Dimerization of flavivirus NS4B protein

Journal of Virology

Volumn 88, Issue 6, 2014, Pages 3379-3391

  Zou, Jing ^a,b,e   Xie, Xuping ^b   Lee, Le Tian ^b,c   Chandrasekaran, Ramya ^c   Reynaud, Aline ^c   Yap, Lijian ^c   Wang, Qing Yin ^b   Dong, Hongping ^b   Kang, Congbao ^d   Yuan, Zhiming ^a   Lescar, Julien ^c   Shi, Pei Yong ^b  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b Novartis Institute for Tropical Diseases   (Singapore)

^c NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^d Agency for Science   (Singapore)

^e UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; NONSTRUCTURAL PROTEIN 4B; VIRUS RNA;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHEMICAL MUTAGENESIS; CONTROLLED STUDY; CROSS LINKING; CYTOSOLIC LOOP; DENGUE VIRUS 2; DIMERIZATION; ESCHERICHIA COLI; GEL FILTRATION; HUMAN; HUMAN CELL; IN VITRO STUDY; LIGHT SCATTERING; MEMBRANE STRUCTURE; MOSQUITO; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; REPLICON; VIRUS GENOME; VIRUS REPLICATION; WEST NILE FLAVIVIRUS; WILD TYPE;

AMINO ACID MOTIFS; DENGUE; DENGUE VIRUS; DIMERIZATION; HUMANS; VIRAL NONSTRUCTURAL PROTEINS; WEST NILE FEVER; WEST NILE VIRUS;

EID: 84894587388     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.02782-13     Document Type: Article

References (38)

