One-step metal-affinity purification of histidine-tagged proteins by temperature-triggered precipitation

Biotechnology and Bioengineering

Volumn 82, Issue 5, 2003, Pages 605-611

  Stiborova, Hana ^a   Kostal, Jan ^a   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

Author keywords

Elastin; His tag; Metal affinity; Protein purification

Indexed keywords

ALDEHYDES; PRECIPITATION (CHEMICAL); PURIFICATION; SYNTHESIS (CHEMICAL); THERMAL EFFECTS;

METAL-AFFINITY PURIFICATION;

PROTEINS;

ALDEHYDE; BETA GALACTOSIDASE; CHLORAMPHENICOL ACETYLTRANSFERASE; COORDINATION COMPOUND; ELASTIN; ENZYME; HISTIDINE TAGGED PROTEIN; IMIDAZOLE 2 CARBOXALDEHYDE; LYSINE; PENTAPEPTIDE; PROTEIN; UNCLASSIFIED DRUG; VALYLPROLYLGLYCLVALYLGLYCINAMIDE;

ADDITION REACTION; ARTICLE; BINDING AFFINITY; ENZYME BINDING; ENZYME RELEASE; LIGAND BINDING; METAL BINDING; OBSERVATION; POLYMERIZATION; PRECIPITATION; PRODUCT RECOVERY; PROTEIN BINDING; PROTEIN MODIFICATION; PURIFICATION; RECYCLING; TEMPERATURE MEASUREMENT; TEMPERATURE TRIGGERED PRECIPITATION; TRANSITION TEMPERATURE;

AFFINITY LABELS; BETA-GALACTOSIDASE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHROMATOGRAPHY, AFFINITY; ELASTIN; ESCHERICHIA COLI; HISTIDINE; METALS; NICKEL; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

EID: 0037420917     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10609     Document Type: Article

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