SCOPUS 정보 검색 플랫폼

Volumn 397, Issue 5, 2010, Pages 1853-1859

An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells

(8) Li, Yongzhong a Thompson, Hansa a Hemphill, Courtney a Hong, Fan a Forrester, Jessica a Johnson, Roger H a Zhang, Weiwen a Meldrum, Deirdre R a

a ARIZONA STATE UNIVERSITY (United States)

Author keywords

Gene expression; One tube; RT PCR; Single cell

Indexed keywords

CANCER CELLS; CELL LINES; CELL LYSIS; CELL POPULATIONS; CELL REGULATION; DIRECT MEASUREMENT; EXPRESSION LEVELS; GENETIC DIAGNOSTICS; HIGH-THROUGHPUT; INDIVIDUAL CELLS; INDIVIDUAL DIFFERENCES; INTERCALATORS; MAMMALIAN CELLS; NESTED PCR; ONE-TUBE; ORDERS OF MAGNITUDE; Q-RT-PCR; REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTIONS; RNA ISOLATION; RNA STABILITY; SINGLE-CELL LEVEL; SYBR GREEN; TRANSCRIPTION LEVEL;

CELL CULTURE; CELL PROLIFERATION; MAMMALS; POLYMERASE CHAIN REACTION; RNA; TRANSCRIPTION; TUBES (COMPONENTS);

GENE EXPRESSION REGULATION;

ARTICLE; CELL; CHEMISTRY; CYTOLOGY; EVALUATION; GENE EXPRESSION; HUMAN; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE; CELLS; EVALUATION STUDY; PROCEDURES;

CELL LINE, TUMOR; CELLS; GENE EXPRESSION; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

MAMMALIA;

EID: 77953811012 PISSN: 16182642 EISSN: 16182650 Source Type: Journal
DOI: 10.1007/s00216-010-3754-0 Document Type: Article

Times cited : (17)

References (37)

1
- 0030906790
- 1:CAS:528:DyaK2sXjtFCmu7Y%3D
- TG Eddinger DP Meer 1997 Comp Biochem Physiol 117B 29 38 1:CAS:528:DyaK2sXjtFCmu7Y%3D
- (1997) Comp Biochem Physiol , vol.117 , pp. 29-38
- Eddinger, T.G.¹ Meer, D.P.²

2
- 1642460171
- 10.1038/nrmicro755 1:CAS:528:DC%2BD3sXosl2hsrY%3D
- ME Lidstrom DR Meldrum 2003 Nat Rev Microbiol 1 158 164 10.1038/nrmicro755 1:CAS:528:DC%2BD3sXosl2hsrY%3D
- (2003) Nat Rev Microbiol , vol.1 , pp. 158-164
- Lidstrom, M.E.¹ Meldrum, D.R.²

3
- 33747047561
- Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regular of stem cell quiescence
- DOI 10.1073/pnas.0601886103
- KB Jensen FM Watt 2006 Proc Natl Acad Sci USA 103 11958 11963 10.1073/pnas.0601886103 1:CAS:528:DC%2BD28XosVSgsbg%3D (Pubitemid 44215793)
- (2006) Proceedings of the National Academy of Sciences of the United States of America , vol.103 , Issue.32 , pp. 11958-11963
- Jensen, K.B.¹ Watt, F.M.²

4
- 0028328719
- 10.1038/icb.1994.26 1:STN:280:DyaK2c3lvFCktA%3D%3D
- IL Ross CM Browne DA Hume 1994 Immunol Cell Biol 72 177 185 10.1038/icb.1994.26 1:STN:280:DyaK2c3lvFCktA%3D%3D
- (1994) Immunol Cell Biol , vol.72 , pp. 177-185
- Ross, I.L.¹ Browne, C.M.² Hume, D.A.³

5
- 0037119587
- Stochastic gene expression in a single cell
- DOI 10.1126/science.1070919
- MB Elowitz AJ Levine ED Siggia PS Swain 2002 Science 297 1183 1186 10.1126/science.1070919 1:CAS:528:DC%2BD38XmsVOht7c%3D (Pubitemid 36193353)
- (2002) Science , vol.297 , Issue.5584 , pp. 1183-1186
- Elowitz, M.B.¹ Levine, A.J.² Siggia, E.D.³ Swain, P.S.⁴

