메뉴 건너뛰기




Volumn 6, Issue 1, 2014, Pages

A microfluidic chip with a U-shaped microstructure array for multicellular spheroid formation, culturing and analysis

Author keywords

drug screening; micro scale bioreactor; microfluidic systems; multicellular spheroids; photoresponsive hydrogel

Indexed keywords

DRUG SCREENING; MICRO FLUIDIC SYSTEM; MICROSTRUCTURE ARRAYS; MULTICELLULAR SPHEROID; PERMSELECTIVE PROPERTIES; PHOTO-RESPONSIVE; POLYETHYLENE GLYCOL DIACRYLATE; THREE DIMENSIONAL CELL CULTURE;

EID: 84896787367     PISSN: 17585082     EISSN: 17585090     Source Type: Journal    
DOI: 10.1088/1758-5082/6/1/015009     Document Type: Article
Times cited : (76)

References (61)
  • 1
    • 0042358985 scopus 로고    scopus 로고
    • Cell culture: Biology's new dimension
    • 10.1038/424870a
    • Abbott A 2003 Cell culture: biology's new dimension Nature 424 870-2
    • (2003) Nature , vol.424 , pp. 870-872
    • Abbott, A.1
  • 2
    • 0030697292 scopus 로고    scopus 로고
    • Three-dimensional cell cultures: From molecular mechanisms to clinical applications
    • 0002-9513
    • Mueller-Klieser W 1997 Three-dimensional cell cultures: from molecular mechanisms to clinical applications Am. J. Physiol. 273 C1109-23
    • (1997) Am. J. Physiol. , vol.273
    • Mueller-Klieser, W.1
  • 3
    • 34648834682 scopus 로고    scopus 로고
    • The third dimension bridges the gap between cell culture and live tissue
    • 10.1038/nrm2236 1471-0080
    • Pampaloni F, Reynaud E G and Stelzer E H 2007 The third dimension bridges the gap between cell culture and live tissue Nature Rev. Mol. Cell. Biol. 8 839-45
    • (2007) Nature Rev. Mol. Cell. Biol. , vol.8 , pp. 839-845
    • Pampaloni, F.1    Reynaud, E.G.2    Stelzer, E.H.3
  • 4
    • 33644529130 scopus 로고    scopus 로고
    • Capturing complex 3D tissue physiology in vitro
    • 10.1038/nrm1858 1471-0080
    • Griffith L G and Swartz M A 2006 Capturing complex 3D tissue physiology in vitro Nature Rev. Mol. Cell. Biol. 7 211-24
    • (2006) Nature Rev. Mol. Cell. Biol. , vol.7 , pp. 211-224
    • Griffith, L.G.1    Swartz, M.A.2
  • 5
    • 0027595948 scopus 로고
    • Tissue engineering
    • 10.1126/science.8493529
    • Langer R and Vacanti J P 1993 Tissue engineering Science 260 920-6
    • (1993) Science , vol.260 , pp. 920-926
    • Langer, R.1    Vacanti, J.P.2
  • 7
    • 84982339248 scopus 로고
    • A study of the mechanics of gastrulation
    • 10.1002/jez.1400950203 0022-104X
    • Holtfreter J 1944 A study of the mechanics of gastrulation J. Exp. Zool. 95 171-212
    • (1944) J. Exp. Zool. , vol.95 , pp. 171-212
    • Holtfreter, J.1
  • 8
    • 0000273795 scopus 로고
    • The dissociation and aggregation of cells from organ rudiments of the early chick embryo
    • 0021-8782
    • Moscona A and Moscona H 1952 The dissociation and aggregation of cells from organ rudiments of the early chick embryo J. Anat. 86 287-301
    • (1952) J. Anat. , vol.86 , pp. 287-301
    • Moscona, A.1    Moscona, H.2
  • 9
    • 60549103259 scopus 로고    scopus 로고
    • Spheroid-based drug screen: Considerations and practical approach
    • 10.1038/nprot.2008.226
    • Friedrich J, Seidel C, Ebner R and Kunz-Schughart L A 2009 Spheroid-based drug screen: considerations and practical approach Nature Protoc. 4 309-24
    • (2009) Nature Protoc. , vol.4 , pp. 309-324
    • Friedrich, J.1    Seidel, C.2    Ebner, R.3    Kunz-Schughart, L.A.4
