Simplex and multiplex real-time PCR assays for the detection of flagellar (H-antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7

Journal of Applied Microbiology

Volumn 109, Issue 5, 2010, Pages 1696-1705

  Madic, J ^a,b   Peytavin De Garam, C ^a   Vingadassalon, N ^a,b   Oswald, E ^c,d,e   Fach, P ^a   Jamet, E ^b   Auvray, F ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b ACTILAIT   (France)

^c UNIVERSITÉ DE TOULOUSE   (France)

^d UNIVERSITÉ PAUL SABATIER   (France)

^e CHU PURPAN   (France)

Author keywords

Detection; eae; EHEC; fliC; Food safety; Rapid methods; Real time PCR

Indexed keywords

ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; POLYMERASE CHAIN REACTION; SIGNAL ENCODING;

DETECTION; EAE; ENTEROHEMORRHAGIC ESCHERICHIA COLI; FLIC; FOOD-SAFETY; PCR ASSAY; RAPID METHOD; REAL-TIME PCR; REAL-TIME PCR ASSAY; SEROTYPES;

ANTIGENS;

BACTERIAL ANTIGEN; H ANTIGEN; INTIMIN; PRIMER DNA; UNCLASSIFIED DRUG;

ALLELE; COLIFORM BACTERIUM; FOOD SAFETY; GENOME; POLYMERASE CHAIN REACTION; REAL TIME; SAMPLING;

ALLELE; ARTICLE; BACTERIAL GENE; CHEESE; CONTROLLED STUDY; ENTEROHEMORRHAGIC ESCHERICHIA COLI; FLAGELLUM; FLIC BACTERIAL GENE; GENE TARGETING; GENETIC VARIABILITY; MOLECULAR PROBE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SEROTYPE; SIMPLEX POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 78650498951     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04798.x     Document Type: Article

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