6
- 0242416539
- Noise in eukaryotic gene expression
- DOI 10.1038/nature01546
- WJ Blake M Kaern CR Cantor JJ Collins 2003 Nature 422 633 637 10.1038/nature01546 1:CAS:528:DC%2BD3sXislCgtLc%3D (Pubitemid 36460419)
- (2003) Nature , vol.422 , Issue.6932 , pp. 633-637
- Blake, W.J.¹ Kaern, M.² Cantor, C.R.³ Collins, J.J.⁴

7
- 33645027986
- 10.1038/nature04599 1:CAS:528:DC%2BD28XitlKgu7k%3D
- L Cai N Friedman XS Xie 2006 Nature 440 358 362 10.1038/nature04599 1:CAS:528:DC%2BD28XitlKgu7k%3D
- (2006) Nature , vol.440 , pp. 358-362
- Cai, L.¹ Friedman, N.² Xie, X.S.³

8
- 34347260600
- Living with noisy genes: How cells function reliably with inherent variability in gene expression
- DOI 10.1146/annurev.biophys.36.040306.132705
- N Maheshri EK O'Shea 2007 Ann Rev Biophys Biomol Struct 36 413 434 10.1146/annurev.biophys.36.040306.132705 1:CAS:528:DC%2BD2sXnsVahu7Y%3D (Pubitemid 46998126)
- (2007) Annual Review of Biophysics and Biomolecular Structure , vol.36 , pp. 413-434
- Maheshri, N.¹ O'Shea, E.K.²

9
- 33748994704
- Stochastic mRNA synthesis in mammalian cells
- DOI 10.1371/journal.pbio.0040309
- A Raj CS Peskin D Tranchina DY Vargas S Tyagi 2006 PLoS Biol 4 e309 10.1371/journal.pbio.0040309 (Pubitemid 44610091)
- (2006) PLoS Biology , vol.4 , Issue.10 , pp. 1707-1719
- Raj, A.¹ Peskin, C.S.² Tranchina, D.³ Vargas, D.Y.⁴ Tyagi, S.⁵

10
- 34248662082
- Split single-cell RT-PCR analysis of Purkinje cells
- DOI 10.1038/nprot.2006.343, PII NPROT.2006.343
- S Esumi R Kaneko Y Kawamura T Yagi 2006 Nat Protoc 1 2143 2151 10.1038/nprot.2006.343 1:CAS:528:DC%2BD2sXhtFagtrrO (Pubitemid 46773337)
- (2006) Nature Protocols , vol.1 , Issue.4 , pp. 2143-2151
- Esumi, S.¹ Kaneko, R.² Kawamura, Y.³ Yagi, T.⁴

11
- 33644914709
- 10.1016/j.mam.2005.12.007 1:CAS:528:DC%2BD28XisF2gsL4%3D
- M Kubista JM Andrade M Bengtsson A Forootan J Jonak K Lind R Sindelka R Sjöback B Sjögreen L Strömbom A Ståhlberg N Zoric 2006 Mol Aspects Med 27 95 125 10.1016/j.mam.2005.12.007 1:CAS:528:DC%2BD28XisF2gsL4%3D
- (2006) Mol Aspects Med , vol.27 , pp. 95-125
- Kubista, M.¹ Andrade, J.M.² Bengtsson, M.³ Forootan, A.⁴ Jonak, J.⁵ Lind, K.⁶ Sindelka, R.⁷ Sjöback, R.⁸ Sjögreen, B.⁹ Strömbom, L.¹⁰ Ståhlberg, A.¹¹ Zoric, N.¹²

12
- 33847643776
- 10.1038/nprot.2006.236 1:CAS:528:DC%2BD28Xht1eitLzM
- T Nolan RE Hands SA Bustin 2006 Nat Protoc 1 1559 1582 10.1038/nprot.2006.236 1:CAS:528:DC%2BD28Xht1eitLzM
- (2006) Nat Protoc , vol.1 , pp. 1559-1582
- Nolan, T.¹ Hands, R.E.² Bustin, S.A.³

13
- 39549088479
- Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos
- DOI 10.1186/1472-6750-7-87
- C Hartshorn J Eckert O Hartung L Wangh 2007 BMC Biotechnol 7 87 10.1186/1472-6750-7-87 (Pubitemid 351280040)
- (2007) BMC Biotechnology , vol.7 , pp. 87
- Hartshorn, C.¹ Eckert, J.J.² Hartung, O.³ Wangh, L.J.⁴

