Expression and purification of biologically active human FGF2 containing the b′a′ domains of human PDI in escherichia coli

Applied Biochemistry and Biotechnology

Volumn 170, Issue 1, 2013, Pages 67-80

  Song, Jung A ^a   Koo, Bon Kyung ^a   Chong, Seon Ha ^a   Kwak, Jihye ^b   Ryu, Han Bong ^a   Nguyen, Minh Tan ^a   Vu, Thu Trang Thi ^a   Jeong, Boram ^a   Kim, Seong Who ^b   Choe, Han ^a  

^a University of Ulsan   (South Korea)

^b University of Ulsan College of Medicine   (South Korea)

Author keywords

Escherichia coli; hFGF2; PDI; Protein purification

Indexed keywords

CENTRAL NERVOUS SYSTEMS; EXPERIMENTAL PROCEDURE; FIBROBLAST GROWTH FACTOR; HFGF2; ION EXCHANGE CHROMATOGRAPHY; PDI; PROTEIN PURIFICATION; PURIFICATION PROCEDURES;

BIOACTIVITY; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; PROTEINS; STEM CELLS;

PURIFICATION;

FIBROBLAST GROWTH FACTOR 2; PROTEIN DISULFIDE ISOMERASE;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; ESCHERICHIA COLI; FIBROBLAST; HIPPOCAMPUS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR STABILITY; MOUSE; NERVE CELL; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SOLUBILITY;

AMINO ACID SEQUENCE; ANIMALS; CELLS, CULTURED; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION; HIPPOCAMPUS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NEURONS; NIH 3T3 CELLS; PLASMIDS; PROTEIN DISULFIDE-ISOMERASES; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 84876690604     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-013-0140-3     Document Type: Article

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