1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI. 2013. The global distribution and burden of dengue. Nature 496:504-507. http://dx.doi.org/10.1038/nature12060.
2. Sabchareon A, Wallace D, Sirivichayakul C, Limkittikul K, Chanthavanich P, Suvannadabba S, Jiwariyavej V, Dulyachai W, Pengsaa K, Wartel TA, Moureau A, Saville M, Bouckenooghe A, Viviani S, Tornieporth NG, Lang J. 2012. Protective efficacy of the recombinant, liveattenuated, CYDtetravalent dengue vaccine in Thai schoolchildren: a randomised, controlled phase 2b trial. Lancet 380:1559-1567. http://dx.doi.org/10.1016/S0140-6736(12)61428-7.
3. Lindenbach BD, Thiel H-J, Rice CM. 2007. Flaviviridae: the viruses and their replication, p 1101-1152. In Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (ed), Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA.
4. Munoz-Jordan JL, Sanchez-Burgos GG, Laurent-Rolle M, Garcia-Sastre A. 2003. Inhibition of interferon signaling by dengue virus. Proc. Natl. Acad. Sci. U.S.A. 100:14333-14338. http://dx.doi.org/10.1073/pnas.2335168100.
5. Morrison J, Aguirre S, Fernandez-Sesma A. 2012. Innate immunity evasion by dengue virus. Viruses 4:397-413. http://dx.doi.org/10.3390/v4030397.
6. Warrener P, Tamura JK, Collett MS. 1993. RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria. J. Virol. 67:989-996.
7. Wengler G, Czaya G, Farber PM, Hegemann JH. 1991. In vitro synthesis of West Nile virus proteins indicates that the amino-terminal segment of the NS3 protein contains the active centre of the protease which cleaves the viral polyprotein after multiple basic amino acids. J. Gen. Virol. 72: 851-858. http://dx.doi.org/10.1099/0022-1317-72-4-851.
8. Li H, Clum S, You S, Ebner KE, Padmanabhan R. 1999. The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids. J. Virol. 73:3108-3116.
9. Egloff MP, Benarroch D, Selisko B, Romette JL, Canard B. 2002. An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. EMBO J. 21:2757-2768. http://dx.doi.org/10.1093/emboj/21.11.2757.
10. Ray D, Shah A, Tilgner M, Guo Y, Zhao Y, Dong H, Deas T, Zhou Y, Li H, Shi P-Y. 2006. West Nile virus 5'-cap structure is formed by sequential guanine N-7 and ribose 2'-O methylations by nonstructural protein 5. J. Virol. 80:8362-8370. http://dx.doi.org/10.1128/JVI.00814-06.
11. Zhou Y, Ray D, Zhao Y, Dong H, Ren S, Li Z, Guo Y, Bernard K, Shi P-Y, Li H. 2007. Structure and function of flavivirus NS5 methyltransferase. J. Virol. 81:3891-3903. http://dx.doi.org/10.1128/JVI.02704-06.
12. Dong H, Chang DC, Hua MH, Lim SP, Chionh YH, Hia F, Lee YH, Kukkaro P, Lok SM, Dedon PC, Shi PY. 2012. 2'-O methylation of internal adenosine by flavivirus NS5 methyltransferase. PLoS Pathog. 8:e1002642. http://dx.doi.org/10.1371/journal.ppat.1002642.
13. Ackermann M, Padmanabhan R. 2001. De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase. J. Biol. Chem. 276: 39926-39937. http://dx.doi.org/10.1074/jbc.M104248200.
14. Leung JY, Pijlman GP, Kondratieva N, Hyde J, Mackenzie JM, Khromykh AA. 2008. Role of nonstructural protein NS2A in flavivirus assembly. J. Virol. 82:4731-4741. http://dx.doi.org/10.1128/JVI.00002-08.
15. Xie X, Gayen S, Kang C, Yuan Z, Shi PY. 2013. Membrane topology and function of dengue virus NS2A protein. J. Virol. 87:4609-4622. http://dx.doi.org/10.1128/JVI.02424-12.
16. Kummerer BM, Rice CM. 2002. Mutations in the yellow fever virus nonstructural protein NS2A selectively block production of infectious particles. J. Virol. 76:4773-4784. http://dx.doi.org/10.1128/JVI.76.10.4773-4784.2002.
17. Miller S, Sparacio S, Bartenschlager R. 2006. Subcellular localization and membrane topology of the dengue virus type 2 non-structural protein 4B. J. Biol. Chem. 281:8854-8863. http://dx.doi.org/10.1074/jbc.M512697200.
18. Roosendaal J, Westaway EG, Khromykh A, Mackenzie JM. 2006. Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein. J. Virol. 80:4623-4632. http://dx.doi.org/10.1128/JVI.80.9.4623-4632.2006.
19. Munoz-Jordan JL, Laurent-Rolle M, Ashour J, Martinez-Sobrido L, Ashok M, Lipkin WI, Garcia-Sastre A. 2005. Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses. J. Virol. 79:8004-8013. http://dx.doi.org/10.1128/JVI.79.13.8004-8013.2005.
