role of Glu-204 in the active site of yeast invertase J Biol Chem 271 1996 13953 13957
29. F.R. Batista, L. Hernandez, J.R. Fernandez, J. Arrieta, C. Menendez, and R. Gomez et al. Substitution of Asp-309 by Asn in the Arg-Asp-Pro (RDP) motif of Acetobacter diazotrophicus levansucrase affects sucrose hydrolysis, but not enzyme specificity Biochem J 337 1999 503 506
30. F. Alberto, C. Bignon, G. Sulzenbacher, B. Henrissat, and M. Czjzek The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases J Biol Chem 279 2004 18903 18910
31. C. Janer, L.M. Rohr, C. Pelaez, M. Laloi, V. Cleusix, and T. Requena et al. Hydrolysis of oligofructoses by the recombinant beta-fructofuranosidase from Bifidobacterium lactis Syst Appl Microbiol 27 2004 279 285
32. R. Van der Meulen, L. Makras, K. Verbrugghe, T. Adriany, and L. De Vuyst In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp. indicates different degradation mechanisms Appl Environ Microbiol 72 2006 1006 1012
33. G. Falony, A. Vlachou, K. Verbrugghe, and L. De Vuyst Cross-feeding between Bifidobacterium longum BB536 and acetate-converting, butyrate-producing colon bacteria during growth on oligofructose Appl Environ Microbiol 72 2006 7835 7841
34. M. Alvaro-Benito, A. Polo, B. Gonzalez, M. Fernandez-Lobato, and J. Sanz-Aparicio Structural and kinetic analysis of Schwanniomyces occidentalis invertase reveals a new oligomerization pattern and the role of its supplementary domain in substrate binding J Biol Chem 285 2010 13930 13941
35. M.A. Sainz-Polo, M. Ramirez-Escudero, A. Lafraya, B. Gonzalez, J. Marin-Navarro, and J. Polaina et al. Three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity J Biol Chem 288 2013 9755 9766