Out with the old and in with the new: Rapid specimen preparation procedures for electron microscopy of sectioned biological material

Protoplasma

Volumn 251, Issue 2, 2014, Pages 429-448

  McDonald, Kent L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Biological specimen preparation; Electron microscopy; FS; High pressure freezing; LR White; On section immunolabeling; Resin infiltration; Resin polymerization

Indexed keywords

ANIMAL; CRYOPRESERVATION; DEVICES; ELECTRON MICROSCOPY; FREEZE DRYING; IMMUNOHISTOCHEMISTRY; MICROTOMY; MOUSE; PROCEDURES; TISSUE FIXATION;

ANIMALS; CRYOPRESERVATION; FREEZE SUBSTITUTION; IMMUNOHISTOCHEMISTRY; MICE; MICROSCOPY, ELECTRON; MICROTOMY; TISSUE FIXATION;

EID: 84894286449     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-013-0575-y     Document Type: Article

References (129)

1. Al-Amoudi A et al (2004) Cryo-electron microscopy of vitreous sections. EMBO J 23: 3583-3588.
2. Altman LG, Schneider BG, Papermaster DS (1984) Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy. J Histochem Cytochem 32: 1217-1223.
3. Altmann R (1890) Die Elementarorganismen und ihre Beziehungen zu den Zellen. Veit, Leipzig.
4. Armbruster BL, Carlemalm E, Chiovetti R, Garavito RM, Hobot JA, Kellenberger E, Villiger W (1982) Specimen preparation for electron microscopy using low temperature embedding resins. J Microsc (Oxford) 126: 77-85.
5. Bain JM, Gove DW (1971) Rapid preparation of plant tissues for electron microscopy. J Microsc 93: 159.
6. Bajer A, Mole-Bajer J (1972) Spindle dynamics and chromosome movements. Academic, New York.
7. Boekema EJ, Folea M, Kouril R (2009) Single particle electron microscopy. Photosynth Res 102: 189-196.
8. Bozzola JJ, Russell LD (1999) Electron microscopy: principles and techniques for biologists, 2nd edn. Jones and Bartlett, Salisbury.
9. Bretschneider LD, Elders PF (1952) Elektronenmikroskopishe Zellanalyse nach der Gefriertrocknungmethode. Proc Kon Ned Akad v Wet 55: 675-688.
10. Brightman MW, Reese TS (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40: 648-677.
11. Bullivant S (1960) The staining of thin sections of mouse pancreas prepared by the Fernández-Moran helium II freeze-substitution method. J Biophys Biochem Cytol 8: 639-647.
12. Buser C, Walther P (2008) Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 °C. J Microsc 230: 268-277.
13. Craig S, Gilkey JC, Staehelin LA (1987) Improved specimen support cups and auxiliary devices for the Balzers high pressure freezing apparatus. J Microsc 48: 103-106.
14. Dubochet J (2007) The physics of rapid cooling and its implications for cryoimmobilization of cells. Meth Cell Biol 79: 7-21.
15. Echlin P (1992) Low temperature microscopy and analysis. Plenum, New York.
16. Erickson PA, Anderson DH, Fisher SK (1987) Use of uranyl acetate en bloc to improve tissue preservation and labeling for post-embedding immunoelectron microscopy. J Electron Microsc Technol 5: 303-314.
17. Farquhar MG, Palade GE (1965) Cell junctions in amphibian skin. J Cell Biol 26: 263-291.
18. Feder N, Sidman RL (1958) Methods and principles of fixation by freeze-substitution. J Biophys Biochem Cytol 4: 593-600.
19. Fernandez Moran H (1952) Application of the ultrathin freezing-sectioning technique to the study of cell structures with the electron microscope. Ark f Fysik 4: 471-483.
20. Fernandez-Moran H (1959) Cryofixation and supplementary low temperature preparation techniques applied to the study of tissue ultrastructure. J Appl Phys 30: 2038.
21. Fernandez-Moran H (1960) Low temperature preparation techniques for electron microscopy of biological specimens based on rapid freezing with liquid helium II. Ann N Y Acad Sci 8(5): 689-713.
22. Frank J (2006) Three-dimensional electron microscopy of macromolecular assemblies: visualization of biological molecules in their native state. Oxford University Press, Oxford.