  • 10
    • 84858723379 scopus 로고    scopus 로고
    • Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation
    • 10.1186/1741-7007-10-29
    • Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D and Eccles S A 2012 Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation BMC Biol. 10 29
    • (2012) BMC Biol. , vol.10 , pp. 29
    • Vinci, M.1    Gowan, S.2    Boxall, F.3    Patterson, L.4    Zimmermann, M.5    Court, W.6    Lomas, C.7    Mendiola, M.8    Hardisson, D.9    Eccles, S.A.10
  • 11
    • 0023093713 scopus 로고
    • Multicellular spheroids. A review on cellular aggregates in cancer research
    • 10.1007/BF00391431 0171-5216
    • Mueller-Klieser W 1987 Multicellular spheroids. A review on cellular aggregates in cancer research J. Cancer Res. Clin. Oncol. 113 101-22
    • (1987) J. Cancer Res. Clin. Oncol. , vol.113 , pp. 101-122
    • Mueller-Klieser, W.1
  • 14
    • 0032508679 scopus 로고    scopus 로고
    • Multicellular spheroids as an in vitro tumor model
    • 10.1016/S0304-3835(98)00198-0
    • Hamilton G 1998 Multicellular spheroids as an in vitro tumor model Cancer Lett. 131 29-34
    • (1998) Cancer Lett. , vol.131 , pp. 29-34
    • Hamilton, G.1
  • 16
    • 84869495994 scopus 로고    scopus 로고
    • Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy
    • 10.1016/j.jconrel.2012.04.045 0168-3659
    • Mehta G, Hsiao A Y, Ingram M, Luker G D and Takayama S 2012 Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy J. Control Release 164 192-204
    • (2012) J. Control Release , vol.164 , pp. 192-204
    • Mehta, G.1    Hsiao, A.Y.2    Ingram, M.3    Luker, G.D.4    Takayama, S.5
  • 17
    • 0031883574 scopus 로고    scopus 로고
    • Multicellular spheroids: A three-dimensional in vitro culture system to study tumour biology
    • 10.1046/j.1365-2613.1998.00051.x 0959-9673
    • Kunz-Schughart L A, Kreutz M and Knuechel R 1998 Multicellular spheroids: a three-dimensional in vitro culture system to study tumour biology Int. J. Exp. Pathol. 79 1-23
    • (1998) Int. J. Exp. Pathol. , vol.79 , pp. 1-23
    • Kunz-Schughart, L.A.1    Kreutz, M.2    Knuechel, R.3
  • 18
    • 16544380146 scopus 로고    scopus 로고
    • The use of 3-D cultures for high-throughput screening: The multicellular spheroid model
    • 10.1177/1087057104265040 1087-0571
    • Kunz-Schughart L A, Freyer J P, Hofstaedter F and Ebner R 2004 The use of 3-D cultures for high-throughput screening: the multicellular spheroid model J. Biomol. Screen. 9 273-85
    • (2004) J. Biomol. Screen. , vol.9 , pp. 273-285
    • Kunz-Schughart, L.A.1    Freyer, J.P.2    Hofstaedter, F.3    Ebner, R.4
  • 19
    • 58749093939 scopus 로고    scopus 로고
    • Recent advances in three-dimensional multicellular spheroid culture for biomedical research
    • 10.1002/biot.200700228
    • Lin R Z and Chang H Y 2008 Recent advances in three-dimensional multicellular spheroid culture for biomedical research Biotechnol. J. 3 1172-84
    • (2008) Biotechnol. J. , vol.3 , pp. 1172-1184
    • Lin, R.Z.1    Chang, H.Y.2
  • 22
    • 77952499476 scopus 로고    scopus 로고
    • Fundamentals of microfluidic cell culture in controlled microenvironments
    • 10.1039/b909900j
    • Young E W and Beebe D J 2010 Fundamentals of microfluidic cell culture in controlled microenvironments Chem. Soc. Rev. 39 1036-48
    • (2010) Chem. Soc. Rev. , vol.39 , pp. 1036-1048
