Biarsenical ligands bind to endogenous G-protein α-subunits and enable allosteric sensing of nucleotide binding

BMC Biochemistry

Volumn 14, Issue 1, 2013, Pages

  Tõntson, Lauri ^a   Kopanchuk, Sergei ^a,b   Rinken, Ago ^a,b  

^a UNIVERSITY OF TARTU   (Estonia)

^b Competence Centre on Reproductive Medicine and Biology ^*  (Estonia)

Author keywords

F2FlAsH; Fluorescence anisotropy; G proteins; Nucleotide binding; Tetracysteine

Indexed keywords

4',5' (1,2,3 DITHIOARSOLAN 2 YL) 2',7' DIFLUOROFLUORESCEIN; CYSTEINE; FLUORESCEIN DERIVATIVE; G PROTEIN ALPHA SUBUNIT ALPHA OLF; G PROTEIN ALPHA SUBUNIT ALPHA Q; G PROTEIN ALPHA SUBUNIT ALPHA S LONG; G PROTEIN ALPHA SUBUNIT ALPHA S SHORT; G PROTEIN ALPHA SUBUNIT ALPHA13; G PROTEIN ALPHA SUBUNIT ALPHAI1; G PROTEIN ALPHA SUBUNIT ALPHAI2; G PROTEIN ALPHA SUBUNIT ALPHAI3; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE PHOSPHATE; GUANYLYLIMIDODIPHOSPHATE; UNCLASSIFIED DRUG;

ALLOSTERISM; ANISOTROPY; ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EMISSION ANISOTROPY; LIGAND BINDING; NUCLEOTIDE BINDING SITE; PROTEIN EXPRESSION; PROTEIN INTERACTION; SIGNAL TRANSDUCTION; WILD TYPE;

ALLOSTERIC REGULATION; ANIMALS; ARSENICALS; CYSTEINE; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; GTP-BINDING PROTEIN ALPHA SUBUNITS; GUANOSINE MONOPHOSPHATE; KINETICS; LIGANDS; NUCLEOTIDES; PROTEIN BINDING; RECOMBINANT PROTEINS; SF9 CELLS; SPODOPTERA;

EID: 84890258726     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-14-37     Document Type: Article

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