23. Franzini-Armstrong C, Porter KR (1964) Sarcolemmal invaginations constituting the T system in fish muscle fibers. J Cell Biol 22: 675-696.
24. Gan L, Jensen GJ (2012) Electron tomography of cells. Q Rev Biophys 45: 27-56.
25. Giberson RT, Demaree RS (2001) Microwave techniques and protocols. Humana, Totowa.
26. Giberson RT, Smith RL, Demaree RS (1995) Three hour microwave tissue processing for transmission electron microscopy: from unfixed tissues to sections. Scanning 17(suppl 5): 26-27.
27. Gilkey JC, Staehelin LA (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J Electron Microsc Technol 3: 177-210.
28. Glauert AM (1975) Fixation, dehydration and embedding of biological specimens. In: Glauert AM (ed) Practical methods in electron microscopy. North-Holland, Amsterdam.
29. Glauert AM, Rogers GE, Glauert RH (1956) A new embedding medium for electron microscopy. Nature 178: 803.
30. Han H-M, Huebinger J, Grabenbauer M (2012) Self-pressurized rapid freezing (SPRF) as a simple fixation method for cryo-electron microscopy of vitreous sections. J Struct Biol 178: 84-87.
31. Hawes P, Netherton CL, Mueller M, Wileman T, Monaghan P (2007) Rapid freeze-substitution preserves membranes in high-pressure frozen tissue culture cells. J Microsc 226: 182-189.
32. Hayat MA (1981) Fixation for electron microscopy. Academic, San Diego.
33. Hayat MA (2000) Principles and techniques of electron microscopy, 4th edn. CRC, Boca Raton.
34. Hayat MA, Giaquinta R (1970) Rapid fixation and embedding for electron microscopy. Tissue Cell 2: 191-195.
35. Hess MW (2007) Cryopreparation methodology for plant cell biology. Meth Cell Biol 79: 57-100.
36. Hippe-Sanwald S (1993) Impact of freeze substitution on biological electron microscopy. Microsc Res Tech 24: 400-422.
37. Hobot JA, Newman GR (1991) Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy. In: Roomans G, Edelmann L (eds) Scanning microscopy supplement 5. Scanning Microscopy International, AMF O'Hare, pp S27-S41.
38. Humbel BM, Mueller M (1986) Freeze substitution and low temperature embedding, Science of Biological Specimen Preparation 1985. SEM, AMF O'Hare.
39. Humbel BM, Schwarz H (1989) Freeze-substitution for immunochemistry. In: Verkleij AJ, Leunissen JLM (eds) Immuno-gold labeling in cell biology. CRC, Boca Raton.
40. Humbel BM, Marti T, Mueller M (1983) Improved structural preservation by combining freeze substitution and low temperature embedding. Beitr Elektronenmikrosk Direktabb Oberfl 16: 585-594.
41. Hurbain I, Saches M (2011) The future is cold: cryo-preparation methods for transmission electron microscopy of cells. Biol Cell 103: 405-420.
42. Kaech A (2009) BAL-TEC HPM 010 high-pressure freezing machine. In: Cavalier A, Spehner D, Humbel BM (eds) Handbook of Cryo-Preparation Methods for Electron Microscopy, CRC Press, Boca Raton, FL, pp 101-128.
43. Karnovsky MJ (1967) The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol 35: 213-236.
44. Kellenberger E (1987) The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation. In: Steinbrecht RA, Zierold K (eds) Cryotechniques in biological electron microscopy. Springer, Berlin.
45. Kellenberger E, Carlemalm E, Villiger W, Roth J, Garavito RM (1980) Low denaturation embedding for electron microscopy of thin sections. Chemische Werke Löwi, Waldkraiburg.
46. Kellenberger E, Ryter A, Sechaud J (1958) Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol 4: 671-678.
47. Kukulski W, Schorb M, Welsch S, Picco A, Kaksonen M, Briggs JA (2011) Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. J Cell Biol 192: 111-119.
48. Kukulski W, Schorb M, Welsch S, Picco A, Kaksonen M, Briggs JA (2012) Precise, correlated fluorescence microscopy and electron microscopy of Lowicryl sections using fluorescent fiducial markers. Meth Cell Biol 111: 235-257.
49. Lancelle S, Hepler PK (1989) Immunogold labelling of actin on sections of freeze-substituted plant cells. Protoplasma 150: 72-74.
