Activity assays of mammalian thioredoxin and thioredoxin reductase: Fluorescent disulfide substrates, mechanisms, and use with tissue samples

Analytical Biochemistry

Volumn 449, Issue 1, 2014, Pages 139-146

  Montano, Sergio J ^a   Lu, Jun ^a   Gustafsson, Tomas N ^a   Holmgren, Arne ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Enzymatic activity; Fluorescence assay; Redox; Thioredoxin; Thioredoxin reductase

Indexed keywords

AMINO ACIDS; FLUORESCENCE; INSULIN; MAMMALS; SUBSTRATES; SULFUR COMPOUNDS; TISSUE;

ENZYMATIC ACTIVITIES; FLUORESCENCE ASSAY; REDOX; THIOREDOXIN REDUCTASE; THIOREDOXINS;

TISSUE ENGINEERING;

DI EOSIN GLUTATHIONE DISULFIDE; DISULFIDE; FLUORESCEIN ISOTHIOCYANATE INSULIN; THIOREDOXIN; THIOREDOXIN REDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; AUTOREGULATION; BURKITT LYMPHOMA; CHRONIC MYELOID LEUKEMIA; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; HISTIOCYTIC LYMPHOMA; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; LYMPHOCYTE; LYMPHOMA CELL LINE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTUM YIELD;

ENZYMATIC ACTIVITY; FLUORESCENCE ASSAY; REDOX; THIOREDOXIN; THIOREDOXIN REDUCTASE;

ANIMALS; CELL LINE, TUMOR; ENZYME ASSAYS; EOSINE YELLOWISH-(YS); FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; GLUTATHIONE DISULFIDE; HUMANS; INSULIN; OXIDATION-REDUCTION; THIOREDOXIN-DISULFIDE REDUCTASE; THIOREDOXINS;

EID: 84892924777     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.12.025     Document Type: Article

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