Antiparallel conformation of knob and hole aglycosylated half-antibody homodimers is mediated by a CH2-CH3 hydrophobic interaction

Journal of Molecular Biology

Volumn 426, Issue 9, 2014, Pages 1947-1957

  Elliott, J Michael ^a   Ultsch, Mark ^a   Lee, Joshua ^a   Tong, Raymond ^a   Takeda, Kentaro ^a   Spiess, Christoph ^a   Eigenbrot, Charles ^a   Scheer, Justin M ^a  

^a GENENTECH INC   (United States)

Author keywords

antibody structure; bispecific antibody; CH3 domain; heterodimer interface; hydrophobic interface

Indexed keywords

BISPECIFIC ANTIBODY; HETERODIMER; HOLE HALF ANTIBODY; HOMODIMER; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1; KNOB HALF ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY STRUCTURE; ARTICLE; BINDING KINETICS; CONFORMATION; CRYSTAL STRUCTURE; DIMERIZATION; ESCHERICHIA COLI; GENE MUTATION; HYDROPHOBIC INTERACTION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS);

ANTIBODY STRUCTURE; BISPECIFIC ANTIBODY; CH3 DOMAIN; HETERODIMER INTERFACE; HYDROPHOBIC INTERFACE;

ANTIBODIES, BISPECIFIC; CRYSTALLOGRAPHY, X-RAY; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; KINETICS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION;

EID: 84898003024     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2014.02.015     Document Type: Article

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