A simple method for production and purification of soluble and biologically active recombinant human leukemia inhibitory factor (hLIF) fusion protein in Escherichia coli

Journal of Biotechnology

Volumn 151, Issue 4, 2011, Pages 295-302

  Imsoonthornruksa, Sumeth ^a   Noisa, Parinya ^a   Parnpai, Rangsun ^a   Ketudat Cairns, Mariena ^a  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

Author keywords

Fusion protein; Histidine tag; HLIF; Recombinant protein; Solubility; Stem cells

Indexed keywords

ALKALINE PHOSPHATASE ACTIVITY; BIOLOGICAL ACTIVITIES; COST EFFECTIVE; CYTOKINES; DOSE-DEPENDENT MANNER; FUSION PROTEINS; FUSION TAG; HIGH PURITY; HISTIDINE TAG; HLIF; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LC/MS/MS; LEUKEMIA INHIBITORY FACTORS; MOUSE EMBRYONIC STEM CELLS; N-TERMINALS; PLURIPOTENCY; RECOMBINANT PROTEIN; SIMPLE METHOD; TF-1 CELLS; USER-FRIENDLY METHODS; WESTERN BLOTS;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANIMAL CELL CULTURE; CELL CULTURE; DISEASES; ESCHERICHIA COLI; MASS SPECTROMETRY; PHOSPHATASES; PURIFICATION; SOLUBILITY; STEM CELLS;

PROTEINS;

ALKALINE PHOSPHATASE; COMPLEMENTARY DNA; HEXAHISTIDINE; HYBRID PROTEIN; LEUKEMIA INHIBITORY FACTOR; OCTAMER TRANSCRIPTION FACTOR 4; PEPTIDE HISTIDINE ISOLEUCINE; RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR; RECOMBINANT PROTEIN; STAGE SPECIFIC EMBRYO ANTIGEN 1; THIOREDOXIN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CELL STRUCTURE; EMBRYONIC STEM CELL; ENZYME ACTIVITY; ESCHERICHIA COLI; FEMALE; HUMAN; HUMAN ERYTHROLEUKEMIC CELL; HUMAN TISSUE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LEUKEMIA CELL; MASS SPECTROMETRY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; WESTERN BLOTTING;

ANIMALS; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; EMBRYONIC STEM CELLS; ESCHERICHIA COLI; FEMALE; GENETIC VECTORS; HUMANS; LEUKEMIA INHIBITORY FACTOR; MICE; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION;

ESCHERICHIA COLI;

EID: 79951551289     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.12.020     Document Type: Article

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