Rapid embedding methods into epoxy and LR white resins for morphological and immunological analysis of cryofixed biological specimens

Microscopy and Microanalysis

Volumn 20, Issue 1, 2014, Pages 152-163

  McDonald, Kent L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Biological specimen preparation; Correlative light and electron microscopy; Electron microscopy; Freeze substitution; High pressure freezing; LR White; On section immunolabeling; Rapid embedding methods; Resin infiltration; Resin polymerization

Indexed keywords

EPOXY RESIN;

ANIMAL; ARTICLE; BACTERIUM; FREEZE DRYING; HISTOLOGY; IMMUNOHISTOCHEMISTRY; METHODOLOGY; PLANT LEAF;

ANIMALS; BACTERIA; EPOXY RESINS; FREEZE SUBSTITUTION; IMMUNOHISTOCHEMISTRY; PLANT LEAVES; TISSUE EMBEDDING;

EID: 84896490501     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927613013846     Document Type: Article

References (49)

1. Bain, J.M. & Gove, D.W. (1971). Rapid preparation of plant tissues for electron microscopy. J Microsc 93, 159-162.
2. Boutte, Y., Jonsson, K., McFarlane, H.E., Johnson, E., Gendre, D., Swarup, R., Friml, J., Samuels, L., Robert, S. & Bhalerao, R.P. (2013). ECHIDNA-mediated post-Golgi trafficking of auxin carriers for differential cell elongation. Proc Natl Acad Sci (USA) 110, 16259-16264.
3. Bozzola, J.J. & Russell, L.D. (1999). Electron Microscopy: Principles and Techniques for Biologists, 2nd ed. Salisbury, MA: Jones and Bartlett.
4. Coulter, H.D. (1967). Rapid and improved methods for embedding biological tissues in Epon 812 and Araldite 502. J Ultrastruct Res 20, 346-355.
5. Craig, S., Gilkey, J.C. & Staehelin, L.A. (1987). Improved specimen support cups and auxiliary devices for the Balzers high pressure freezing apparatus. J Microsc 48, 103-106.
6. Dubochet, J. (2007). The physics of rapid cooling and its implications for cryoimmobilization of cells. Meth Cell Biol 79, 7-21.
7. Ellisman, M.H., Deerinck, T.J., Shu, X. & Sosinsky, G.E. (2012). Picking faces out of a crowd: Genetic labels for identification of proteins in correlated light and electron microscopy imaging. Meth Cell Biol 111, 139-155.
8. Falbel, T.G., Koch, L.M., Nadeau, J.A., Segui-Simarro, J.M., Sack, F.D. & Bednarek, S.Y. (2003). SCD1 is required for cell cytokinesis and polarized cell expansion in Arabidopsis thaliana. Development 130, 4011-4024.
9. Giberson, R.T. & Demaree, R.S., Jr. (Eds) (2001). Microwave Techniques and Protocols. Totowa, NJ: Humana Press.
10. Giberson, R.T., Smith, R.L. & Demaree, R.S. (1995). Three hour microwave tissue processing for transmission electron microscopy: From unfixed tissues to sections. Scanning 17(Suppl 5), 26-27.
11. Glauert, A.M. (1975). Fixation, dehydration and embedding of biological specimens. In Practical Methods in Electron Microscopy, vol. 3, Part 1, Glauert, A.M. (Ed). Amsterdam:North-Holland.
12. Hawes, P., Netherton, C.L., Mueller, M., Wileman, T. & Monaghan, P. (2007). Rapid freeze-substitution preserves membranes in high-pressure frozen tissue culture cells. J Microsc 226, 182-189.
13. Hayat, M.A. (1986). Glutaraldehyde: Role in electron microscopy. Micron Microsc Acta 17, 115-135.
14. Hayat, M.A. (2000). Principles and Techniques of Electron Microscopy, 4th ed. Boca Raton, FL: CRC Press.
15. Hayat, M.A. & Giaquinta, R. (1970). Rapid fixation and embedding for electron microscopy. Tissue and Cell 2, 191-195.
16. Hess, M.W. (2007). Cryopreparation methodology for plant cell biology. Meth Cell Biol 79, 57-100.
17. Hurbain, I. & Sachse, M. (2011). The future is cold: Cryopreparation methods for transmission electron microscopy of cells. Biol Cell 103, 405-420.
18. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A. (2011). Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. J Cell Biol 192, 111-119.
19. Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M. & Briggs, J.A.G. (2012). Precise, correlated fluorescence microscopy and electron microscopy of Lowicryl sections using fluorescent fiducial markers. Meth Cell Biol 111, 235-257.
20. Leunissen, J.L. & Yi, H. (2009). Self-pressurized rapid freezing (SPRF): A novel cryofixation method for specimen preparation in electron microscopy. J Microsc 235, 25-35.
21. Marchese, S.P. & Johnson, S.P.S. (1982). A simple method for the progressive infiltration of resin into a dehydrated biological sample. Proc Roy Microsc Soc 17, 311.
22. McCully, M.E. & Canny, M.J. (1985). The stabilization of labile configurations of plant cytoplasm by freeze-substitution. J Microsc 139, 27-33.