50. Leunissen JL, Yi H (2009) Self-pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy. J Microsc 235: 25-35.
51. Login GR, Dvorak AM (1994) Methods of microwave fixation for microscopy: a review of research and clinical applications. In: Graumann W, Lojda Z, Pearse AGE, Schiebler TH (eds) Progress in histochemistry and cytochemistry. Fischer, New York, pp 1970-1992.
52. Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9: 409-414.
53. Maaløe O, Birch-Anderson A (1956) On the organization of the 'nuclear material' in Salmonella typhimurium. Symp Soc Gen Microbiol 6: 261-278.
54. MacKenzie AP (1967) The "collapse phenomenon" in the freeze-substitution process. Cryobiology 3: 387.
55. McCurdy DW, Pratt LH (1986) Immunogold electron microscopy of phytochrome in Avena: identification of intracellular sites responsible for phytochrome sequestering and enhanced pelletability. J Cell Biol 103: 2541-2550.
56. McDonald KL (1999) High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Meth Mol Biol 117: 77-97.
57. McDonald KL (2007) Cryopreparation methods for electron microscopy of selected model systems. Meth Cell Biol 79: 23-55.
58. McDonald K (2009) A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. J Microsc 235: 273-281.
59. McDonald KL (2013) Rapid embedding methods into epoxy and LR White resins for morphological and immunological analysis of cryofixed biological specimens. Microsc Microanal 20: 1-12. doi: 10. 1017/S1431927613013846.
60. McDonald KL (1994) Electron microscopy and EM immunocytochemistry. In: Goldstein LSB, Fyrberg E (eds) Drosophila melanogaster: practical uses in cell and molecular biology. Meth Cell Biol 44: 411-444.
61. McDonald K, Müller-Reichert T (2002) Cryomethods for thin section electron microscopy. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular and cell biology parts B and C. Meth Enzymol 351: 96-123.
62. McDonald KL, Webb RI (2011) Freeze substitution in 3 hours or less. J Microsc 243: 227-233.
63. McDonald KL, Morphew M, Verkade P, Müller-Reichert T (2007) Recent advances in high pressure freezing: equipment and specimen loading methods. Meth Mol Biol 369: 143-173.
64. McDonald KL, Schwarz H, Mueller-Reichert T, Webb R, Buser C, Morphew M (2010) Tips and tricks for high pressure freezing. Meth Cell Biol 96: 671-693.
65. Mersey B, McCully ME (1978) Monitoring the course of fixation in plant cells. J Microsc 116: 49-76.
66. Moberg C (2012) Entering an unseen world. Rockefeller University Press, New York.
67. Mobius W (2009) Cryopreparation of biological specimens for immunoelectron microscopy. Ann Anat 191: 231-247.
68. Monaghan P, Robertson D (1990) Freeze substitution without aldehyde or osmium fixatives: ultrastructure and implications for immnocytochemistry. J Microsc 158: 355-363.
69. Monaghan P, Perusinghe N, Müller M (1998) High-pressure freezing for immunocytochemistry. J Microsc 192: 248-258.
70. Moor H (1987) Theory and practice of high pressure freezing. In: Steinbrecht R, Zierold K (eds) Cryotechniques in biological electron microscopy. Springer, Berlin, pp 175-191.
71. Moor H, Hoechli M (1970) The influence of high-pressure freezing on living cells. In: Favard P (ed) Proceedings of the 7th International Congress on Electron Microscopy, Grenoble. Societe Francaise de Microscopie Electronique, Paris.
72. Moor H, Muhlethaler K, Waldner H, Frey-Wyssling A (1961) A new freezing-ultramicrotome. J Biophys Biochem Cytol 10: 1-13.
73. Mueller M, Moor H (1984) Cryofixation of thick specimens by high pressure freezing. In: Revel J-P, Barnard T, Haggis GH (eds) The science of biological specimen preparation. SEM, AMF O'Hare, pp. 131-138.
74. Mueller-Reichert T, O'Toole E, Hohenberg H, McDonald KL (2003) Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J Microscopy 212: 71-80.
75. Muhlethaler K (1973) History of freeze-etching. In: Benedetti L, Favard P (eds) Freeze-etching techniques and applications. Societe Francaise de Microscopie Electronique, Paris, pp 1-10.
