A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly

Cell

Volumn 87, Issue 3, 1996, Pages 447-458

  Merdes, Andreas ^a   Ramyar, Kasra ^b   Vechio, Janet D ^a   Cleveland, Don W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM PROTEIN; DYNEIN ADENOSINE TRIPHOSPHATASE; NUCLEAR PROTEIN; TUBULIN;

ANIMAL CELL; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; MICROTUBULE ASSEMBLY; MITOSIS SPINDLE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; XENOPUS LAEVIS;

ANIMALIA; ANURA; ASTER; XENOPUS LAEVIS;

EID: 0030298137     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)81365-3     Document Type: Article

References (43)

1. Church, G.M., and Gilbert, W. (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991-1995.
2. Compton, D.A., and Cleveland, D.W. (1993). NuMA is required for the proper completion of mitosis. J. Cell Biol. 120, 947-957.
3. Compton, D.A., and Luo, C. (1995). Mutation of the predicted p34 cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis. J. Cell Sci. 108, 621-633.
4. Compton, D.A., Yen, T.J., and Cleveland, D.W. (1991). Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds. J. Cell Biol. 112, 1083-1097.
5. Compton, D.A., Szilak, I., and Cleveland, D.W. (1992). Primary structure of NuMA, an intraouclear protein that defines a novel pathway for segregation of proteins at mitosis. J. Cell Biol. 116, 1395-1408.
6. Doxsey, S.J., Stein, P., Evans, L., Calarco, P.D., and Kirschner, M. (1994). Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell 76, 639-650.
7. Frohman, M.A., Dush, M.K., and Martin, G.R. (1988). Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002.
8. Gaglio, T., Saredi, A., and Compton, D.A. (1995). NuMA is required for the organization of microtubules into aster-like mitotic arrays. J. Cell Biol. 131, 693-708.
9. Gill, S.R., Schroer, T.A., Szilak, I., Steuer, E.R., Sheetz, M.P., and Cleveland, D.W. (1991). Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein. J. Cell Biol. 115, 1639-1650.
10. Gounari, F., Merdes, A., Quinlan, R., Hess, J., FitzGerald, P.G., Ouzounis, C.A., and Georgatos, S.D. (1993). Bovine filensin possesses primary and secondary structure similarity to intermediate filament proteins. J. Cell Biol. 121, 847-853.
11. Heald, R., Tournebize, R., Blank, T., Sandaltzopoulos, R., Becker, P., Hyman, A., and Karsenti, E. (1996). Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420-425.
12. Hyman, A.A., and Karsenti, E. (1996). Morphogenetic properties of microtubules and mitotic spindle assembly. Cell 84, 401-410.
13. Kallajoki, M., Weber, K., and Osborn, M. (1991). A 210 kDa nuclear matrix protein is a functional part of the mitotic spindle; a microinjection study using SPN monoclonal antibodies. EMBO J. 10, 3351-3362.
14. Kallajoki, M., Weber, K., and Osborn, M. (1992). Ability to organize microtubules in taxol-treated mitotic PtK2 cells goes with the SPN antigen and not with the centrosome. J. Cell Sci. 102, 91-102.
15. Kallajoki, M., Harborth, J., Weber, K., and Osborn, M. (1993). Microinjection of a monoclonal antibody against SPN antigen, now identified by peptide sequences as the NuMA protein, induces micronuclei in PtK2 cells. J. Cell Sci. 104, 139-150.
16. Koonce, M.P., Grissom, P.M., and McIntosh, J.R. (1992). Dynein from Dictyostelium: primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein. J. Cell Biol. 119, 1597-1604.
17. Lydersen, B.K., and Pettijohn, D.E. (1980). Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell. Cell 22, 489-499.
18. Madine, M.A., Khoo, C.Y., Mills, A.D., and Laskey, R.A. (1995). MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Nature 375, 421-424.
19. Maekawa, T., Leslie, R., and Kuriyama, R. (1991). Identification of a minus end-specific microtubule associated protein located at the mitotic poles in cultured mammalian cells. Eur. J. Cell Biol. 54, 255-267.