20. Kakumani PK, Ponia SS, Rajgokul KS, Sood V, Chinnappan M, Banerjea AC, Medigeshi GR, Malhotra P, Mukherjee SK, Bhatnagar RK. 2013. Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor. J. Virol. 87:8870-8883. http://dx.doi.org/10.1128/JVI.02774-12.
21. Umareddy I, Chao A, Sampath A, Gu F, Vasudevan SG. 2006. Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA. J. Gen. Virol. 87:2605-2614. http://dx.doi.org/10.1099/vir.0.81844-0.
22. Youn S, Li T, McCune BT, Edeling MA, Fremont DH, Cristea IM, Diamond MS. 2012. Evidence for a genetic and physical interaction between nonstructural proteins NS1 and NS4B that modulates replication of West Nile virus. J. Virol. 86:7360-7371. http://dx.doi.org/10.1128/JVI.00157-12.
23. Xie X, Wang QY, Xu HY, Qing M, Kramer L, Yuan Z, Shi PY. 2011. Inhibition of dengue virus by targeting viral NS4B protein. J. Virol. 85: 11183-11195. http://dx.doi.org/10.1128/JVI.05468-11.
24. Patkar CG, Larsen M, Owston M, Smith JL, Kuhn RJ. 2009. Identification of inhibitors of yellow fever virus replication using a replicon-based high-throughput assay. Antimicrob. Agents Chemother. 53:4103-4114. http://dx.doi.org/10.1128/AAC.00074-09.
25. Zou G, Chen YL, Dong H, Lim CC, Yap LJ, Yau YH, Shochat SG, Lescar J, Shi PY. 2011. Functional analysis of two cavities in flavivirus NS5 polymerase. J. Biol. Chem. 286:14362-14372. http://dx.doi.org/10.1074/jbc.M110.214189.
26. Xie X, Zou J, Wang Q-Y, Noble CG, Lescar J, Shi P-Y. 2014. Generation and characterization of mouse monoclonal antibodies against NS4B protein of dengue virus. Virology 450-451:250-257. http://dx.doi.org/10.1016/j.virol.2013.12.025.
27. Lorenz H, Hailey DW, Wunder C, Lippincott-Schwartz J. 2006. The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology. Nat. Protoc. 1:276-279. http://dx.doi.org/10.1038/nprot.2006.42.
28. Yu GY, Lee KJ, Gao L, Lai MM. 2006. Palmitoylation and polymerization of hepatitis C virus NS4B protein. J. Virol. 80:6013-6023. http://dx.doi.org/10.1128/JVI.00053-06.
29. Ng CY, Gu F, Phong WY, Chen YL, Lim SP, Davidson A, Vasudevan SG. 2007. Construction and characterization of a stable subgenomic dengue virus type 2 replicon system for antiviral compound and siRNA testing. Antiviral Res. 76:222-231. http://dx.doi.org/10.1016/j.antiviral.2007.06.007.
30. Lund S, Orlowski S, de Foresta B, Champeil P, le Maire M, Moller JV. 1989. Detergent structure and associated lipid as determinants in the stabilization of solubilized Ca2+-ATPase from sarcoplasmic reticulum. J. Biol. Chem. 264:4907-4915.
31. Berger BW, Garcia RY, Lenhoff AM, Kaler EW, Robinson CR. 2005. Relating surfactant properties to activity and solubilization of the human adenosine a3 receptor. Biophys. J. 89:452-464. http://dx.doi.org/10.1529/biophysj.104.051417.
32. Zou G, Zhang B, Lim PY, Yuan Z, Bernard KA, Shi PY. 2009. Exclusion of West Nile virus superinfection through RNA replication. J. Virol. 83: 11765-11776. http://dx.doi.org/10.1128/JVI.01205-09.
33. Mukhopadhyay S, Kuhn RJ, Rossmann MG. 2005. A structural perspective of the flavivirus life cycle. Nat. Rev. Microbiol. 3:13-22. http://dx.doi.org/10.1038/nrmicro1067.
34. Lescar J, Canard B. 2009. RNA-dependent RNA polymerases from flaviviruses and Picornaviridae. Curr. Opin. Struct. Biol. 19:759-767. http://dx.doi.org/10.1016/j.sbi.2009.10.011.
35. Noble CG, Shi PY. 2012. Structural biology of dengue virus enzymes: towards rational design of therapeutics. Antiviral Res. 96:115-126. http://dx.doi.org/10.1016/j.antiviral.2012.09.007.
36. Mackenzie JM, Khromykh AA, Jones MK, Westaway EG. 1998. Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A. Virology 245:203-215. http://dx.doi.org/10.1006/viro.1998.9156.
37. Miller S, Kastner S, Krijnse-Locker J, Buhler S, Bartenschlager R. 2007. The non-structural protein 4A of dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner. J. Biol. Chem. 282:8873-8882. http://dx.doi.org/10.1074/jbc.M609919200.
38. van Cleef KW, Overheul GJ, Thomassen MC, Kaptein SJ, Davidson AD, Jacobs M, Neyts J, van Kuppeveld FJ, van Rij RP. 2013. Identification of a new dengue virus inhibitor that targets the viral NS4B protein and restricts genomic RNA replication. Antiviral Res. 99:165-171. http://dx.doi.org/10.1016/j.antiviral.2013.05.011.