C-terminal protein characterization by mass spectrometry using combined micro scale liquid and solid-phase derivatization

Journal of Biomolecular Techniques

Volumn 24, Issue 1, 2013, Pages 17-31

  Nika, H ^a   Nieves, E ^a   Hawke, David H ^b   Angeletti, Ruth Hogue ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

C terminal peptide identification; C terminal peptide structural characterization; Multistep derivatization; Reversed phase supports

Indexed keywords

BETA LACTOGLOBULIN; FIBRINOPEPTIDE B; SEPHAROSE; TRYPSIN;

ACETYLATION; AFFINITY CHROMATOGRAPHY; AMINATION; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; DERIVATIZATION; ELUTION; GEL FILTRATION; IMMOBILIZATION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYMERIZATION; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN METHYLATION; SEQUENCE ANALYSIS;

C-TERMINAL PEPTIDE IDENTIFICATION; C-TERMINAL PEPTIDE STRUCTURAL CHARACTERIZATION; MULTISTEP DERIVATIZATION; REVERSED-PHASE SUPPORTS;

ACETYLATION; AMINO ACID SEQUENCE; BRADYKININ; FIBRINOPEPTIDE B; HUMANS; MASS SPECTROMETRY; PEPTIDE FRAGMENTS; PEPTIDES; PROTEINS; SOLID-PHASE SYNTHESIS TECHNIQUES; TRYPSIN;

EID: 84875727387     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.13-2401-003     Document Type: Article

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