Self-pressurized rapid freezing (SPRF) as a simple fixation method for cryo-electron microscopy of vitreous sections

Journal of Structural Biology

Volumn 178, Issue 2, 2012, Pages 84-87

  Han, Hong Mei ^a   Huebinger, Jan ^a   Grabenbauer, Markus ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

Cryo electron microscopy of vitreous sections (CEMOVIS); High pressure freezing (HPF); Model organisms; Self pressurized rapid freezing (SPRF)

Indexed keywords

COPPER; ETHANE; GLASS; LIQUID NITROGEN;

ANIMAL CELL; APPARATUS; ARTICLE; BACTERIAL CELL; COOLING; CRYO ELECTRON MICROSCOPY OF VITREOUS SECTIONS; ELECTRON DIFFRACTION; ELECTRON MICROSCOPY; LIQUID; LOW TEMPERATURE PROCEDURES; NONHUMAN; PHASE TRANSITION; PRESSURE; PRIORITY JOURNAL; SELF PRESSURIZED RAPID FREEZING; YEAST CELL;

CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; PRESSURE;

MAMMALIA;

EID: 84860546905     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2012.04.001     Document Type: Article

References (15)

1. Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.M., et al. Cryo-electron microscopy of vitreous sections. EMBO J. 2004, 23:3583-3588.
2. Al-Amoudi A., Diez D.C., Betts M.J., Frangakis A.S. The molecular architecture of cadherins in native epidermal desmosomes. Nature 2007, 450:832-837.
3. Bouchet-Marquis C., Zuber B., Glynn A.M., Eltsov M., Grabenbauer M., et al. Visualization of cell microtubules in their native state. Biol. Cell. 2007, 99:45-53.
4. Bouchet-Marquis C., Starkuviene V., Grabenbauer M. Golgi apparatus studied in vitreous sections. J. Microsc. 2008, 230:308-316.
5. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., et al. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 1988, 21:129-228.
6. Dubochet J., Zuber B., Eltsov M., Bouchet-Marquis C., Al-Amoudi A., et al. How to " read" a vitreous section. Methods Cell. Biol. 2007, 79:385-406.
7. Hohn K., Sailer M., Wang L., Lorenz M., Schneider M.E., et al. Preparation of cryofixed cells for improved 3D ultrastructure with scanning transmission electron tomography. Histochem. Cell. Biol. 2011, 135:1-9.
8. Hsieh C.E., Leith A., Mannella C.A., Frank J., Marko M. Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 2006, 153:1-13.
9. Kukulski W., Schorb M., Welsch S., Picco A., Kaksonen M., et al. Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. J. Cell. Biol. 2011, 192:111-119.
10. Leunissen J.L., Yi H. Self-pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy. J. Microsc. 2009, 235:25-35.
11. McDowall A.W., Chang J.J., Freeman R., Lepault J., Walter C.A., et al. Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples. J. Microsc. 1983, 131:1-9.
12. Moor, H., Riehle, U., 1968. Snap freezing under high-pressure: a new fixation method for freeze-etching. Proceedings of the 4th Eur. Reg. Conference Electron Microscopy, Rome, 2, pp. 33-34.
13. Pierson J., Ziese U., Sani M., Peters P.J. Exploring vitreous cryo-section-induced compression at the macromolecular level using electron cryo-tomography; 80S yeast ribosomes appear unaffected. J. Struct. Biol. 2011, 173:345-349.
14. Yakovlev S., Downing K.H. Freezing in sealed capillaries for preparation of frozen hydrated sections. J. Microsc. 2011, 244:235-247.
15. Zuber B., Nikonenko I., Klauser P., Muller D., Dubochet J. The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes. Proc. Natl. Acad. Sci. U. S. A. 2005, 102:19192-19197.