Fluoresceinthiocarbamyl-insulin: A potential analytical tool for the assay of disulfide bond reduction

Journal of Biochemical and Biophysical Methods

Volumn 34, Issue 3, 1997, Pages 213-225

  Heuck, Alejandro P ^a   Wolosiuk, Ricardo A ^a  

^a FUNDACIÓN INSTITUTO LELOIR   (Argentina)

Author keywords

Disulfide bonds; Fluorescein; Fluorometric assay; Insulin; Protein disulfide isomerase; Thioredoxin

Indexed keywords

INSULIN DERIVATIVE; OXIDOREDUCTASE;

AMINO ACID COMPOSITION; ARTICLE; DERIVATIZATION; DISULFIDE BOND; DRUG SYNTHESIS; ENZYME ASSAY; FLUORESCENCE; IN VITRO STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; TECHNIQUE;

ANIMALS; CATTLE; DISULFIDES; DITHIOTHREITOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENCE; INSULIN; OXIDATION-REDUCTION; SODIUM DODECYL SULFATE;

BOVINAE;

EID: 0030866362     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(97)00014-6     Document Type: Article

References (13)

1. Bardwell J.C.A.. Building bridges: disulphide bond formation in the cell. Mol. Microbiol. 1994;14:199-205.
2. Freedman R.B.. Folding helpers and unhelpful folders. Curr. Biol. 1994;4:933-935.
3. Holmgren A.. Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide. J. Biol. Chem. 1979;254:9627-9632.
4. Tsibris J.C.M., Hunt L.T., Ballejo G., Barker W.C., Toney L.J., Spellacy W.N.. Selective inhibition of protein disulfide isomerase by estrogens. J. Biol. Chem. 1989;264:13967-13970.
5. Holmgren A., Ohlsson I., Grankvist M.-L.. Radioimmunological and enzymatic determinations in wild type cells and mutants defective in phage T7 DNA replication. J. Biol. Chem. 1978;253:430-436.
6. Tietze F., Mortimore G.E., Lomax N.R.. Preparation and properties of fluorescent insulin derivatives. Biochim. Biophys. Acta. 1962;59:336-346.
7. Bromer W.W., Sheehan S.K., Berns A.W., Arquilla E.R.. Preparation and properties of fluoresceinthiocarbamyl insulins. Biochemistry. 1967;6:2378-2388.
8. Riddles P.W., Blakeley R.L., Zerner B.. Reassessment of Ellman's reagent. Methods Enzymol. 1983;91:49-60.
9. Holmgren A., Reichard P.. Thioredoxin 2: Cleavage with cyanogen bromide. Eur. J. Biochem. 1967;2:187-196.
10. Schagger H., von Jagow G.. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 1987;166:368-379.
11. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J.. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;193:265-275.
12. Lakowicz, J.R., Principles of Fluorescence Spectroscopy, Plenum Press, New York, 1983, pp. 14-18.
13. Saito N., Yamanaka H., Nagasawa S.. A novel HPLC method for glutathione-insulin transhydrogenase assay using fluorescence labeled insulin. J. Vet. Med. Sci. 1991;53:1-4.