High-level expression and efficient one-step chromatographic purification of a soluble human leukemia inhibitory factor (LIF) in Escherichia coli

Protein Expression and Purification

Volumn 90, Issue 1, 2013, Pages 20-26

  Imaizumi, Keitaro ^a,b   Nishikawa, Shin Ichi ^a   Tarui, Hiroshi ^c   Akuta, Teruo ^a,b,d  

^a RIKEN Center for Biosystems Dynamics Research   (Japan)

^b RIKEN   (Japan)

^c RIKEN YOKOHAMA INSTITUTE   (Japan)

^d Kyokuto Pharmaceutical Industrial Co Ltd   (Japan)

Author keywords

Affinity tag based protein purification; Escherichia coli; Human leukemia inhibitor factor; Pluripotent stem cells; Soluble protein expression

Indexed keywords

LEUKEMIA INHIBITORY FACTOR; LIF PROTEIN, HUMAN; RECOMBINANT PROTEIN; STAT3 PROTEIN; STAT3 PROTEIN, MOUSE;

ANIMAL; ARTICLE; EMBRYONIC STEM CELL; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; TUMOR CELL CULTURE;

ANIMALS; EMBRYONIC STEM CELLS; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; LEUKEMIA INHIBITORY FACTOR; MICE; RECOMBINANT PROTEINS; STAT3 TRANSCRIPTION FACTOR; TUMOR CELLS, CULTURED;

EID: 84877843888     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.04.006     Document Type: Article

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