Tungsten carbide-cobalt as a nanoparticulate reference positive control in in vitro genotoxicity assays

Toxicological Sciences

Volumn 137, Issue 1, 2014, Pages 125-134

  Moche, Hélène ^a,b,c   Chevalier, Dany ^c   Barois, Nicolas ^d   Lorge, Elisabeth ^b   Claude, Nancy ^b   Nesslany, Fabrice ^a,c  

^a INSTITUT PASTEUR DE LILLE   (France)

^b INSTITUT DE RECHERCHES INTERNATIONALES SERVIER   (France)

^c UFR3S Pharmacie   (France)

^d UAR2014 Us 41 Plateformes Lilloises en Biol S BioImaging Ctr Lille   (France)

Author keywords

Comet assay; Micronucleus assay; Mouse lymphoma assay; Nanoparticle; Regulatory test

Indexed keywords

COBALT; NANOPARTICLE; TUNGSTEN DERIVATIVE; METAL NANOPARTICLE; TUNGSTEN CARBIDE;

ANIMAL CELL; ARTICLE; CELL TYPE; COMET ASSAY; CONTROLLED STUDY; CYTOKINESIS; GENETIC ANALYSIS; GENOTOXICITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; LYMPHOCYTE; LYMPHOMA; LYMPHOMA CELL LINE; MICRONUCLEUS TEST; MOUSE; NANOMEDICINE; NONHUMAN; PRIMARY CULTURE; SUSPENSION; ADULT; ANIMAL; CHEMICALLY INDUCED; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECTS; FEMALE; GENETICS; MALE; METABOLISM; MICRONUCLEUS; MUTAGEN TESTING; PATHOLOGY; STANDARD; STANDARDS; TOXICITY; TUMOR CELL LINE;

ADULT; ANIMALS; CELL LINE, TUMOR; COBALT; COMET ASSAY; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; HUMANS; LYMPHOCYTES; LYMPHOMA; MALE; METAL NANOPARTICLES; MICE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MUTAGENICITY TESTS; REFERENCE STANDARDS; TUNGSTEN COMPOUNDS;

EID: 84891303496     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kft222     Document Type: Article

References (38)

