Ionizing radiation induces senescence and differentiation of human dental pulp stem cells

Folia Biologica (Czech Republic)

Volumn 59, Issue 5, 2013, Pages 188-197

  Havelek, Radim ^a,d   Soukup, T ^a   Cmielova J ^a   Seifrtova M ^a   Suchanek J ^b   Vavrova J ^c   Mokry J ^a   Muthna D ^a   Rezacova M ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF DEFENCE   (Czech Republic)

^c UNIVERSITY OF DEFENCE   (Czech Republic)

^d UNIVERSITY OF PARDUBICE   (Czech Republic)

Author keywords

Dental pulp stem cells; Dentinogenesis; Ionizing radiation; Osteogenesis; Radiation induced premature differentiation

Indexed keywords

CALCIUM; CD146 ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; BONE DEVELOPMENT; BONE MATRIX; CELL AGING; CELL DIFFERENTIATION; CONTROLLED STUDY; DENTIN; HUMAN; HUMAN CELL; IONIZING RADIATION; ODONTOBLAST; OSTEOBLAST; STEM CELL; TOOTH DEVELOPMENT; TOOTH PULP;

ANTIGENS, CD146; CELL AGING; CELL COUNT; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; DENTAL PULP; HUMANS; KINETICS; OSTEOGENESIS; RADIATION, IONIZING; STEM CELLS; TIME FACTORS;

