C-terminal protein characterization by mass spectrometry: Isolation of c-terminal fragments from cyanogen bromide-cleaved protein

Journal of Biomolecular Techniques

Volumn 25, Issue 1, 2014, Pages 1-18

  Nika, Heinz ^a   Hawke, David H ^b   Angeletti, Ruth Hogue ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Aminolysis; C terminomics; Covalent chromatography; Peptide; Serial solid phase derivatization; ZipTipC18 pipette tips

Indexed keywords

C TERMINAL PEPTIDE; CYANOGEN BROMIDE; CYSTAMINE; PEPTIDE; UNCLASSIFIED DRUG; PEPTIDE FRAGMENT; PROTEIN;

ACETYLATION; AMINATION; AMINO ACID SEQUENCE; ARTICLE; BENZOYLATION; CHEMICAL MODIFICATION; CHROMATOGRAPHY; CONJUGATION; CONTROLLED STUDY; DERIVATIZATION; DISULFIDE BOND; ISOTOPE LABELING; LACTONIZATION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN IMMOBILIZATION; PROTEIN ISOLATION; PROTEIN METHYLATION; PROTEIN PURIFICATION; CHEMISTRY; MOLECULAR WEIGHT; PROTEIN DEGRADATION;

CYANOGEN BROMIDE; MASS SPECTROMETRY; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PROTEINS; PROTEOLYSIS;

EID: 84897418883     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.14-2501-001     Document Type: Article

References (30)

1. Chung JJ, Shikano S, Hanyu Y, Li M. Functional diversity of protein C-termini: more than zipcoding. Trends Cell Biol 2002; 12:146-150
2. Wilkins MR, Gasteiner E, Tonella L, et al. Protein identification with N and C-terminal sequence tags in proteome projects. J Mol Biol 1998;278:549-608
3. Nakazowa T, Yamaguchi M, Okamura T, Ando E, Nishimura O, Tsunasawa S. Terminal proteomics: N- and C-terminal analyses for high-fidelity identification of proteins using MS. Proteomics 2008;8:673-685
4. Samyn B, Sergeant K, Castanheira P, Faro C, van Beeumen J. A new method for C-terminal sequence analysis in the proteomic era. Nat Methods 2005;2:193-200
5. Julka S, Dielman D, Young SA. Detection of C-terminal peptides of proteins using isotope coded strategies. J Chromatogr 2008;874:101-110
6. Murphy CM, Fenselaue C. Recognition of the caroboxy-treminal peptides in cyanogen bromide digests of proteins. Anal Chem 1995;67:1644-1645
7. Sechi S, Chait BT. A method to define the carboxyl terminus of proteins. Anal Chem 2000;72:2374-3378
8. Kuyama H, Shima K, Sonomura K, et al. A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry. Proteomics 2008;8:1539-1550
9. Sonomura K, Kuyama H, Matsuo E, Tsunasawa S, Nishimura O.The specific isolation of C-terminal peptides of proteins through a transamination reaction and its advantage for introducing functional groups into the peptide. Rapid Commun Mass Spectrom 2009;23:611-618
10. Nika H, Nieves E, Hawke DH, Angeletti RH. C-terminal protein characterization by mass spectrometry using micro scale liquid and solid-phase derivatization. J Biomol Tech 2013;24: 17-31
11. Nika H, Nieves E, Hawke DH, Angeletti RH. Optimization of the β-elimination/Michael addition chemistry on reversedphase supports for mass spectrometry analysis of O-linked protein modifications. J Biomol Tech 2013;24:132-153
12. Rodriguez JC, Jennings PA. The solvent in CNBr cleavage reactions determines the fragmentation efficiency of ketosteroid isomerase fusion proteins used in the production of recombinant peptides. Protein Expr Purif 2003;28:224-231
13. Horn JM, Laursen RA. Solid-phase Edman degradation: attachment of carboxyl-terminal homoserine peptides to an insoluble resin. FEBS Lett 1973;36:285-288
14. Shi T, Weerasekera R, Yan C, et al. Method for affinity purification of covalently linked peptides following cyanogen bromide cleavage of proteins. Anal Chem 2009;81:9885-9895
15. Nika H, Lee JH, Willis IM, Angeletti RH, Hawke DH. Phosphopeptide characterization by mass spectrometry using reversed- phase supports for solid-phase _-elimination/Michael addition. J Biomol Tech 2012;23:2012-2302
16. Speicher DT, Kolbas O, Harper D, Speicher DW. Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies. J Biomol Tech 2000;11:74-86
17. Nika H, Hawke DH, Kobayashi, R. Derivatization on reversedphase supports for enhanced detection of phosphorylated peptides. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, TN, USA, 2004, Abstr ThPN 292
18. Conrotto P, Hellman U. Sulfonation chemistry as a powerful tool for MALDI TOF/TOF de novo sequencing and posttranslational modification analysis. J Biomol Tech 2005;16:441-452
19. Cindric M, Cepo T, Skrlin A, Vuletic M, Bindila L. Accelerated on-column lysine derivatization and cysteine methylation by imidazole reaction in a deuterated environment for enhanced product ion analysis. Rapid Commun Mass Spectrom 2006;20: 694-702
20. Rosefeld JM. Solid-phase analytical derivatization: enhancement of sensitivity and selectivity of analysis. J Chromatogr A 1999;843:19-27
21. Johnson ME, Carpenter TS. The use of solid-phase supports for derivatization in chromatography and spectroscopy. Appl Spectrosc Rev 2005;40:391-412
22. Katayama H, Nagasu T, Oda Y. Improvement of in-gel digestion protocol for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2001;15:1416-1421
23. Cohen SL, Chait BT. Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins. Anal Chem 1996;68:31-37
24. Katayama H, Tabata T, Ishihama Y, Sato T, Oda Y, Nagasu T. Efficient in-gel digestion procedure using 5-cyclohexyl-1-pentyl-β-D-maltoside as an additive for gel-based membrane proteomics. Rapid CommunMass Spectrom 2004;18:2388-2394
25. Brocklehurst K, Carlsson J, Marek P, Kierstan J, Crook EM. Covalent chromatography. Preparation of fully active papain from dried papaya latex. Biochem J 1973;133:573-584
26. Kelleher NL, Lin HY, Valaskovic GA, et al. Top down versus bottom up protein characterization by tandem high-resolution mass spectrometry. J Am Chem Soc 1999;121:806-912
27. Kirkland JJ, van Straten MA, Claessens HA. High pH mobile phase effects on silica-based reversed-phase high-performance liquid chromatography. J Chromatogr A 1995;691:3-19
28. Palmblad M, Vogel JS. Quantitation of binding, recovery and desalting effiency of peptides and proteins in solid phase extraction micropipette tips. J Chromatogr B 2005;814:309-313
29. Takayama N, Matsui T, Sakai T, Tsugita A. A method for peptide successive C-terminal degradation using dilute hydrochloric acid. J Biolmol Tech 1999;10:194-198
30. Panchaud A, Hansson J, Affolter M, et al. ANIBAL, stable isotopebased quantitative proteomics by aniline and benzoic acid labeling of amino and carboxylic groups. Mol Cell Proteomics 2008;7:800-812.