Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry

Nature Protocols

Volumn 9, Issue 4, 2014, Pages 828-841

  Su, Dan ^a,d   Chan, Clement T Y ^a,e   Gu, Chen ^a   Lim, Kok Seong ^a,f   Chionh, Yok Hian ^b   McBee, Megan E ^b   Russell, Brandon S ^a   Babu, I Ramesh ^a   Begley, Thomas J ^c   Dedon, Peter C ^a,b  

^a Massachusetts Institute of Technology   (United States)

^b SINGAPORE MIT ALLIANCE FOR RESEARCH AND TECHNOLOGY   (Singapore)

^c STATE UNIVERSITY OF NEW YORK   (United States)

^d Thermo Fisher Scientific   (United States)

^e Boston University   (United States)

^f RA Capital Management   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; RIBONUCLEOSIDE; TRANSFER RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL STRESS; CHEMICAL MODIFICATION; CHEMICAL PARAMETERS; CLINICAL PROTOCOL; DYNAMIC MULTIPLE REACTION MONITORING; GENETIC ENGINEERING AND GENE TECHNOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HYDROLYSIS; INTERMETHOD COMPARISON; MASS SPECTROMETRY; MEASUREMENT ACCURACY; MONITORING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PURIFICATION; QUANTITATIVE ANALYSIS; RETENTION TIME; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; RIBONUCLEOSIDE MODIFICATION; RNA ISOLATION; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION; CONTROLLED STUDY; HUMAN CELL; PROCESS DEVELOPMENT; PROCESS MONITORING; RNA ANALYSIS; RNA MODIFICATION; RNA PROCESSING; RNA PURIFICATION; RNA STRUCTURE; VALIDATION PROCESS; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MASS SPECTROMETRY; RIBONUCLEOSIDES; RNA, TRANSFER;

EID: 84897129376     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.047     Document Type: Article

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