    • Young, E.W.1    Beebe, D.J.2
  • 23
    • 84857944649 scopus 로고    scopus 로고
    • Engineers are from PDMS-land, biologists are from polystyrenia
    • 10.1039/c2lc20982a
    • Berthier E, Young E W and Beebe D 2012 Engineers are from PDMS-land, biologists are from polystyrenia Lab Chip 12 1224-37
    • (2012) Lab Chip , vol.12 , pp. 1224-1237
    • Berthier, E.1    Young, E.W.2    Beebe, D.3
  • 24
    • 81355146382 scopus 로고    scopus 로고
    • From 3D cell culture to organs-on-chips
    • 10.1016/j.tcb.2011.09.005
    • Huh D, Hamilton G A and Ingber D E 2011 From 3D cell culture to organs-on-chips Trends Cell Biol. 21 745-54
    • (2011) Trends Cell Biol. , vol.21 , pp. 745-754
    • Huh, D.1    Hamilton, G.A.2    Ingber, D.E.3
  • 27
    • 80052351691 scopus 로고    scopus 로고
    • Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model
    • 10.1016/j.biomaterials.2011.07.028 0142-9612
    • Wong S F, No da Y, Choi Y Y, Kim D S, Chung B G and Lee S H 2011 Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model Biomaterials 32 8087-96
    • (2011) Biomaterials , vol.32 , pp. 8087-8096
    • Wong, S.F.1    No Da, Y.2    Choi, Y.Y.3    Kim, D.S.4    Chung, B.G.5    Lee, S.H.6
  • 29
    • 77957682629 scopus 로고    scopus 로고
    • Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval
    • 10.1039/c0lc00005a
    • Kang E, Choi Y Y, Jun Y, Chung B G and Lee S H 2010 Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval Lab Chip 10 2651-4
    • (2010) Lab Chip , vol.10 , pp. 2651-2654
    • Kang, E.1    Choi, Y.Y.2    Jun, Y.3    Chung, B.G.4    Lee, S.H.5
  • 30
    • 36849072481 scopus 로고    scopus 로고
    • Functional three-dimensional HepG2 aggregate cultures generated from an ultrasound trap: Comparison with HepG2 spheroids
    • 10.1002/jcb.21345
    • Liu J, Kuznetsova L A, Edwards G O, Xu J, Ma M, Purcell W M, Jackson S K and Coakley W T 2007 Functional three-dimensional HepG2 aggregate cultures generated from an ultrasound trap: comparison with HepG2 spheroids J. Cell. Biochem. 102 1180-9
    • (2007) J. Cell. Biochem. , vol.102 , pp. 1180-1189
    • Liu, J.1    Kuznetsova, L.A.2    Edwards, G.O.3    Xu, J.4    Ma, M.5    Purcell, W.M.6    Jackson, S.K.7    Coakley, W.T.8
  • 31
    • 35048832907 scopus 로고    scopus 로고
    • Tissue engineering with electric fields: Immobilization of mammalian cells in multilayer aggregates using dielectrophoresis
    • 10.1002/bit.21416
    • Sebastian A, Buckle A M and Markx G H 2007 Tissue engineering with electric fields: immobilization of mammalian cells in multilayer aggregates using dielectrophoresis Biotechnol. Bioeng. 98 694-700
    • (2007) Biotechnol. Bioeng. , vol.98 , pp. 694-700
    • Sebastian, A.1    Buckle, A.M.2    Markx, G.H.3
  • 32
    • 40349103280 scopus 로고    scopus 로고
    • Microfluidic self-assembly of tumor spheroids for anticancer drug discovery
    • 10.1007/s10544-007-9125-8
    • Wu L Y, Di Carlo D and Lee L P 2008 Microfluidic self-assembly of tumor spheroids for anticancer drug discovery Biomed. Microdevices 10 197-202
    • (2008) Biomed. Microdevices , vol.10 , pp. 197-202
    • Wu, L.Y.1    Di Carlo, D.2    Lee, L.P.3
  • 33
    • 77956102001 scopus 로고    scopus 로고
    • Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing
    • 10.1039/c004590j
    • Yu L, Chen M C and Cheung K C 2010 Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing Lab Chip 10 2424-32