14
- 0036808209
- Single cell RT-PCR analysis of tyrosine kinase receptor expression in adult rat retinal ganglion cells isolated by retinal sandwiching
- DOI 10.1016/S1385-299X(02)00184-8, PII S1385299X02001848
- N Lindqvist M Vidal-Sanz F Hallbook 2002 Brain Res Protoc 10 75 83 10.1016/S1385-299X(02)00184-8 1:CAS:528:DC%2BD38XosFCku7w%3D (Pubitemid 35335973)
- (2002) Brain Research Protocols , vol.10 , Issue.2 , pp. 75-83
- Lindqvist, N.¹ Vidal-Sanz, M.² Hallbook, F.³

15
- 50649117806
- 10.1152/japplphysiol.00897.2007 1:CAS:528:DC%2BD1cXps12htbg%3D
- MJ Wacker MM Tehel PM Gallagher 2008 J Appl Physiol 105 308 315 10.1152/japplphysiol.00897.2007 1:CAS:528:DC%2BD1cXps12htbg%3D
- (2008) J Appl Physiol , vol.105 , pp. 308-315
- Wacker, M.J.¹ Tehel, M.M.² Gallagher, P.M.³

16
- 0026588147
- 10.1073/pnas.89.7.3010 1:CAS:528:DyaK38XitVyrs7g%3D
- J Eberwine H Yeh K Miyashiro Y Cao S Nair R Finnell M Zettel P Coleman 1992 Proc Natl Acad Sci USA 89 3010 3014 10.1073/pnas.89.7.3010 1:CAS:528:DyaK38XitVyrs7g%3D
- (1992) Proc Natl Acad Sci USA , vol.89 , pp. 3010-3014
- Eberwine, J.¹ Yeh, H.² Miyashiro, K.³ Cao, Y.⁴ Nair, S.⁵ Finnell, R.⁶ Zettel, M.⁷ Coleman, P.⁸

17
- 0034626872
- 10.1097/00001756-200011090-00035 1:CAS:528:DC%2BD3cXosFKnur0%3D
- KV Christensen DWM Lambert EJ JDC 2000 NeuroReport 11 3577 3582 10.1097/00001756-200011090-00035 1:CAS:528:DC%2BD3cXosFKnur0%3D
- (2000) NeuroReport , vol.11 , pp. 3577-3582
- Christensen, K.V.¹ Lambert, D.W.M.² Jdc, E.J.³

18
- 40649084023
- 10.1016/j.neures.2007.12.011 1:CAS:528:DC%2BD1cXjtlWit7g%3D
- S Esumi SX Wu Y Yanagawa K Obata Y Sugimoto N Tamamaki 2008 Neurosci Res 60 439 451 10.1016/j.neures.2007.12.011 1:CAS:528:DC%2BD1cXjtlWit7g%3D
- (2008) Neurosci Res , vol.60 , pp. 439-451
- Esumi, S.¹ Wu, S.X.² Yanagawa, Y.³ Obata, K.⁴ Sugimoto, Y.⁵ Tamamaki, N.⁶

19
- 0035006682
- 1:CAS:528:DC%2BD3MXjtFOitr8%3D
- N Faumont TA Saati P Brousset C Offer G Delson F Meggetto 2001 J Gen Virol 82 1169 1174 1:CAS:528:DC%2BD3MXjtFOitr8%3D
- (2001) J Gen Virol , vol.82 , pp. 1169-1174
- Faumont, N.¹ Saati, T.A.² Brousset, P.³ Offer, C.⁴ Delson, G.⁵ Meggetto, F.⁶

20
- 0031656064
- 10.1038/sj.leu.2401154
- T Miragi K Murakami T Sawada H Taguchi I Miyoshi 1998 Leukemia 12 1645 1650 10.1038/sj.leu.2401154
- (1998) Leukemia , vol.12 , pp. 1645-1650
- Miragi, T.¹ Murakami, K.² Sawada, T.³ Taguchi, H.⁴ Miyoshi, I.⁵

21
- 0036724945
- 10.1093/nar/gnf088
- B Liss 2002 Nucleic Acids Res 30 e89 10.1093/nar/gnf088
- (2002) Nucleic Acids Res , vol.30 , pp. 89
- Liss, B.¹

22
- 0037093088
- One-tube protocol for single-cell reverse transcriptase-polymerase chain reaction
- DOI 10.1006/abio.2002.5623
- L Yan G Kaczorowski M Kohler 2002 Anal Biochem 304 267 270 10.1006/abio.2002.5623 1:CAS:528:DC%2BD38XjsFOgs70%3D (Pubitemid 34522973)
- (2002) Analytical Biochemistry , vol.304 , Issue.2 , pp. 267-270
- Yan, L.¹ Kaczorowski, G.² Kohler, M.³

