Characterization of heterotrimeric nucleotide-depleted Gα i-proteins by Bodipy-FL-GTPγS fluorescence anisotropy

Archives of Biochemistry and Biophysics

Volumn 524, Issue 2, 2012, Pages 93-98

  Tõntson, Lauri ^a   Babina, Anna ^a   Võsumaa, Taavi ^a   Kopanchuk, Sergei ^a,b   Rinken, Ago ^a,b  

^a UNIVERSITY OF TARTU   (Estonia)

^b Competence Centre on Reproductive Medicine and Biology ^*  (Estonia)

Author keywords

Bodipy FL GTP S; Fluorescence anisotropy; G proteins; Manganese; Mastoparan; Nucleotide binding

Indexed keywords

BORON; G PROTEIN ALPHA I1 SUBUNIT; G PROTEIN ALPHA I2 SUBUNIT; G PROTEIN ALPHA I3 SUBUNIT; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANOSINE DERIVATIVE; GUANOSINE DIPHOSPHATE; MAGNESIUM ION; MANGANESE; NUCLEOTIDE; NUCLEOTIDE BINDING PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANISOTROPY; ARTICLE; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN TISSUE; MONITORING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN PURIFICATION; ROOM TEMPERATURE;

ANIMALS; CELL LINE; FLUORESCENCE POLARIZATION; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HUMANS; MAGNESIUM; MANGANESE; PEPTIDES; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE, QUATERNARY; PROTEIN SUBUNITS; WASP VENOMS;

EID: 84863614158     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2012.05.017     Document Type: Article

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