76. Müller HO (1942) Die Ausmessung der Tiefe übermikroskopischer Objekte. Kolloid-Z 99: 6-28.
77. Newman GR, Hobot JA (1993) Resin microscopy and on-section immunocytochemistry. Springer, Berlin.
78. Newman GR, Hobot JA (1999) Resins for combined light and electron microscopy: a half century of development. Histochem J 31: 495-505.
79. Newman JB, Borysko E, Swerdlow M (1949) New sectioning techniques for light and electron microscopy. Science 110: 66-68.
80. Newman GR, Jasani B, Williams ED (1982) The preservation of ultrastructure and antigenicity. J Microsc 127: RP5-RP6.
81. Newman GR, Jasani B, Williams ED (1983) A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochem J 15: 543-555.
82. Nicolas M-T, Bassot J-M (1993) Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry. Microsc Res Tech 24: 474-487.
83. Nixon SJ, Webb RI, Floetenmeyer M, Schieber N, Lo HP, Parton RG (2009) A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos. Traffic 10: 131-136.
84. O'Toole ET (2002) Electron tomography of yeast cells. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular and cell biology parts B and C. Meth Enzymol 351: 81-95.
85. Oprins A, Geuze HJ, Slot JW (1994) Cryosubstitution dehydration of aldehyde-fixed tissue: a favorable approach to quantitative immunocytochemistry. J Histochem Cytochem 42: 497-503.
86. Palade GE (1952) A study of fixation for electron microscopy. J Exp Med 95: 285-298.
87. Pease DC (1973) Substitution techniques. In: Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer, Berlin, pp 35-66.
88. Pease DC, Porter KR (1981) Electron microscopy and ultramicrotomy. J Cell Biol 91: 287s-292s.
89. Porta D, Lopez-Iglesias C (1998) A comparison of cryo- versus chemical fixation in the soil green algae Jaagiella. Tissue Cell 30: 368-376.
90. Rasmussen N (1997) Picture control. Stanford University Press, Stanford.
91. Rebhun LI (1961) Applications of freeze-substitution to electron microscope studies of invertebrate oocytes. J Biophys Biochem Cytol 9: 785-798.
92. Rebhun LI (1972) Freeze-substitution and freeze-drying. In: Hayat MA (ed) Principles and techniques of electron microscopy, vol II. van Nostrand Reinhold, New York, pp 3-49.
93. Reymond OL, Pickett-Heaps JD (1983) A routine flat embedding method for electron microscopy of microorganisms allowing selection and precisely orientated sectioning of single cells by light microscopy. J Microsc 130: 79-84.
94. Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 16: 208-212.
95. Riehle U (1968) Über die Vitrifizierung verdünnter wässeriger Lösungen. Techn Wiss Diss Eidgen Tech Hochschule Zürich.
96. Riehle U, Hoechli M (1973) The theory and technique of high pressure freezing. In: Benedetti L, Favard P (eds) Freeze-etching techniques and applications. Societe Francaise de Microscopie Electronique, Paris, pp 32-61.
97. Robards AW, Sleytr UB (1985) Low temperature methods in biological electron microscopy. In: Glauert AM (ed) Practical methods in electron microscopy, vol 10. Elsevier, Amsterdam.
98. Robbins E, Jentzsch G (1967) Rapid embedding of cell culture monolayers and suspensions for electron microscopy. J Histochem Cytochem 15: 181-182.
99. Sabatini DD, Bensch K, Barrnett RJ (1963) Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymic activity by aldehyde fixation. J Cell Biol 17: 19-58.
100. Sawaguchi A, Ide S, Suganuma T (2005) Application of 10-μm thin stainless foil to a new assembly of the specimen carrier in high-pressure freezing. J Electron Microsc (Tokyo) 54: 143146.
101. Shepherd B, Clark C (1976) Structure of the anterior alimentary tract of the passively feeding nematode Pratylenchus penetrans (Nematoda: Pratylenchidae). Nematologica 31: 321-334.
102. Shimoni E, Mueller M (1998) On optimizing high-pressure freezing: from heat transfer theory to a new microbiopsy device. J Microsc 192: 236-247.