23
- 2342622835
- Real-Time PCR for Single-Cell Genotyping in Sickle Cell and Thalassemia Syndromes as a Rapid, Accurate, Reliable, and Widely Applicable Protocol for Preimplantation Genetic Diagnosis
- DOI 10.1002/humu.20022
- C Vrettou J Traeger-Synodinos M Tzetis G Palmer C Sofocleous E Kanavakis 2004 Hum Mutat 23 513 521 10.1002/humu.20022 1:CAS:528:DC%2BD2cXkslyhtrs%3D (Pubitemid 38569225)
- (2004) Human Mutation , vol.23 , Issue.5 , pp. 513-521
- Vrettou, C.¹ Traeger-Synodinos, J.² Tzetis, M.³ Palmer, G.⁴ Sofocleous, C.⁵ Kanavakis, E.⁶

24
- 6344291988
- Quantification of multiple gene expression in individual cells
- DOI 10.1101/gr.2890204
- A Peixoto M Monteiro B Rocha H Veiga-Fernandes 2004 Genome Res 14 1938 1947 10.1101/gr.2890204 1:CAS:528:DC%2BD2cXotl2hu7k%3D (Pubitemid 39386508)
- (2004) Genome Research , vol.14 , Issue.10 A , pp. 1938-1947
- Peixoto, A.¹ Monteiro, M.² Rocha, B.³ Veiga-Fernandes, H.⁴

25
- 67649586670
- 10.1038/nmeth.1338 1:CAS:528:DC%2BD1MXnt1Sht7Y%3D
- K Taniguchi T Kajiyama H Kambara 2009 Nat Meth 6 503 506 10.1038/nmeth.1338 1:CAS:528:DC%2BD1MXnt1Sht7Y%3D
- (2009) Nat Meth , vol.6 , pp. 503-506
- Taniguchi, K.¹ Kajiyama, T.² Kambara, H.³

26
- 43949143163
- A cautionary note on the use of nested PCR for parasite screening - An example from avian blood parasites
- DOI 10.1645/GE-1286.1
- E Szöllos O Hellgren D Hasselquis 2008 J Parasitol 94 562 564 10.1645/GE-1286.1 (Pubitemid 351706071)
- (2008) Journal of Parasitology , vol.94 , Issue.2 , pp. 562-564
- Szollosi, E.¹ Hellgren, O.² Hasselquist, D.³

27
- 0031940211
- Genetic analysis of long-term Barrett's esophagus epithelial cultures exhibiting cytogenetic and ploidy abnormalities
- DOI 10.1016/S0016-5085(98)70480-9
- MC Polanca-Wessels MT Barrett PC Galipeau KL Rohrer BJ Reid PS Rabinovitch 1998 Gastroenterology 114 295 304 10.1016/S0016-5085(98)70480-9 (Pubitemid 28093536)
- (1998) Gastroenterology , vol.114 , Issue.2 , pp. 295-304
- Palanca-Wessels, M.C.¹ Barrett, M.T.² Galipeau, P.C.³ Rohrer, K.L.⁴ Reid, B.J.⁵ Rabinovitch, P.S.⁶

28
- 0042169024
- Extended lifespan of Barrett's esophagus epithelium transduced with the human telomerase catalytic subunit: A useful in vitro model
- DOI 10.1093/carcin/bgg076
- MCA Palanca-Wessels A Klingelhutz BJ Reid TH Norwood KF Opheim 2003 Carcinogenesis 24 1183 1190 10.1093/carcin/bgg076 1:CAS:528:DC%2BD3sXlvF2rtb0%3D (Pubitemid 36927058)
- (2003) Carcinogenesis , vol.24 , Issue.7 , pp. 1183-1190
- Palanca-Wessels, M.C.A.¹ Klingelhutz, A.² Reid, B.J.³ Norwood, T.H.⁴ Opheim, K.E.⁵ Paulson, T.G.⁶ Feng, Z.⁷ Rabinovitch, P.S.⁸

29
- 54949097443
- Automated vision-based selection and placement of single cells in microwell array formats
- Washington, DC, USA, 23-26 Aug 2008
- Anis YH, Holl MR, Meldrum DR (2008) Automated vision-based selection and placement of single cells in microwell array formats. In: Proc 4th IEEE Conf Automation Science and Engineering, Washington, DC, USA, 23-26 Aug 2008, p 315
- (2008) Proc 4th IEEE Conf Automation Science and Engineering , pp. 315
- Anis, Y.H.¹ Holl, M.R.² Meldrum, D.R.³