23. McDonald, K. (2007). Cryopreparation methods for electron microscopy of selected model systems.Meth Cell Biol 79, 23-56.
24. McDonald, K., Schwarz, H., Mueller-Reichert, T., Webb, R., Buser, C. & Morphew, M. (2010). "Tips and tricks" for high pressure freezing of model systems. Meth Cell Biol 96, 671-694.
25. McDonald, K.L. (1999). High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. In ElectronMicroscopyMethods and Protocols, Hajibagheri, M.A.N. (Ed), pp. 77-97. Totowa, NJ: Humana Press.
26. McDonald, K.L. & Webb, R.I. (2011). Freeze substitution in 3 hours or less. J Microsc 243, 227-233.
27. Millonig, G. (1976). Laboratory Manual of Biological Electron Microscopy. Vercelli, Italy: Saviolo.
28. MObius, W. (2009). Cryopreparation of biological specimens for immunoelectron microscopy. Ann Anat 191, 231-247.
29. Mueller-Reichert, T. & Verkade, P. (Eds) (2012). Correlative Light Electron Microscopy. San Diego: Academic Press.
30. MUller-Reichert, T., O'Toole, E.T., Hohenberg, H. & Mc-Donald, K.L. (2003). Cryoimmobilization and threedimensional visualization of C. elegans ultrastructure.J Microsc 212, 71-80.
31. Newman, G.R. & Hobot, J.A. (1993). Resin Microscopy and On-Section Immnocytochemistry. Berlin: Springer-Verlag.
32. Newman, G.R. & Hobot, J.A. (1999). Resins for combined light and electron microscopy: A half century of development. Histochem J 31, 495-505.
33. Nicolas, M-T. & Bassot, J-M. (1993). Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry. Microsc Res Tech 24, 474-487.
34. Nixon, S.J., Webb, R.I., Floetenmeyer, M., Schieber, N., Lo, H.P. & Parton, R.G. (2009). A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos. Traffic 10, 131-136.
35. Ondzighi, C.A., Christopher, D.A., Cho, E.J., Chang, S-C. & Staehelin, L.A. (2008). Arabidopsis protein disulfide isomerase-5 inhibits cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds. Plant Cell 20, 2205-2220.
36. Otegui, M.S., Herdera, R., Schulzec, J., Jungc, R. & Staehelin, L.A. (2006). The proteolytic processing of seed storage in Arabidopsis embryo cells starts in the multivesicular bodies. Plant Cell 18, 2567-2581.
37. Parton, R.G. (1995). Rapid processing of filter-grown cells for Epon embedding. J Histochem Cytochem 43, 731-733.
38. Porta, D. & Lopez-Iglesias, C. (1998). A comparison of cryoversus chemical fixation in the soil green algae Jaagiella. Tissue Cell 30, 368-376.
39. Reymond, O.L. & Pickett-Heaps, J.D. (1983). A routine flat embedding method for electron microscopy of microorganisms allowing selection and precisely orientated sectioning of single cells by light microscopy. J Microsc 130, 79-84.
40. Reynolds, E.S. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17, 208-212.
41. Robbins, E. & Jentzsch, G. (1967). Rapid embedding of cell culture monolayers and suspensions for electron microscopy. J Histochem Cytochem 15, 181-182.
42. Roudier, F., Fernandez, A.G., Fujita, M., Himmelspach, R., Borner, G.H.H., Schindelman, G., Song, S., Baskin, T.I., Dupree, P., Wasteneys, G.O. & Benfey, P. N. (2005). COBRA, an Arabidopsis extracellular glycosyl-phosphatidyl inositolanchored protein, specifically controls highly anisotropic expansion through its involvement in cellulose microfibril orientation. Plant Cell 17, 1749-1763.
43. Rowden, G. & Lewis, M.G. (1974). Experience with a three-hour electron microscopy biopsy service. J Cli Pathol 27, 505-510.
44. Segui-Simarro, J.M., Austin, J.R. II, White, E.A. & Staehelin, L.A. (2004). Electron tomographic analysis of somatic cell plate formation in meristematic cells of arabidopsis preserved by high-pressure freezing. Plant Cell 16, 836-856.
45. Shimoni, E., Rav-Hon, O., Ohad, I., Brumfeld, V. & Reich, Z. (2005). Three-dimensional organization of higher-plant chloroplast thylakoid membranes revealed by electron tomography. Plant Cell 17, 2580-2586.
46. Skepper, J.N. (2000). Immunocytochemical strategies for electron microscopy: Choice or compromise. J Microsc 199, 1-36.
47. Spurr, A.R. (1969). A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastr Res 26, 31-43.
48. Webster, P. (2007). Microwave-Assisted processing and embedding for transmission electron microscopy. In Methods in Molecular Biology: Electron Microscopy-Methods and Protocols, Kuo, J. (Ed), pp. 257-289. Totowa, NJ: Humana Press.
49. Weibull, C., Villiger, W. & Carlemalm, E. (1984). Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy. J Microsc 134, 213-216.