Large-scale production of soluble recombinant amyloid-β peptide 1-42 using cold-inducible expression system

Protein Expression and Purification

Volumn 86, Issue 1, 2012, Pages 53-57

  Kim, Eun Kyung ^a   Moon, Jeong Chan ^a   Lee, Jeong Mi ^a   Jeong, Min Seop ^a   Oh, Choongseob ^b   Ahn, Sung Min ^a   Yoo, Yung Joon ^b   Jang, Ho Hee ^a  

^a Gachon University   (South Korea)

^b Gwangju Institute of Science and Technology (GIST)   (South Korea)

Author keywords

Aggregation prone; Alzheimer's disease; Amyloid peptide 1 42; Cell viability; Cold induction; Soluble expression

Indexed keywords

AMYLOID BETA PROTEIN; AMYLOID BETA PROTEIN (1 42); AMYLOID BETA-PROTEIN (1-42); HYBRID PROTEIN; PEPTIDE FRAGMENT;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL SURVIVAL; COLD; ESCHERICHIA COLI; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; HUMAN; METHODOLOGY; NEUROBLASTOMA; OPEN READING FRAME; PATHOLOGY; PROTEIN DEGRADATION; SOLUBILITY; TUMOR CELL LINE;

AMINO ACID SEQUENCE; AMYLOID BETA-PEPTIDES; CELL LINE, TUMOR; CELL SURVIVAL; CHROMATOGRAPHY, AFFINITY; COLD TEMPERATURE; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; NEUROBLASTOMA; OPEN READING FRAMES; PEPTIDE FRAGMENTS; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRANSFORMATION, GENETIC;

EID: 84867892987     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.08.021     Document Type: Article

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