20. Mastronarde, D.N., McDonald, K.L., Ding, R., and McIntosh, J.R. (1993). Interpolar spindle microtubules in PtK cells. J. Cell Biol. 123, 1475-1489.
21. Matthies, H.J.G., McDonald, H.B., Goldstein, L.S.B., and Theurkauf, W.B. (1996). Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J. Cell Biol. 134, 455-464.
22. Mazia, D. (1987). The chromosome cycle and the centrosome cycle in the mitotic cycle. Int. Rev. Cytol. 100, 49-92.
23. McNally, F.J., Okawa, K., Iwamatsu, A., and Vale, R.D. (1996). Katanin, the microtubule-severing ATPase, is concentrated at centrosomes. J. Cell Sci. 109, 561-567.
24. Murray, A.W. (1991). Cell cycle extracts. In Methods in Cell Biology, Volume 36, B.K. Kay and H.B. Peng, eds. (San Diego, California: Academic Press), pp. 581-605.
25. Niclas, J., Allen, V.J., and Vale, R.D. (1996). Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport. J. Cell Biol. 133, 558-593.
26. Nicklas, R.B. (1989). The motor for poleward chromosome movement in anaphase is in or near the kinetochore. J. Cell Biol. 109, 2245-2255.
27. Nicklas, R.B., Lee, G.M., Rieder, C.L., and Rupp, G. (1989). Mechanically cut mitotic spindles: clean cuts and stable microtubules. J. Cell Sci. 94, 415-423.
28. Saredi, A., Howard, L., and Compton, D.A. (1996). NuMA assembles into an extensive filamentous structure when expressed in the cell cytoplasm. J. Cell Sci. 109, 619-630.
29. Sawin, K.E., and Mitchison, T.J. (1991). Mitotic spindle assembly by two different pathways in vitro. J. Cell Biol. 112, 925-940.
30. Sawin, K.E., LeGuellec, K., Philippe, M., and Mitchison, T.J. (1992a). Mitotic spindle organization by a plus-end-directed microtubule motor. Nature 359, 540-543.
31. Sawin, K.E., Mitchison, T.J., and Wordeman, L.G. (1992b). Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J. Cell Sci. 101, 303-313.
32. Schroer, T.A., and Sheetz, M.P. (1991). Two activators of microtubule-based vesicle transport. J. Cell Biol. 115, 1309-1318.
33. Shamu, C.E., and Murray, A.W. (1992). Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase. J. Cell Biol. 117, 921-934.
34. Steuer, E.R., Wordeman, L., Schroer, T.A., and Sheetz, M.P. (1990). Localization of cytoplasmic dynein to mitotic spindles and kinetochores. Nature 345, 266-268.
35. Tang, T.K., Tang, C.J.C., Chen, Y.L., and Wu, C.W. (1993). Nuclear proteins of the bovine esophageal epithelium: the NuMA gene gives rise to multiple mRNAs and gene products reactive with monoclonal antibody W1. J. Cell Sci. 104, 249-260.
36. Tang, T.K., Tang, C.J.C., Chao, Y.J., and Wu, C.W. (1994). Nuclear mitotic apparatus protein (NuMA): spindle association, nuclear targeting and differential subcellular localization of various NuMA isoforms. J. Cell Sci. 107, 1389-1402.
37. Tousson, A., Zeng, C., Brinkley, B.R., and Valdivia, M.M. (1991). Centrophilin: a novel mitotic spindle protein involved in microtubule nucleation. J. Cell Biol. 112, 427-440.
38. Vaisberg, E.A., Koonce, M.P., and McIntosh, J.R. (1993). Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123, 849-858.
39. Verde, F., Serrez, J.M., Antony, C., and Karsenti, E. (1991). Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. J. Cell Biol. 112, 1177-1187.
40. Vernos, I., and Karsenti, E. (1995). Chromosomes take the lead in spindle assembly. Trends Cell Biol. 5, 297-301.
41. Walczak, C.E., Mitchison, T.J., and Desai, A. (1996). XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84, 37-47.
42. Yang, C.H., and Snyder, M. (1992). The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus. Mol. Biol. Cell 3, 1259-1267.
43. Yang, C.H., Lambie, E.J., and Snyder, M. (1992). NuMA: an unusually long coiled-coil related protein in the mammalian nucleus. J. Cell Biol. 116, 1303-1317.