103. Shiurba R (2001) Freeze-substitution: origins and applications. Int Rev Cytol 206: 45-96.
104. Silva MT, Carvalho Guerra F, Magalhaes MM (1968) The fixative action of uranyl acetate in electron microscopy. Experientia 24: 1074.
105. Silva MT, Santos Mota JM, Melo JVC, Carvalho Guerra F (1971) Uranyl salts as fixatives for electron microscopy. Study of the membrane ultrastructure and phospholipid loss in bacilli. Biochim Biophys Acta 233: 513-520.
106. Simpson WL (1941) An experimental analysis of the Altmann technique of freezing-drying. Anat Rec 80: 173-189.
107. Sosinsky GE et al (2008) The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications. J Struct Biol 161: 359-371.
108. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31-43.
109. Steere RL (1957) Electron microscopy of structural detail in frozen biological specimens. J Biophy Biochem Cytol 3: 45-60.
110. Steinbrecht RA (1982) Experiments on freezing damage with freeze substitution using moth antennae as test objects. J Microsc 125: 187-192.
111. Steinbrecht RA, Mueller M, Steinbrecht RA, Mueller M (1987) Freeze substitution and freeze drying. In: Steinbrecht R, Zierold K (eds) Cryotechniques in biological electron microscopy. Springer, Berlin, pp 150-172.
112. Studer D, Michel M, Müller M (1993) High pressure freezing comes of age. Scanning Microsc Suppl 3: 253-269.
113. Studer D, Graber W, Al-Amoudi A, Eggli P (2001) A new approach for cryofixation by high-pressure freezing. J Microsc 203: 285-294.
114. Terzakis JA (1968) Uranyl acetate, a stain and a fixative. J Ultrastruct Res 22: 168-184.
115. Tormey JMD (1964) Differences in membrane configuration between osmium tetroxide-fixed and glutaraldehyde-fixed ciliary epithelium. J Cell Biol 23: 658-664.
116. Valentino KL, Crumrine DA, Reichardt LF (1985) Lowicryl K4M embedding of brain tissue for immunogold electron microscopy. J Histochem Cytochem 33: 969-973.
117. van Genderen IL, vanMeer G, Slot JW, Geuze HJ, Voorhout WF (1991) Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution. J Cell Biol 115: 1009-1019.
118. van Harreveld A, Crowell J (1964) Electron microscopy after rapid freezing on a metal surface and substitution fixation. Anat Rec 149: 381-386.
119. van Lookeren CM, van der Oestreicher AB, Krift TP, Gispen WH, Verkleij AJ (1991) Freeze-substitution and Lowicryl HM20 embedding of fixed rat brain: suitability for immunogold ultrastructural localization of neural antigens. J Histochem Cytochem 39: 1267-1279.
120. Vanhecke D, Studer D (2009) High pressure freezing Leica EM PACT. In: Cavalier A, Spehner D, Humbel BM (eds) Handbook of cryo-preparation methods for electron microscopy. CRC, Boca Raton, pp 129-156.
121. Vanhecke D, Graber W, Studer D (2008) Close-to-native ultrastructural preservation by high pressure freezing. Meth Cell Biol 88: 151-164.
122. Verkade P (2008) Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high pressure freezing. J Microsc 230: 317-328.
123. Voorhout WF, Veenendaal T, Haagsman HP, van Verkleij AJ, Golde LM, Geuze HJ (1991) Surfactant protein A is localized at the corners of the pulmonary tubular myelin lattice. J Histochem Cytochem 39: 1331-1336.
124. Walther P, Ziegler A (2002) Freeze-substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J Microsc 208: 3-10.
125. Weibull C, Villiger W, Carlemalm E (1984) Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy. J Microsc 134: 213-216.
126. Williams RC, Wyckoff RWG (1944) The thickness of electron microscope objects. J Appl Phys 15: 713.
127. Wyckoff RWG (1946) Frozen-dried preparations for the electron microscope. Science 104: 36-37.
128. Yakovlev S, Downing KH (2011) Freezing in sealed capillaries for preparation of frozen hydrated sections. J Microsc 244: 235-247.
129. Zalokar M (1966) A simple freeze-substitution method for electron microscopy. J Utrastruct Res 15: 469-479.