High-throughput screening techniques for rapid PEG-based precipitation of IgG4 mAb from clarified cell culture supernatant

Biotechnology Progress

Volumn 26, Issue 3, 2010, Pages 697-705

  Knevelman, Carol ^a,b   Davies, Jim ^b   Allen, Lee ^b   Titchener Hooker, Nigel J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b LONZA AG   (Switzerland)

Author keywords

High throughput screening; IgG4; Micro scale; Microwell; PEG; Precipitation; Purification; Recovery

Indexed keywords

HIGH-THROUGHPUT SCREENING; MICRO WELLS; MICRO-SCALE; MICRO-SCALES; MICROWELL;

BIOLOGICAL MATERIALS; CELL CULTURE; CONCENTRATION (PROCESS); DESIGN OF EXPERIMENTS; IDENTIFICATION (CONTROL SYSTEMS); MICROCHANNELS; MONOCLONAL ANTIBODIES; OPTIMIZATION; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PURIFICATION;

THROUGHPUT;

IMMUNOGLOBULIN G; MACROGOL DERIVATIVE; MONOCLONAL ANTIBODY;

ARTICLE; CHEMISTRY; CULTURE TECHNIQUE; HIGH THROUGHPUT SCREENING; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; SOLUBILITY; STATISTICAL MODEL; PROCEDURES;

ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CHEMICAL PRECIPITATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; MODELS, STATISTICAL; POLYETHYLENE GLYCOLS; SOLUBILITY;

EID: 77954733862     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.357     Document Type: Article

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