EID: 84891417054     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Baksh, D., Yao, R., Tuan, R. S. (2007) Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells 25, 1384-1392.
2. Barry, F. P., Murphy, J. M. (2004) Mesenchymal stem cells: clinical applications and biological characterization. Int. J. Biochem. Cell Biol. 36, 568-584.
3. Campisi, J. The biology of replicative senescence. (1997) Eur. J. Cancer 33, 703-709.
4. Canman, C. E., Lim, D. S., Cimprich, K. A., Taya, Y., Tamai, K., Sakaguchi, K., Appella, E., Kastan, M. B., Siliciano, J. D. (1998) Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science 281, 1677-1679.
5. waf1/cip1 in senescence-like terminal proliferation arrest induced in human tumor cells by chemotherapeutic drugs. Oncogene 18, 4808-4818.
6. Chen, L. F., Cohen, E. E., Grandis, J. R. (2010) New strategies in head and neck cancer: understanding resistance to epidermal growth factor receptor inhibitors. Clin. Cancer Res. 16, 2489-2495.
7. Chen, M. F., Lin, C. T., Chen, W. C., Yang, C. T., Chen, C. C., Liao, S. K., Liu, J. M., Lu, C. H., Lee, K. D. (2006) The sensitivity of human mesenchymal stem cells to ionizing radiation. Int. J. Radiat. Oncol. Biol. Phys. 66, 244-253.
8. Cmielova, J., Havelek, R., Soukup, T., Jiroutova, A., Visek, B., Suchanek, J., Vavrova, J., Mokry, J., Muthna, D., Bruckova, L., Filip, S., English, D., Rezacova, M. (2012) Gamma radiation induces senescence in human adult mesenchymal stem cells from bone marrow and periodontal ligaments. Int. J. Radiat. Biol. 88, 393-404.
9. Cristofalo, V. J., Pignolo, R. J., Cianciarulo, F. L., DiPaolo, B. R., Rotenberg, M. O. (1992) Changes in gene expression during senescence in culture. Exp. Gerontol. 27, 429-432.
10. Debacq-Chainiaux, F., Erusalimsky, J. D., Campisi, J., Toussaint, O. (2009) Protocols to detect senescence-associated β-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo. Nat. Protoc. 4, 1798-1806.
11. Ferlay, J., Parkin, D. M., Steliarova-Foucher, E. (2010) Estimates of cancer incidence and mortality in Europe in 2008. Eur. J. Cancer 46, 765-781.
12. Friedenstein, A. J., Chailakhjan, R. K., Lalykina K. S. (1970) The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 4, 393-403.
13. Gronthos, S., Mankani, M., Brahim, J., Robey, P. G., Shi, S. (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc. Natl. Acad. Sci. USA 97, 13625-13630.
14. Gronthos, S., Brahim, J., Li, W., Fisher, L. W., Cherman, N., Boyde, A., DenBesten, P., Robey, P. G., Shi, S. (2002) Stem cell properties of human dental pulp stem cells. J. Dent. Res. 81, 531-535.
15. Hayflick, L., Moorhead, P. S. (1961) The serial cultivation of human diploid cell strains. Exp. Cell Res. 25, 585-621.
16. Hayflick, L. (1965) The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37, 614-636.
17. Karbanová, J., Soukup, T., Suchánek, J., Pytlík, R., Corbeil, D., Mokrý, J. (2011) Characterization of dental pulp stem cells from impacted third molars cultured in low serumcontaining medium. Cells Tissues Organs 193, 344-365.
18. Mauney, J., Olsen, B. R., Volloch, V. (2010) Matrix remodeling as stem cell recruitment event: a novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol. 29, 657-663.
19. Milyavsky, M., Gan, O. I., Trottier, M., Komosa, M., Tabach, O., Notta, F., Lechman, E., Hermans, K. G., Eppert, K., Konovalova, Z., Ornatsky, O., Domany, E., Meyn, M. S., Dick, J. E. (2010) A distinctive DNA damage response in human hematopoietic stem cells reveals an apoptosis-independent role for p53 in self-renewal. Cell Stem Cell. 7, 186-197.
20. Muthna, D., Soukup, T., Vavrova, J., Mokry, J., Cmielova, J., Visek, B., Jiroutova, A., Havelek, R., Suchanek, J., Filip, S., English, D. Rezacova, M. (2010) Irradiation of adult human dental pulp stem cells provokes activation of p53, cell cycle arrest, and senescence but not apoptosis. Stem Cells Dev. 19, 1855-1862.
21. Otmani, N. (2007) Oral and maxillofacial side effects of radiation therapy on children. J. Can. Dent. Assoc. 73, 257-261.
22. Pignolo, R. J., Martin, B. G., Horton, J. H., Kalbach, A. N., Cristofalo. V. J. (1998) The pathway of cell senescence: WI-38 cells arrest in late G1 and are unable to traverse the cell cycle from a true G0 state. Exp. Gerontol. 33, 67-80.
23. Pittenger, M. F., Marshak, D. R. (2001) Introduction: stem cell biology. In: Stem Cell Biology, eds. Marshak, D. R., Gardner, R. L., Gotlieb, D. Cold Spring Harbor Laboratory Press. New York.
24. Poncin, G., Beaulieu, A., Humblet, C., Thiry, A., Oda, K., Boniver, J., Defresne, M. P. (2012) Characterization of spontaneous bone marrow recovery after sublethal total body irradiation: importance of the osteoblastic/ adipocytic balance. PLoS One 7, 1-13.
25. INK4a enrichment and the premature senescence of normal fibroblasts. Oncogene 16, 1113-1123.
26. Russell, K. C., Phinney, D. G., Lacey, M. R., Barrilleaux, B. L., Meyertholen, K. E., O'Connor, K. C. (2010) In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells 28, 788-798.
27. Schellenberg, A., Lin, Q., Schüler, H., Koch, C. M., Joussen, S., Denecke, B., Walenda, G., Pallua, N., Suschek, C. V., Zenke, M., Wagner, W. (2011) Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks. Aging 3, 873-888.
28. Schneider, U. (2011) Modeling the risk of secondary malignancies after radiotherapy. Genes 2, 1033-1049.
29. Schönmeyr, B. H., Wong, A. K., Soares, M., Fernandez, J., Clavin, N., Mehrara, B. J. (2008) Ionizing radiation of mesenchymal stem cells results in diminution of the precursor pool and limits potential for multilineage differentiation. Plast. Reconstr. Surg. 122, 64-76.
30. Sloan, A. J., Waddington, R. J. (2009) Dental pulp stem cells: what, where, how? Int. J. Paediatr. Dent. 19, 61-70.
31. + multipotent mesenchymal stromal cells. Exp. Hematol. 36, 1035-1046.
32. Stanford, C. M., Jacobson, P. A., Eanes, E. D., Lembke, L. A., Midura, R. J. (1995) Rapidly forming apatitic mineral in an osteoblastic cell line (UMR 106-01 BSP). J. Biol. Chem. 270, 9420-9428.
33. Suchánek, J., Soukup, T., Ivančaková, R., Karbanová, J., Hubková, V., Pytlík, R., Kučerová, L. (2007) Human dental pulp stem cells-isolation and long term cultivation. Acta Medica 50, 195-201.
34. Suchanek, J., Soukup, T., Visek, B., Ivancakova, R., Kucerova, L., Mokry, J. (2009) Dental pulp stem cells and their characterization. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub. 153, 31-35.
35. Suzuki, M., Boothman, D. A. (2008) Stress-induced premature senescence (SIPS)-influence of SIPS on radiotherapy. J. Radiat. Res. 49, 105-112.
36. Téclès, O., Laurent, P., Zygouritsas, S., Burger, A. S., Camps, J., Dejou, J., About, I. (2005) Activation of human dental pulp progenitor/stem cells in response to odontoblast injury. Arch. Oral Biol. 50, 103-108.
37. Wangenheim, K. H., Peterson, H. P. (1998) Control of cell proliferation by progress in differentiation: clues to mechanisms of aging, cancer causation and therapy. J. Theor. Biol. 193, 663-678.
38. Wier, M. L., Scott, R. E. (1986) Regulation of the terminal event in cellular differentiation: biological mechanisms of the loss of proliferative potential. J. Cell Biol. 102, 1955-1964.
39. 60Co γ radiation on mesenchymal stem cells (MSCs) proliferation and differentiation. Afr. J. Microbiol. Res. 4, 2161-2168.
40. Zhang, W., Walboomers, X. F., Van Kuppevelt, T. H., Daamen, W. F., Van Damme, P. A., Bian, Z., Jansen, J. A. (2008) In vivo evaluation of human dental pulp stem cells differentiated towards multiple lineages. J. Tissue Eng. Regen. Med. 2, 117-125.