    • (2010) Lab Chip , vol.10 , pp. 2424-2432
    • Yu, L.1    Chen, M.C.2    Cheung, K.C.3
  • 34
    • 78650084562 scopus 로고    scopus 로고
    • A multicellular spheroid formation and extraction chip using removable cell trapping barriers
    • 10.1039/c0lc00134a
    • Jin H J, Cho Y H, Gu J M, Kim J and Oh Y S 2011 A multicellular spheroid formation and extraction chip using removable cell trapping barriers Lab Chip 11 115-9
    • (2011) Lab Chip , vol.11 , pp. 115-119
    • Jin, H.J.1    Cho, Y.H.2    Gu, J.M.3    Kim, J.4    Oh, Y.S.5
  • 36
    • 84859795101 scopus 로고    scopus 로고
    • Dynamic trapping and high-throughput patterning of cells using pneumatic microstructures in an integrated microfluidic device
    • 10.1039/c2lc00034b
    • Liu W, Li L, Wang J C, Tu Q, Ren L, Wang Y and Wang J 2012 Dynamic trapping and high-throughput patterning of cells using pneumatic microstructures in an integrated microfluidic device Lab Chip 12 1702-9
    • (2012) Lab Chip , vol.12 , pp. 1702-1709
    • Liu, W.1    Li, L.2    Wang, J.C.3    Tu, Q.4    Ren, L.5    Wang, Y.6    Wang, J.7
  • 37
    • 0029007273 scopus 로고
    • Biomaterials in tissue engineering
    • 10.1038/nbt0695-565 1087-0156
    • Hubbell J A 1995 Biomaterials in tissue engineering Nature Biotechnol. 13 565-76
    • (1995) Nature Biotechnol. , vol.13 , pp. 565-576
    • Hubbell, J.A.1
  • 38
    • 67650169752 scopus 로고    scopus 로고
    • Hydrogels as extracellular matrix mimics for 3D cell culture
    • 10.1002/bit.22361
    • Tibbitt M W and Anseth K S 2009 Hydrogels as extracellular matrix mimics for 3D cell culture Biotechnol. Bioeng. 103 655-63
    • (2009) Biotechnol. Bioeng. , vol.103 , pp. 655-663
    • Tibbitt, M.W.1    Anseth, K.S.2
  • 39
    • 34748902324 scopus 로고    scopus 로고
    • Microengineered hydrogels for tissue engineering
    • 10.1016/j.biomaterials.2007.07.021 0142-9612
    • Khademhosseini A and Langer R 2007 Microengineered hydrogels for tissue engineering Biomaterials 28 5087-92
    • (2007) Biomaterials , vol.28 , pp. 5087-5092
    • Khademhosseini, A.1    Langer, R.2
  • 40
    • 35348874191 scopus 로고    scopus 로고
    • Review: Photopolymerizable and degradable biomaterials for tissue engineering applications
    • 10.1089/ten.2007.0093 1076-3279
    • Ifkovits J L and Burdick J A 2007 Review: photopolymerizable and degradable biomaterials for tissue engineering applications Tissue Eng. 13 2369-85
    • (2007) Tissue Eng. , vol.13 , pp. 2369-2385
    • Ifkovits, J.L.1    Burdick, J.A.2
  • 41
    • 33749579075 scopus 로고    scopus 로고
    • Assessment of hepatocellular function within PEG hydrogels
    • 10.1016/j.biomaterials.2006.08.043 0142-9612
    • Underhill G H, Chen A A, Albrecht D R and Bhatia S N 2007 Assessment of hepatocellular function within PEG hydrogels Biomaterials 28 256-70
    • (2007) Biomaterials , vol.28 , pp. 256-270
    • Underhill, G.H.1    Chen, A.A.2    Albrecht, D.R.3    Bhatia, S.N.4
  • 42
    • 77953626607 scopus 로고    scopus 로고
    • Injectable biodegradable hydrogels
    • 10.1002/mabi.200900402
    • Nguyen M K and Lee D S 2010 Injectable biodegradable hydrogels Macromol. Biosci. 10 563-79
    • (2010) Macromol. Biosci. , vol.10 , pp. 563-579
    • Nguyen, M.K.1    Lee, D.S.2
  • 44
    • 30944441438 scopus 로고    scopus 로고
    • Photolithographic patterning of polyethylene glycol hydrogels
    • 10.1016/j.biomaterials.2005.11.045 0142-9612
