Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes

Applied and Environmental Microbiology

Volumn 80, Issue 8, 2014, Pages 2468-2477

  Kirmair, Ludwig ^a   Skerra, Arne ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDES; BACTERIA; CELL MEMBRANES; CHROMATOGRAPHY; CIRCULAR DICHROISM SPECTROSCOPY; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; ESTERIFICATION; ESTERS; PLANTS (BOTANY); PURIFICATION; SATURATED FATTY ACIDS; UREA;

ALCOHOL DEHYDROGENASE; BIOTECHNOLOGICAL PRODUCTION; CHARACTERISTIC ABSORPTION; FLAVIN ADENINE DINUCLEOTIDE; PHYSIOLOGICAL ENVIRONMENT; SPECTROPHOTOMETRIC ANALYSIS; SUBSTRATE SPECIFICITY; TEMPERATURE DEPENDENT;

SUBSTRATES;

ALCOHOL; ALDEHYDE; BIOASSAY; BIOCHEMISTRY; BIOTECHNOLOGY; CYTOPLASM; INDUSTRIAL DEVELOPMENT; MEMBRANE; MICROBIAL ACTIVITY; PROTEIN; SUBSTRATE; TEMPERATURE EFFECT;

ALCOHOL DEHYDROGENASE; ALDEHYDE; COENZYME; FLAVINE ADENINE NUCLEOTIDE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; CHEMISTRY; COENZYME; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN DENATURATION; PROTEIN MULTIMERIZATION; PSEUDOMONAS PUTIDA; TEMPERATURE;

ALCOHOL DEHYDROGENASE; ALDEHYDES; CHROMATOGRAPHY, AFFINITY; COENZYMES; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; PROTEIN DENATURATION; PROTEIN MULTIMERIZATION; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84896908827     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.04297-13     Document Type: Article

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