Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants

Applied and Environmental Microbiology

Volumn 72, Issue 6, 2006, Pages 4388-4396

  Ohta, Takeshi ^a,c   Kawabata, Takeshi ^b,d   Nishikawa, Ken ^b   Tani, Akio ^a   Kimbara, Kazuhide ^a   Kawai, Fusako ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

^c EHIME UNIVERSITY   (Japan)

^d NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; CHEMICAL BONDS; ENZYMES; MONOMERS; POLYETHYLENE GLYCOLS; SUBSTRATES; THREE DIMENSIONAL;

AMINO ACID RESIDUES; ELECTRON TRANSFER PATHWAY; POLYETHYLENE GLYCOL DEHYDROGENASE (PEGDH); SPHINGOPYXIS TERRAE;

MICROBIOLOGY;

ADENOSINE DIPHOSPHATE; AMINO ACID; ASPARAGINE; BACTERIAL ENZYME; CHOLINE; CYSTEINE; DISULFIDE; FLAVINE ADENINE NUCLEOTIDE; GLUCOSE; GLUCOSE METHANOL CHOLINE OXIDOREDUCTASE; GLUCOSE OXIDASE; HISTIDINE; MACROGOL; METHANOL; MUTANT PROTEIN; OXIDOREDUCTASE; POLYETHYLENEGLYCOL DEHYDROGENASE; QUERCETIN; UBIDECARENONE; UBIQUINONE; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; CATALYSIS; ENZYME; MOLECULAR ANALYSIS; REACTION KINETICS; THREE-DIMENSIONAL MODELING;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; BACTERIUM MUTANT; BINDING SITE; CATALYSIS; CONSENSUS SEQUENCE; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; MOLECULAR MODEL; NONHUMAN; PENICILLIUM; PENICILLIUM AMAGASAKIENSE; PROTEIN MOTIF; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; SPHINGOMONAS; SPHINGOPYXIS TERRAE; THREE DIMENSIONAL MOLECULAR MODEL; WILD TYPE;

ALCOHOL OXIDOREDUCTASES; ALPHAPROTEOBACTERIA; AMINO ACID SEQUENCE; AMINO ACIDS; BACTERIAL PROTEINS; BASE SEQUENCE; CATALYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDOREDUCTASES; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

ASPERGILLUS NIGER; PENICILLIUM AMAGASAKIENSE; SPHINGOPYXIS TERRAE;

EID: 33745146505     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02174-05     Document Type: Article

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