Universal genetic assay for engineering extracellular protein expression

ACS Synthetic Biology

Volumn 3, Issue 2, 2014, Pages 74-82

  Haitjema, Charles H ^a   Boock, Jason T ^b   Natarajan, Aravind ^a   Dominguez, Miguel A ^c   Gardner, Jeffrey G ^c   Keating, David H ^c   Withers, Sydnor T ^c   Delisa, Matthew P ^a,b  

^a Department of Microbiology Cornell University ^*  (United States)

^b School of Operations Research and Industrial Engineering   (United States)

^c USA   (United States)

Author keywords

bacterial expression; biarsenical FlAsH labeling; protein folding and export; secretion pathway engineering; type II and type III protein secretion; YebF

Indexed keywords

BACTERIAL PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN; SCLEROPROTEIN; SECRETORY PROTEIN; UNCLASSIFIED DRUG; YEBF PROTEIN; 4',5'-BIS(1,3,2-DITHIOARSOLAN-2-YL)FLUORESCEIN; CELLULASE; ESCHERICHIA COLI PROTEIN; FLUORESCEIN DERIVATIVE; ORGANOMETALLIC COMPOUND; TRANSPOSON; YEBF PROTEIN, E COLI;

ARTICLE; BACTERIAL STRAIN; CHEMICAL LABELING; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENETIC MODEL; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SECRETORY PATHWAY; SUPERNATANT; TYPE II SECRETION SYSTEM; TYPE III SECRETION SYSTEM; AMINO ACID SEQUENCE; BIOSYNTHESIS; CELLVIBRIO; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PLASMID; PROTEIN TRANSPORT; TRANSPOSON;

AMINO ACID SEQUENCE; CELLULASES; CELLVIBRIO; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCEINS; GENE EXPRESSION REGULATION; ORGANOMETALLIC COMPOUNDS; PLASMIDS; PROTEIN ENGINEERING; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 84896884825     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb400142b     Document Type: Article

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