Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics

Journal of Biological Chemistry

Volumn 287, Issue 23, 2012, Pages 19674-19686

  Álvaro Benito, Miguel ^a   Sainz Polo, M Angela ^b   González Pérez, David ^a   González, Beatriz ^b   Plou, Francisco J ^c   Fernández Lobato, María ^a   Sanz Aparicio, Julia ^b  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

^c INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; DIMERIC ENZYME; EXPERIMENTAL EVIDENCE; FRUCTANS; FRUCTO-OLIGOSACCHARIDES; GROWTH OF BACTERIA; HIGH ACTIVITY; HUMAN HEALTH; MOLECULAR BASIS; PREBIOTICS; RATIONAL DESIGN; SCHWANNIOMYCES OCCIDENTALIS; SUBSITES; SUBSTRATE BINDING; SUGAR UNITS; TRANSFRUCTOSYLATION REACTIONS;

AMINO ACIDS; DIMERS; POLYSACCHARIDES; SUBSTRATES; SUGARS;

ENZYMES;

AMINO ACID; ASPARAGINE; BETA FRUCTOFURANOSIDASE; FRUCTOSE; GLUTAMINE; GLYCINE; INULIN; PREBIOTIC AGENT; SUCROSE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SCHWANNIOMYCES OCCIDENTALIS;

AMINO ACID SUBSTITUTION; BETA-FRUCTOFURANOSIDASE; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; FRUCTOSE; FUNGAL PROTEINS; INULIN; MUTATION, MISSENSE; PREBIOTICS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCETALES; SUBSTRATE SPECIFICITY;

DEBARYOMYCES OCCIDENTALIS;

EID: 84861741136     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.355503     Document Type: Article

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