    • Hahn M S, Taite L J, Moon J J, Rowland M C, Ruffino K A and West J L 2006 Photolithographic patterning of polyethylene glycol hydrogels Biomaterials 27 2519-24
    • (2006) Biomaterials , vol.27 , pp. 2519-2524
    • Hahn, M.S.1    Taite, L.J.2    Moon, J.J.3    Rowland, M.C.4    Ruffino, K.A.5    West, J.L.6
  • 45
    • 47749117234 scopus 로고    scopus 로고
    • Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
    • 10.1073/pnas.0801866105 0027-8424
    • Du Y, Lo E, Ali S and Khademhosseini A 2008 Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs Proc. Natl Acad. Sci. USA 105 9522-7
    • (2008) Proc. Natl Acad. Sci. USA , vol.105 , pp. 9522-9527
    • Du, Y.1    Lo, E.2    Ali, S.3    Khademhosseini, A.4
  • 46
    • 33646475253 scopus 로고    scopus 로고
    • Continuous-flow lithography for high-throughput microparticle synthesis
    • 10.1038/nmat1617 1476-1122
    • Dendukuri D, Pregibon D C, Collins J, Hatton T A and Doyle P S 2006 Continuous-flow lithography for high-throughput microparticle synthesis Nature Mater. 5 365-9
    • (2006) Nature Mater. , vol.5 , pp. 365-369
    • Dendukuri, D.1    Pregibon, D.C.2    Collins, J.3    Hatton, T.A.4    Doyle, P.S.5
  • 47
    • 67649366621 scopus 로고    scopus 로고
    • Three-dimensional fabrication of heterogeneous microstructures using soft membrane deformation and optofluidic maskless lithography
    • 10.1039/b819999j
    • Lee S A, Chung S E, Park W, Lee S H and Kwon S 2009 Three-dimensional fabrication of heterogeneous microstructures using soft membrane deformation and optofluidic maskless lithography Lab Chip 9 1670-5
    • (2009) Lab Chip , vol.9 , pp. 1670-1675
    • Lee, S.A.1    Chung, S.E.2    Park, W.3    Lee, S.H.4    Kwon, S.5
  • 49
    • 79961037261 scopus 로고    scopus 로고
    • A simple cell patterning method using magnetic particle-containing photosensitive poly (ethylene glycol) hydrogel blocks: A technical note
    • 10.1089/ten.tec.2010.0690 1076-3279 C
    • Fu C Y, Lin C Y, Chu W C and Chang H Y 2011 A simple cell patterning method using magnetic particle-containing photosensitive poly (ethylene glycol) hydrogel blocks: a technical note Tissue Eng. C 17 871-7
    • (2011) Tissue Eng. , vol.17 , pp. 871-877
    • Fu, C.Y.1    Lin, C.Y.2    Chu, W.C.3    Chang, H.Y.4
  • 50
    • 78449290991 scopus 로고    scopus 로고
    • Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs
    • 10.1111/j.1349-7006.2010.01723.x
    • Ho W J, Pham E A, Kim J W, Ng C W, Kim J H, Kamei D T and Wu B M 2010 Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs Cancer Sci. 101 2637-43
    • (2010) Cancer Sci. , vol.101 , pp. 2637-2643
    • Ho, W.J.1    Pham, E.A.2    Kim, J.W.3    Ng, C.W.4    Kim, J.H.5    Kamei, D.T.6    Wu, B.M.7
  • 51
    • 79954752177 scopus 로고    scopus 로고
    • Enhancement of drug efflux activity via MDR1 protein by spheroid culture of human hepatic cancer cells
    • 10.1016/j.jbiosc.2011.01.006
    • Oshikata A, Matsushita T and Ueoka R 2011 Enhancement of drug efflux activity via MDR1 protein by spheroid culture of human hepatic cancer cells J. Biosci. Bioeng. 111 590-3
    • (2011) J. Biosci. Bioeng. , vol.111 , pp. 590-593
    • Oshikata, A.1    Matsushita, T.2    Ueoka, R.3
  • 52
    • 39649115200 scopus 로고    scopus 로고
    • Partitioning microfluidic channels with hydrogel to construct tunable 3-D cellular microenvironments
