Cloning of dextransucrase gene from Leuconostoc citreum HJ-P4 and its high-level expression in E. coli by low temperature induction

Journal of Microbiology and Biotechnology

Volumn 19, Issue 8, 2009, Pages 829-835

  Yi, Ah Rum ^a   Lee, So Ra ^a   Jang, Myoung Uoon ^a   Park, Jung Mi ^a   Eom, Hyun Ju ^a   Han, Nam Soo ^a   Kim, Tae Jip ^a  

^a Chungbuk National University   (South Korea)

Author keywords

Dextransucrase (DSase); Gene cloning; Leuconostoc citreum HJ P4; Low temperature induction; Overexpression

Indexed keywords

ACETIC ACID; CALCIUM ION; DEXTRANSUCRASE; LEUCONOSTOC DEXTRANSUCRASE; MALTOSE; SUCROSE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE OVEREXPRESSION; GENETIC CODE; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; LEUCONOSTOC; LEUCONOSTOC CITREUM; LEUCONOSTOC MESENTEROIDES; LOW TEMPERATURE PROCEDURES; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; SEQUENCE HOMOLOGY;

CALCIUM CHLORIDE; CLONING, MOLECULAR; COLD TEMPERATURE; ENZYME ACTIVATION; ENZYME INDUCTION; ESCHERICHIA COLI; GENES, BACTERIAL; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; ISOPROPYL THIOGALACTOSIDE; LEUCONOSTOC; RECOMBINANT PROTEINS;

LEUCONOSTOC; LEUCONOSTOC CITREUM;

EID: 70849088908     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0812.683     Document Type: Article

References (34)

