Soluble expression of human leukemia inhibitory factor with protein disulfide isomerase in Escherichia coli and its simple purification

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Jung, A Song ^a   Koo, Bon Kyung ^a   Chong, Seon Ha ^a   Kim, Kyunhoo ^b   Choi, Dong Kyu ^b   Vu, Thu Trang Thi ^a   Nguyen, Minh Tan ^a   Jeong, Boram ^a   Ryu, Han Bong ^a   Kim, Injune ^b   Jang, Yeon Jin ^a   Robinson, Robert Charles ^c,d   Choe, Han ^a  

^a University of Ulsan College of Medicine   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ENDOTOXIN; GLYCINE; HYBRID PROTEIN; LEUKEMIA INHIBITORY FACTOR; PROTEIN DISULFIDE ISOMERASE; PROTEINASE; TEV PROTEASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; EMBRYONIC STEM CELL; ESCHERICHIA COLI; LOW TEMPERATURE; MASS SPECTROMETRY; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; SOLUBILITY;

AMINO ACID SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE ORDER; HUMANS; LEUKEMIA INHIBITORY FACTOR; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN DISULFIDE-ISOMERASES; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 84892666852     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0083781     Document Type: Article

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