Characterization of 5-HT1A receptors and their complexes with G-proteins in budded baculovirus particles using fluorescence anisotropy of Bodipy-FL-NAN-190

Neurochemistry International

Volumn 67, Issue 1, 2014, Pages 32-38

  Tõntson, Lauri ^a   Kopanchuk, Sergei ^a,b   Rinken, Ago ^a,b  

^a UNIVERSITY OF TARTU   (Estonia)

^b Competence Centre on Reproductive Medicine and Biology ^*  (Estonia)

Author keywords

5 HT1A receptors; Bodipy FL NAN 190; Budded baculovirus particles; Fluorescence anisotropy; Manganese

Indexed keywords

4,4-DIFLUORO-4-BORA-3A,4A-DIAZA-S-INDACENE; BORON DERIVATIVE; FLUORESCENT DYE; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN BINDING; SEROTONIN 1A RECEPTOR;

BACULOVIRIDAE; CHEMISTRY; FLUORESCENCE POLARIZATION; METABOLISM;

BACULOVIRIDAE; BORON COMPOUNDS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; GTP-BINDING PROTEINS; PROTEIN BINDING; RECEPTOR, SEROTONIN, 5-HT1A;

EID: 84894475279     PISSN: 01970186     EISSN: 18729754     Source Type: Journal    
DOI: 10.1016/j.neuint.2014.01.012     Document Type: Article

References (24)

1. D. Alonso, H. Vazquez-Villa, A.M. Gamo, M.F. Martinez-Esperon, M. Tortosa, A. Viso, R.F. de la Pradilla, E. Junquera, E. Aicart, M. Martin-Fontecha, B. Benhamu, M.L. Lopez-Rodriguez, and S. Ortega-Gutierrez Development of fluorescent ligands for the human 5-HT1A receptor ACS Med. Chem. Lett. 1 2010 249 253
2. L. Bardin, J.P. Tarayre, N. Malfetes, W. Koek, and F.C. Colpaert Profound, non-opioid analgesia produced by the high-efficacy 5-HT(1A) agonist F 13640 in the formalin model of tonic nociceptive pain Pharmacology 67 2003 182 194 (Pubitemid 36302469)
3. H.M. Clawges, K.M. Depree, E.M. Parker, and S.G. Graber Human 5-HT1 receptor subtypes exhibit distinct G protein coupling behaviors in membranes from Sf9 cells Biochemistry 36 1997 12930 12938 (Pubitemid 27465405)
4. 2+: a crystallographic titration experiment Biochemistry 37 1998 14376 14385 (Pubitemid 28489043)
5. S.L. Garland Are GPCRs still a source of new targets? J. Biomol. Screen. 18 2013 947 966
6. J. Nasman, J.P. Kukkonen, S. Ammoun, and K.E.O. Akerman Role of G-protein availability in differential signaling by alpha 2-adrenoceptors Biochem. Pharmacol. 62 2001 913 922 (Pubitemid 32816977)
7. 35S] GTPγS binding study Eur. J. Pharmacol. 355 1998 245 256 (Pubitemid 28404707)
8. 3H]-WAY100,635 Br. J. Pharmacol. 132 2001 518 524 (Pubitemid 32142366)
9. B. Olivier, T. Pattij, S.J. Wood, R. Oosting, Z. Sarnyai, and M. Toth The 5-HT(1A) receptor knockout mouse and anxiety Behav. Pharmacol. 12 2001 439 450 (Pubitemid 33152267)
10. 2+ Neurosci. Lett. 457 2009 32 35
11. 3H]WAY100635 to rat hippocampal membranes Neurochem. Res. 31 2006 1135 1140 (Pubitemid 44379674)
12. 1A receptors by inhibiting guanosine nucleotide binding to receptor-coupled G-proteins Neurotoxicology 32 2011 25 30
13. A. Rinken Formation of the functional complexes of m2 muscarinic acetylcholine receptors with GTP-binding regulatory proteins in solution J. Biochem. (Tokyo) 120 1996 193 200
14. 2 dopamine receptor-G protein coupling. Cross-regulation of agonist and guanosine nucleotide binding site Neurosci. Lett. 302 2001 5 8 (Pubitemid 32244183)
15. L. Rydelek-Fitzgerald, M. Teitler, P.W. Fletcher, A.M. Ismaiel, and R.A. Glennon NAN-190: agonist and antagonist interactions with brain 5-HT1A receptors Brain Res. 532 1990 191 196 (Pubitemid 20384657)
16. K. Shiozaki, and T. Haga Effects of magnesium ion on the interaction of atrial muscarinic acetylcholine receptors and GTP-binding regulatory proteins Biochemistry 31 1992 10634 10642
17. 2A receptors: functional receptor-binding and in vivo electrophysiological studies Psychopharmacology 190 2007 373 382 (Pubitemid 46155646)
18. R.B. Thompson, I. Gryczynski, and J. Malicka Fluorescence polarization standards for high-throughput screening and imaging Biotechniques 32 2002 34 42 (Pubitemid 34052746)
19. i-proteins by Bodipy-FL-GTPγS fluorescence anisotropy Arch. Biochem. Biophys. 524 2012 93 98
20. L. Tõntson, S. Kopanchuk, and A. Rinken Biarsenical ligands bind to endogenous G-protein alpha-subunits and enable allosteric sensing of nucleotide binding BMC Biochem. 14 2013 37
21. 2 muscarinic receptor activation of different G protein subtypes Neurochem. Int. 44 2004 119 124 (Pubitemid 37101388)
22. S. Veiksina, S. Kopanchuk, and A. Rinken Fluorescence anisotropy assay for pharmacological characterization of ligand binding dynamics to melanocortin 4 receptors Anal. Biochem. 402 2010 32 39
23. S. Veiksina, S. Kopanchuk, and A. Rinken Budded baculoviruses as a tool for a homogeneous fluorescence anisotropy-based assay of ligand binding to G protein-coupled receptors: the case of melanocortin 4 receptors Biochim. Biophys. Acta 1838 2014 372 381
24. X.J. Yao, R.G. Velez, M.R. Whorton, S.G. Rasmussen, B.T. DeVree, X. Deupi, R.K. Sunahara, and B. Kobilka The effect of ligand efficacy on the formation and stability of a GPCR-G protein complex Proc. Natl. Acad. Sci. USA 106 2009 9501 9506