High-throughput screening for the development of a monoclonal antibody affinity precipitation step using ELP-z stimuli responsive biopolymers

Biotechnology and Bioengineering

Volumn 110, Issue 10, 2013, Pages 2664-2676

  Sheth, Rahul D ^a   Madan, Bhawna ^b   Chen, Wilfred ^b   Cramer, Steven M ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

Affinity precipitation; Elastin like polypeptides; High throughput screening; Monoclonal antibodies

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BIOPOLYMERS; MONOCLONAL ANTIBODIES;

AFFINITY PRECIPITATION; ELASTIN-LIKE POLYPEPTIDES; HIGH-THROUGHPUT SCREENING; MONOCLONAL ANTIBODIES (MAB); MULTIDIMENSIONAL HIGH-THROUGHPUT SCREENINGS; PRECIPITATION EFFICIENCY; ROOM-TEMPERATURE OPERATION; STIMULI RESPONSIVE BIOPOLYMERS;

AGGREGATES;

BIOPOLYMER; ELASTIN LIKE POLYPEPTIDE Z; MONOCLONAL ANTIBODY; POLYPEPTIDE; SODIUM SULFATE; UNCLASSIFIED DRUG; ELASTIN; SULFATE;

ARTICLE; HIGH THROUGHPUT SCREENING; PH; TEMPERATURE; AFFINITY CHROMATOGRAPHY; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; PRECIPITATION; PROCEDURES; AFFINITY PRECIPITATION; ELASTIN-LIKE POLYPEPTIDES; HIGH-THROUGHPUT SCREENING; METHODOLOGY; MONOCLONAL ANTIBODIES;

ANTIBODIES, MONOCLONAL; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, AFFINITY; ELASTIN; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN-ION CONCENTRATION; SULFATES; TEMPERATURE;

AFFINITY PRECIPITATION; ELASTIN-LIKE POLYPEPTIDES; HIGH-THROUGHPUT SCREENING; MONOCLONAL ANTIBODIES;

EID: 84896413109     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24945     Document Type: Article

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