    • 10.1016/j.biomaterials.2007.12.044 0142-9612
    • Wong A P, Perez-Castillejos R, Christopher Love J and Whitesides G M 2008 Partitioning microfluidic channels with hydrogel to construct tunable 3-D cellular microenvironments Biomaterials 29 1853-61
    • (2008) Biomaterials , vol.29 , pp. 1853-1861
    • Wong, A.P.1    Perez-Castillejos, R.2    Christopher Love, J.3    Whitesides, G.M.4
  • 54
    • 84862157431 scopus 로고    scopus 로고
    • Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
    • 10.1038/nprot.2012.051
    • Shin Y, Han S, Jeon J S, Yamamoto K, Zervantonakis I K, Sudo R, Kamm R D and Chung S 2012 Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels Nature Protoc. 7 1247-59
    • (2012) Nature Protoc. , vol.7 , pp. 1247-1259
    • Shin, Y.1    Han, S.2    Jeon, J.S.3    Yamamoto, K.4    Zervantonakis, I.K.5    Sudo, R.6    Kamm, R.D.7    Chung, S.8
  • 55
    • 0034611669 scopus 로고    scopus 로고
    • Functional hydrogel structures for autonomous flow control inside microfluidic channels
    • 10.1038/35007047
    • Beebe D J, Moore J S, Bauer J M, Yu Q, Liu R H, Devadoss C and Jo B H 2000 Functional hydrogel structures for autonomous flow control inside microfluidic channels Nature 404 588-90
    • (2000) Nature , vol.404 , pp. 588-590
    • Beebe, D.J.1    Moore, J.S.2    Bauer, J.M.3    Yu, Q.4    Liu, R.H.5    Devadoss, C.6    Jo, B.H.7
  • 56
    • 0024281372 scopus 로고
    • Cell and environment interactions in tumor microregions: The multicell spheroid model
    • 10.1126/science.2451290
    • Sutherland R M 1988 Cell and environment interactions in tumor microregions: the multicell spheroid model Science 240 177-84
    • (1988) Science , vol.240 , pp. 177-184
    • Sutherland, R.M.1
  • 57
    • 0028361447 scopus 로고
    • Drug and radiation resistance in spheroids: Cell contact and kinetics
    • 10.1007/BF00689632 0167-7659
    • Olive P L and Durand R E 1994 Drug and radiation resistance in spheroids: cell contact and kinetics Cancer Metastasis Rev. 13 121-38
    • (1994) Cancer Metastasis Rev. , vol.13 , pp. 121-138
    • Olive, P.L.1    Durand, R.E.2
  • 59
    • 0034328531 scopus 로고    scopus 로고
    • Tumor biology and experimental therapeutics
    • 10.1016/S1040-8428(00)00082-2 1040-8428
    • Mueller-Klieser W 2000 Tumor biology and experimental therapeutics Crit. Rev. Oncol. Hematol. 36 123-39
    • (2000) Crit. Rev. Oncol. Hematol. , vol.36 , pp. 123-139
    • Mueller-Klieser, W.1
  • 60
    • 3042667195 scopus 로고    scopus 로고
    • Three-dimensional in vitro tissue culture models of breast cancer - A review
    • 10.1023/B:BREA.0000025418.88785.2b 0167-6806
    • Kim J B, Stein R and O'Hare M J 2004 Three-dimensional in vitro tissue culture models of breast cancer - a review Breast Cancer Res. Treat. 85 281-91
    • (2004) Breast Cancer Res. Treat. , vol.85 , pp. 281-291
    • Kim, J.B.1    Stein, R.2    O'Hare, M.J.3
  • 61
    • 78751560396 scopus 로고    scopus 로고
    • High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array
    • 10.1039/c0an00609b
    • Tung Y C, Hsiao A Y, Allen S G, Torisawa Y S, Ho M and Takayama S 2011 High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array Analyst 136 473-8
    • (2011) Analyst , vol.136 , pp. 473-478
    • Tung, Y.C.1    Hsiao, A.Y.2    Allen, S.G.3    Torisawa, Y.S.4    Ho, M.5    Takayama, S.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.