Single-cell PCR analysis of murine embryonic stem cells cultured on different substrates highlights heterogeneous expression of stem cell markers

Biology of the Cell

Volumn 105, Issue 12, 2013, Pages 549-560

  Franzin, Chiara ^a   Piccoli, Martina ^a   Serena, Elena ^b   Bertin, Enrica ^a   Urbani, Luca ^a   Luni, Camilla ^b,c   Pasqualetto, Valerie ^d,e   Eaton, Simon ^f   Elvassore, Nicola ^b,c   De Coppi, Paolo ^b,f   Pozzobon, Michela ^a  

^a Fondazione Istituto di Ricerca Pediatrica Città della Speranza   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

^c VENETIAN INSTITUTE OF MOLECULAR MEDICINE   (Italy)

^d UNIVERSITÉ PARIS DESCARTES   (France)

^e INSERM   (France)

^f UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Feeder layer free cultures; Single cell PCR; Stem cells markers; Stiffness

Indexed keywords

CELL MARKER; KRUPPEL LIKE FACTOR 4; MATRIGEL; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; RNA 28S; STEM CELL ANTIGEN 1; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL FATE; CELL FUNCTION; CELL LEVEL; CELL POPULATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; FEEDER CELL; FIBROBLAST; GENE EXPRESSION; MALE; MOUSE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SINGLE CELL ANALYSIS; STEM CELL CULTURE;

MURINAE;

FEEDER LAYER-FREE CULTURES; SINGLE-CELL PCR; STEM CELLS MARKERS; STIFFNESS;

ANIMALS; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; CULTURE MEDIA; DNA PRIMERS; EMBRYONIC STEM CELLS; FEEDER CELLS; FIBROBLASTS; MALE; MICE; POLYMERASE CHAIN REACTION; SINGLE-CELL ANALYSIS;

EID: 84897068181     PISSN: 02484900     EISSN: 1768322X     Source Type: Journal    
DOI: 10.1111/boc.201300034     Document Type: Article

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