1. Demuth, K., H. J. Jördening, and K. Buchholz. 2002. Oligosaccharide synthesis by dextransucrase: New unconventional acceptors. Carbohydr. Res. 337: 1811-1820.
2. Eom, H. J., D. M. Seo, and N. S. Han. 2007. Selection of psychrotrophic Leuconostoc spp. producing highly active dextransucrase from lactate fermented vegetables. Int. J. Food Microbiol. 117: 61-67.
3. Kang, H. K., Y. M. Kim, and D. M. Kim. 2008. Functional, genetic, and bioinformatic characterization of dextransucrase (DSRBCB4) gene in Leuconostoc mesenteroides B-1299CB4. J. Microbiol. Biotechnol. 18: 1050-1058.
4. Kim, D. and J. F. Robyt. 1995. Dextransucrase constitutive mutants of Leuconostoc mesenteroides B-1299. Enz. Microb. Technol. 17: 1050-1056.
5. Kim, J. F., H. Jeong, J. S. Lee, S. H. Choi, M. Ha, C. G. Hur, et al. 2008. Complete genome sequence of Leuconostoc citreum KM20. J. Bacteriol. 190: 3093-3094.
6. Koh, Y. H., M. D. Kim, N. S. Han, and J. H. Seo. 2002. Quantitative analysis of Leuconostoc mesenteroides and Lactobacillus plantarum populations by a competitive polymerase chain reaction. J . Microbiol. Biotechnol. 12: 801-806.
7. Korakli, M. and R. F. Vogel. 2006. Structure/function relationship of homopolysaccharide producing glycansucrase and therapeutic potential of their synthesised glycans. Appl. Microbiol. Biotechnol. 71: 790-803.
8. Kralj, S., G. H. van Geel-Schutten, M. M. Dondorff, S. Kirsanovs, M. J. van der Maarel, and L. Dijkhuizen. 2004. Glucan synthesis in the genus Lactobacillus: Isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. Microbiology 150: 3681-3690. (Pubitemid 39545315)
9. Lee, M. S., S. K. Cho, H. J. Eom, S. Y. Kim, T. J. Kim, and N. S. Han. 2008. Optimized substrate concentrations for production of long-chain isomaltooligosaccharides using dextransucrase of Leuconostoc mesenteroides B-512F. J. Microbiol. Biotechnol. 18: 1141-1145.
10. Majumder, A., R. K. Purama, and A. Goyal. 2007. An overview of purification methods of glycoside hydrolase family 70 dextransucrase. Indian J. Microbiol. 47: 197-206.
11. Makarova, K., A. Slesarev, Y. Wolf, A. Sorokin, B. Mirkin, E. Koonin, et al. 2006. Comparative genomics of the lactic acid bacteria. Proc. Natl. Acad. Sci. U.S.A. 103: 15611-15616.
12. Malten, M., R. Hollmann, W. D. Deckwer, and D. Jahn. 2005. Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol. Bioeng. 89: 206-218.
13. Miller, A. W. and J. F. Robyt. 1986. Activation and inhibition of dextransucrase by calcium. Biochim. Biophys. Acta 880: 32-39.
14. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
15. Monchois, V., M. Remaud-Simeon, P. Monsan, and R. M. Wilemot. 1998. Cloning and sequencing of a gene coding for an extracellular dextransucrase (DSRB) from Leuconostoc mesenteroides NRRL B-1299 synthesizing only a α(1-6) glucan. FEMS Microbiol. Lett. 159: 307-315.
16. Monchois, V., M. Remaud-Simeon, R. R. Russell, P. Monsan, and R. M. Willemot. 1997. Characterization of Leuconostoc mesenteroides NRRL B-512F dextransucrase (DSRS) and identification of amino-acid residues playing a key role in enzyme activity. Appl. Microbiol. Biotechnol. 48: 465-472.
17. Monchois, V., R. M. Willemot, and P. Monsan. 1999. Glucansucrases: Mechanism of action and structure-function relationship. FEMS Microbiol. Rev. 23: 131-151.
18. Moulis, C., A. Arcache, P.C. Escalier, M. Rinaudo, P. Monsan, M. Remaud-Simeon, and G. Potocki-Veronese. 2006. High-level production and purification of a fully active recombinant dextransucrase from Leuconostoc mesenteroides NRRL B-512F. FEMS Microbiol. Lett. 261: 203-210.
19. Moulis, C., G. Joucla, D. Harrison, E. Fabre, G. Ptocki-Veronese, P. Monsan, and M. Remaud-Simeon. 2006. Understanding the polymerization mechanism of glycoside-hydrolase family 70 glucansucrases. J. Biol. Chem. 281: 31254-31267.
20. Naessens, M., A. Cerdobbel, W. Soetaert, and E. J. Vandamme. 2005. Leuconostoc dextransucrase and dextran: Production, properties and applications. J. Chem. Technol. Biotechnol. 80: 845-860.
21. Neubauer, H., A. Bauche, and B. Mollet. 2003. Molecular characterization and expression analysis of the dextransucrase DsrD of Leuconostoc mesenteroides Lcc4 in homologous and heterologous Lactococcus lactis cultures. Microbiology 149: 973-982. (Pubitemid 36553366)
22. Park, J. M., N. S. Han, and T. J. Kim. 2007. Rapid detection and isolation of known and putative α-L-arabinofuranosidase genes using degenerate PCR primers. J. Microbiol. Biotechnol. 17: 481-489.
23. Remaud-Simeon, M., R. M. Willemot, P. Sarcabal, G. P. de Montalk, and P. Monsan. 2000. Glucansucrases: Molecular engineering and oligosaccharide synthesis. J. Mol. Catal. B Enz. 10: 117-128.
24. Robyt, J. F., S. H. Yoon, and R. Mukerjea. 2008. Dextransucrase and the mechanism for dextran biosynthesis. Carbohydr. Res. 343: 3039-3048.
25. Seo, D. M., S. Y. Kim, H. J. Eom, and N. S. Han. 2007. Synbiotic synthesis of oligosaccharides during milk fermentation by addition of Leuconostoc starter and sugars. J. Microbiol. Biotechnol. 17: 1758-1764.
26. Shirano, Y. and D. Shibata. 1990. Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level. FEBS Lett. 271: 128-130. (Pubitemid 20336221)
27. Swistowska, A. M., S. Wittrock, W. Collisi, and B. Hofer. 2008. Heterologous hyper-expression of a glucansucrase-type glycosyltransferase gene. Appl. Microbiol. Biotechnol. 79: 255-261.
28. Terpe, K. 2006. Overview of bacterial expression systems for heterologous protein production: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 72: 211-222.
29. Ueda, S., T. Shiroza, and H. K. Kuramitsu. 1988. Sequence analysis of the gtfC gene from Streptococcus mutans GS-5. Gene 69: 101-109. (Pubitemid 18224569)
30. van Hijum, S. A. F. T., S. Kralj, L. K. Ozimek, L. Dijkhuizen, and I. G. H. van Geel-Schutten. 2006. Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria. Microbiol. Mol. Biol. Rev. 70: 157-176.
31. Yalin, Y., L. Jin, W. Jianhua, T. Da, and T. Zigang. 2008. Expression and characterization of dextransucrase gene dsrX from Leuconostoc mesenteroides in Escherichia coli. J. Biotechnol. 133: 505-512.
32. Yang, J., C. Yang, H. Jiang, and C. Qiao. 2008. Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates. Biodegradation 19: 831-839.
33. Yang, Q., M. Li, J. Xu, Y. Bao, X. Lei, and L. An. 2003. Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli. Biotechnol. Lett. 25: 101-104.
34. Zhang, H., Y. Hu., C. Zhu., B. Zhu, and Y. Wang. 2008. Cloning, sequencing and expression of a dextransucrase gene (dexYG) from Leuconostoc mesenteroides. Biotechnol. Lett. 30: 1441-1446.