Direct observation of microtubule pushing by cortical dynein in living cells

Molecular Biology of the Cell

Volumn 25, Issue 1, 2014, Pages 95-106

  Mazel, Tomáš ^a   Biesemann, Anja ^a   Krejczy, Magda ^a   Nowald, Janos ^a   Müller, Olga ^a   Dehmelt, Leif ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CORTICAL DYNEIN; CYTOPLASMIC DYNEIN; DYNACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; ENHANCED GREEN FLUORESCENT PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 2; MICROTUBULE ASSOCIATED PROTEIN 2C; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BENDING; BLEACHING; CARBOXY TERMINAL SEQUENCE; CELL MOTION; CELL STRUCTURE; CONTROLLED STUDY; DIRECTIONAL MOVEMENT; DISSOCIATION; EUKARYOTIC CELL; FLUORESCENCE MICROSCOPY; INTERMEDIATE FILAMENT; KINETICS; MICROTUBULE; MICROTUBULE ASSEMBLY; MOTOR ACTIVITY; OBSERVATION; PRIORITY JOURNAL; ROTATION; STOCHASTIC MODEL; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; TURNOVER TIME;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; DYNEINS; MICE; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; SINGLE-CELL ANALYSIS; STOCHASTIC PROCESSES; TIME-LAPSE IMAGING;

EID: 84891799739     PISSN: 10591524     EISSN: 19394586     Source Type: Journal    
DOI: 10.1091/mbc.E13-07-0376     Document Type: Article

References (30)

1. Ahmad FJ, He Y, Myers KA, Hasaka TP, Francis F, Black MM, Baas PW (2006).Effects of dynactin disruption and dynein depletion on axonal microtubules. Traffic 7, 524-537.
2. Ahmad FJ, Hughey J, Wittmann T, Hyman A, Greaser M, Baas PW (2000). Motor proteins regulate force interactions between microtubules and microfilaments in the axon. Nat Cell Biol 2, 276-280.
3. Collins ES, Balchand SK, Faraci JL, Wadsworth P, Lee WL (2012). Cell cycle-regulated cortical dynein/dynactin promotes symmetric cell division by differential pole motion in anaphase. Mol Biol Cell 23, 3380-3390.
4. Dehmelt L, Bastiaens PI (2010). Spatial organization of intracellular communication: insights from imaging. Nat Rev Mol Cell Biol 11, 440-452.
5. Dehmelt L, Bastiaens P (2011). Self-organization in cells. In: Principles of Evolution, ed. H Meyer-Ortmanns and S Thurner, Heidelberg, Germany: Springer, 219-238.
6. Dehmelt L, Nalbant P, Steffen W, Halpain S (2006). A microtubule-based, dynein-dependent force induces local cell protrusions: implications for neurite initiation. Brain Cell Biol 35, 39-56.
7. Dujardin DL, Barnhart LE, Stehman SA, Gomes ER, Gundersen GG, Vallee RB (2003). A role for cytoplasmic dynein and LIS1 in directed cell movement. J Cell Biol 163, 1205-1211.
8. Dujardin DL, Vallee RB (2002). Dynein at the cortex. Curr Opin Cell Biol 14, 44-49.
9. Du Q, Macara IG (2004). Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell 119, 503-516.
10. Faire K, Waterman-Storer CM, Gruber D, Masson D, Salmon ED, Bulinski JC (1999). E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics. J Cell Sci 112, 4243-4255.
11. Felgner H, Frank R, Biernat J, Mandelkow EM, Mandelkow E, Ludin B, Matus A, Schliwa M (1997). Domains of neuronal microtubule-associated proteins and flexural rigidity of microtubules. J Cell Biol 138, 1067-1075.
12. Gennerich A, Carter AP, Reck-Peterson SL, Vale RD (2007). Force-induced bidirectional stepping of cytoplasmic dynein. Cell 131, 952-965.
13. Grabham PW, Seale GE, Bennecib M, Goldberg DJ, Vallee RB (2007). Cytoplasmic dynein and LIS1 are required for microtubule advance during growth cone remodeling and fast axonal outgrowth. J Neurosci 27, 5823-5834.
14. Gusnowski EM, Srayko M (2011). Visualization of dynein-dependent microtubule gliding at the cell cortex: implications for spindle positioning. J Cell Biol 194, 377-386.
15. Habermann A, Schroer TA, Griffiths G, Burkhardt JK (2001). Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles. J Cell Sci 114, 229-240.
16. Helfand BT, Mikami A, Vallee RB, Goldman RD (2002). A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization. J Cell Biol 157, 795-806.
17. Kiyomitsu T, Cheeseman IM (2012). Chromosome-and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol 14, 311-317.
18. Laan L, Pavin N, Husson J, Romet-Lemonne G, van Duijn M, Lopez MP, Vale RD, Julicher F, Reck-Peterson SL, Dogterom M (2012). Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters. Cell 148, 502-514.
19. Merdes A, Ramyar K, Vechio JD, Cleveland DW (1996). A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell 87, 447-458.
20. Nedelec F (2002). Computer simulations reveal motor properties generating stable antiparallel microtubule interactions. J Cell Biol 158, 1005-1015.
21. Ozer RS, Halpain S (2000). Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton. Mol Biol Cell 11, 3573-3587.
22. Roberts AJ et al. (2009). AAA+ Ring and linker swing mechanism in the dynein motor. Cell 136, 485-495.
23. Sbalzarini IF, Koumoutsakos P (2005). Feature point tracking and trajectory analysis for video imaging in cell biology. J Struct Biol 151, 182-195.
24. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22, 1567-1572.
25. Toba S, Watanabe TM, Yamaguchi-Okimoto L, Toyoshima YY, Higuchi H (2006). Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein. Proc Natl Acad Sci USA 103, 5741-5745.
26. Umeyama T, Okabe S, Kanai Y, Hirokawa N (1993). Dynamics of microtubules bundled by microtubule associated protein 2C (MAP2C). J Cell Biol 120, 451-465.
27. Vaughan KT, Tynan SH, Faulkner NE, Echeverri CJ, Vallee RB (1999). Colocalization of cytoplasmic dynein with dynactin and CLIP-170 at microtubule distal ends. J Cell Sci 112, 1437-1447.
28. Vogel SK, Pavin N, Maghelli N, Julicher F, Tolic-Norrelykke IM (2009). Self-organization of dynein motors generates meiotic nuclear oscillations. PLoS Biol 7, e1000087.
29. Watanabe N, Mitchison TJ (2002). Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295, 1083-1086.
30. Zhu J, Burakov A, Rodionov V, Mogilner A (2010). Finding the cell center by a balance of dynein and myosin pulling and microtubule pushing: a computational study. Mol Biol Cell 21, 4418-4427.