An RNA cap (nucleoside-2′-O-)-methyltransferase in the flavivirus RNA polymerase NS5: Crystal structure and functional characterization

EMBO Journal

Volumn 21, Issue 11, 2002, Pages 2757-2768

  Egloff, Marie Pierre ^a   Benarroch, Delphine ^a   Selisko, Barbara ^a   Romette, Jean Louis ^a   Canard, Bruno ^a  

^a Centre National de la Recherche Scientifique   (France)

Author keywords

Crystal structure; Flavivirus; GTP; RNA capping; RNA polymerase

Indexed keywords

(NUCLEOSIDE 2' O )METHYLTRANSFERASE; CAPPED RNA; GUANOSINE TRIPHOSPHATE DERIVATIVE; METHYLTRANSFERASE; NUCLEOSIDE; RNA DIRECTED RNA POLYMERASE; RNA METHYLTRANSFERASE; RNA POLYMERASE; S ADENOSYLHOMOCYSTEINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; DENGUE VIRUS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FLAVIVIRUS; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STRUCTURE; RNA ANALYSIS; RNA STRUCTURE; RNA TRANSCRIPTION;

AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DOSE-RESPONSE RELATIONSHIP, DRUG; GUANOSINE TRIPHOSPHATE; METHYLTRANSFERASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEOTIDES; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RNA; TIME FACTORS; VIRAL NONSTRUCTURAL PROTEINS;

DENGUE VIRUS; DENGUE VIRUS TYPE 2; FLAVIVIRUS;

EID: 0037013858     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.11.2757     Document Type: Article

References (57)

1. Reaction in alphavirus mRNA capping: Formation of a covalent complex of nonstructural protein nsP1 with 7-methyl-GMP
2. Isolation of West Nile virus from mosquitoes, crows and a Cooper's hawk in Connecticut
3. mRNA(nucleoside-2′-)-methyltransferase from vaccinia virus. Characteristics and substrate specificity
4. Viral and cellular enzymes involved in synthesis of mRNA cap structure
5. The free R value: A novel statistical quantity for assessing the accuracy of crystal structures
6. Crystallography and NMR system: A new software suite for macromolecular structure determination
7. RNA methylation under heat shock control
8. Reassignment of specificities of two cap methyltransferase domains in the reovirus lambda 2 protein
9. mRNA:guanine-N7 cap methyltransferases: Identification of novel members of the family, evolutionary analysis, homology modeling and analysis of sequence-structure-function relationships
10. Crystal structure of ErmC′, an rRNA methyltransferase which mediates antibiotic resistance in bacteria
11. The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23S ribosomal RNA methyltransferase
12. The CCP4 suite: Programs for protein crystallography
13. Flavivirus genome organization, expression and replication
14. Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme
15. DM: An automated procedure for phase improvement by density modification
16. Crystal structure of the chemotaxis receptor methyltransferase CheR suggests a conserved structural motif for binding S-adenosylmethionine
17. West Nile Watch
18. Structure and evolution of AdoMet-dependent methyltransferases
19. The 37-amino-acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site
20. An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of helicase activity
21. Crystal structure of glycine N-methyl-transferase from rat liver
22. Viral and cellular mRNA capping: Past and prospects
23. SEAVIEW and PHYLO_WIN: Two graphic tools for sequence alignment and molecular phylogeny
24. ESPript: Analysis of multiple sequence alignments in PostScript
25. X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes
26. The 1.85 Å structure of vaccinia protein VP39: A bifunctional enzyme that participates in the modification of both mRNA ends
27. Structural basis for sequence-nonspecific recognition of 5′-capped mRNA by a cap-modifying enzyme
28. VP39 - An mRNA cap-specific 2′-O-methyltransferase
29. Protein structure comparison by alignment of distance matrices
30. mRNA cap recognition: Dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains
31. West Nile fever - A reemerging mosquito-borne viral disease in Europe
32. Sequence of the D-asparty/L-isoaspartyl protein methyltransferase from human erythrocytes. Common sequence motifs for protein, DNA, RNA and small molecule S-adenosylmethionine-dependent methyltransferases
33. Dengue and dengue hemorrhagic fever in Latin America and the Caribbean
34. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses
35. Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and γ2 protein of reovirus
36. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
37. PROCHECK: A program to check the stereochemical quality of a protein structure
38. Structure and mechanism of yeast RNA triphosphatase: An essential component of the mRNA capping apparatus
39. Isolation of two strains of West Nile virus during an outbreak in southern Russia, 1999
40. Structure-guided analysis reveals nine sequence motifs conserved among DNA amino-methyltransferases and suggests a catalytic mechanism for these enzymes
41. 7GpppA
42. Raster3D Version 2.0. A program for photorealistic molecular graphics
43. Protein folding and association: Insights from the interfacial and thermodynamics properties of hydrocarbons
44. A structural basis for the preferential binding of hemimethylated DNA by Hha1 DNA methyltransferase
45. Processing of X-ray diffraction data collected in oscillation mode
46. Identification of four conserved motifs among the RNA-dependent polymerase encoding elements
47. Predictive motifs derived from cytosine methyltransferases
48. Structure of the reovirus core at 3.6 Å resolution
49. Dengue and dengue haemorrhagic fever
50. Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M
51. Structure, mechanism and evolution of the mRNA capping apparatus
52. Three-dimensional structure of the complex of guanylate kinase from yeast with its substrate GMP
53. Clustal_W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
54. Three-dimensional structure of adenosylcobinamide kinase/ adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: Evidence for a substrate-induced transferase active site
55. Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 Å resolution
56. The NS 3 nonstructural protein of flaviviruses contains an RNA triphosphatase activity