30
- 1242296843
- Linear-After-The-Exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis
- DOI 10.1073/pnas.0305476101
- JA Sanchez KE Pierce JE Rice LJ Wangh 2004 Proc Natl Acad Sci USA 101 1933 1938 10.1073/pnas.0305476101 1:CAS:528:DC%2BD2cXhs1Sktbc%3D (Pubitemid 38228993)
- (2004) Proceedings of the National Academy of Sciences of the United States of America , vol.101 , Issue.7 , pp. 1933-1938
- Sanchez, J.A.¹ Pierce, K.E.² Rice, J.E.³ Wangh, L.J.⁴

31
- 20844433243
- Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection
- DOI 10.1073/pnas.0501946102
- KE Pierce JA Sanchez JE Rice LJ Wangh 2005 Proc Natl Acad Sci USA 102 8609 8614 10.1073/pnas.0501946102 1:CAS:528:DC%2BD2MXlsFCgt78%3D (Pubitemid 40862783)
- (2005) Proceedings of the National Academy of Sciences of the United States of America , vol.102 , Issue.24 , pp. 8609-8614
- Pierce, K.E.¹ Sanchez, J.A.² Rice, J.E.³ Wangh, L.J.⁴

32
- 13244265562
- Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
- DOI 10.1186/1472-6750-5-2
- C Hartshorn A Anshelevich LJ Wangh 2005 BMC Biotechnol 5 2 10.1186/1472-6750-5-2 (Pubitemid 40195479)
- (2005) BMC Biotechnology , vol.5 , pp. 2
- Hartshorn, C.¹ Anshelevich, A.² Wangh, L.J.³

33
- 33646362745
- Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR
- DOI 10.1016/j.ab.2006.02.012, PII S000326970600114X
- JJ Salk JA Sanchez KE Peirce JE Rice KC Soares LJ Wangh 2006 Anal Biochem 353 124 132 10.1016/j.ab.2006.02.012 1:CAS:528:DC%2BD28Xks1Wlsr0%3D (Pubitemid 43668947)
- (2006) Analytical Biochemistry , vol.353 , Issue.1 , pp. 124-132
- Salk, J.J.¹ Sanchez, J.A.² Pierce, K.E.³ Rice, J.E.⁴ Soares, K.C.⁵ Wangh, L.J.⁶

34
- 0034727908
- 10.1016/S1566-0702(00)00245-9 1:CAS:528:DC%2BD3MXmtVWgug%3D%3D
- JK Phillips J Lipski 2000 Auton Neurosci 86 1 12 10.1016/S1566-0702(00) 00245-9 1:CAS:528:DC%2BD3MXmtVWgug%3D%3D
- (2000) Auton Neurosci , vol.86 , pp. 1-12
- Phillips, J.K.¹ Lipski, J.²

35
- 50849138472
- 10.1063/1.2969658
- S McGuire C Fisher MR Holl DR Meldrum 2008 Rev Sci Instrum 79 086111 10.1063/1.2969658
- (2008) Rev Sci Instrum , vol.79 , pp. 086111
- McGuire, S.¹ Fisher, C.² Holl, M.R.³ Meldrum, D.R.⁴

36
- 54949099680
- A flexible framework for automation of single cell and cell-to-cell interaction analyses
- Washington, DC, USA, 23-26 Aug 2008
- Nandakumar V, Holl MR, Meldrum DR (2008) A flexible framework for automation of single cell and cell-to-cell interaction analyses. In: Proc 4th IEEE Conf Automation Science and Engineering, Washington, DC, USA, 23-26 Aug 2008, p 424
- (2008) Proc 4th IEEE Conf Automation Science and Engineering , pp. 424
- Nandakumar, V.¹ Holl, M.R.² Meldrum, D.R.³

37
- 62649136506
- 10.1039/b819887j 1:CAS:528:DC%2BD1MXjt1amu70%3D
- SH Chao DR Meldrum 2009 Lab Chip 9 867 869 10.1039/b819887j 1:CAS:528:DC%2BD1MXjt1amu70%3D
- (2009) Lab Chip , vol.9 , pp. 867-869
- Chao, S.H.¹ Meldrum, D.R.²

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.