1. Afssaps (2011). Recommandations relatives à l'évaluation toxicologique des médicaments sous forme nanoparticulaire. Available at: http://ansm.sante.fr/ var/ansm_site/storage/original/application/7042aee471537987a082180039a 5bded.pdf. Accessed on December 5, 2011.
2. Anard, D., Kirsch-Volders, M., Elhajouji, A., Belpaeme, K., and Lison, D. (1997). In vitro genotoxic effects of hard metal particles assessed by alkaline single cell gel and elution assays. Carcinogenesis 18, 177-184.
3. Bastian, S., Busch, W., Kühnel, D., Springer, A., Meissner, T., Holke, R., Scholz, S., Iwe, M., Pompe, W., Gelinsky, M., et al. (2009). Toxicity of tungsten carbide and cobalt-doped tungsten carbide nanoparticles in mammalian cells in vitro. Environ. Health Perspect. 117, 530-536.
4. Clare, M. G., Lorenzon, G., Akhurst, L. C., Marzin, D., van Delft, J., Montero, R., Botta, A., Bertens, A., Cinelli, S., Thybaud, V., et al. (2006). SFTGinternational collaborative study on in vitro micronucleus test II. Using human lymphocytes. Mutat. Res. 607, 37-60.
5. De Boeck, M., Hoet, P., Lombaert, N., Nemery, B., Kirsch-Volders, M., and Lison, D. (2003a). In vivo genotoxicity of hard metal dust: Induction of micronuclei in rat type II epithelial lung cells. Carcinogenesis 24, 1793-1800.
6. De Boeck, M., Lison, D., and Kirsch-Volders, M. (1998). Evaluation of the in vitro direct and indirect genotoxic effects of cobalt compounds using the alkaline comet assay. Influence of interdonor and interexperimental variability. Carcinogenesis 19, 2021-2029.
7. De Boeck, M., Lombaert, N., De Backer, S., Finsy, R., Lison, D., and Kirsch-Volders, M. (2003b). In vitro genotoxic effects of different combinations of cobalt and metallic carbide particles. Mutagenesis 18, 177-186.
8. Di Bucchianico, S., Fabbrizi, M. R., Misra, S. K., Valsami-Jones, E., Berhanu, D., Reip, P., Bergamaschi, E., and Migliore, L. (2013). Multiple cytotoxic and genotoxic effects induced in vitro by differently shaped copper oxide nanomaterials. Mutagenesis 28, 287-299.
9. Ding, M., Kisin, E. R., Zhao, J., Bowman, L., Lu, Y., Jiang, B., Leonard, S., Vallyathan, V., Castranova, V., Murray, A. R., et al. (2009). Size-dependent effects of tungsten carbide-cobalt particles on oxygen radical production and activation of cell signaling pathways in murine epidermal cells. Toxicol. Appl. Pharmacol. 241, 260-268.
10. Doak, S. H., Griffiths, S. M., Manshian, B., Singh, N., Williams, P. M., Brown, A. P., and Jenkins, G. J. (2009). Confounding experimental considerations in nanogenotoxicology. Mutagenesis 24, 285-293.
11. Doak, S. H., Manshian, B., Jenkins, G. J., and Singh, N. (2012). In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines. Mutat. Res. 745, 104-111.
12. Donaldson, K., Poland, C. A., and Schins, R. P. (2010). Possible genotoxic mechanisms of nanoparticles: Criteria for improved test strategies. Nanotoxicology 4, 414-420.
13. Elhajouji, A., Cunha, M., and Kirsch-Volders, M. (1998). Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay. Mutagenesis 13, 193-198.
14. Fenech, M., Chang, W. P., Kirsch-Volders, M., Holland, N., Bonassi, S., and Zeiger, E.; HUman MicronNucleus project. (2003). HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 534, 65-75.
15. Gonzalez, L., Lison, D. & Kirsch-Volders, M. (2008). Genotoxicity of engineered nanomaterials: A critical review. Nanotoxicology 2, 252-273.
16. IARC (2006). Cobalt in hard metals and cobalt sulfate, gallium arsenide, indium phosphide and vanadium pentoxide. IARC Monogr. Eval. Carcinog. Risks Humans World Heal. Organ. Int. Agency Res. Cancer 86, 1-294.
17. ISO (2008). ISO/TS 27687:2008-Nanotechnologies-Terminology and definitions for nano-objects-Nanoparticle, nanofibre and nanoplate.
18. Jensen, K. A., Kembouche, Y., Christiansen, E., Jacobsen, N. R., Wallin, H., and Guiot, C. (2011). Final Protocol for Producing Suitable Manufactured Nanomaterial Exposure Media. Available at: http://www.nanogenotox.eu/ files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf. Accessed on November 30, 2011.
19. Kühnel, D., Busch, W., Meissner, T., Springer, A., Potthoff, A., Richter, V., Gelinsky, M., Scholz, S., and Schirmer, K. (2009). Agglomeration of tungsten carbide nanoparticles in exposure medium does not prevent uptake and toxicity toward a rainbow trout gill cell line. Aquat. Toxicol. 93, 91-99.
20. Kühnel, D., Scheffler, K., Wellner, P., Meißner, T., Potthoff, A., Busch, W., Springer, A., and Schirmer, K. (2012). Comparative evaluation of particle properties, formation of reactive oxygen species and genotoxic potential of tungsten carbide based nanoparticles in vitro. J. Hazard. Mater. 227-228, 418-426.
21. Kroll, A., Dierker, C., Rommel, C., Hahn, D., Wohlleben, W., Schulze-Isfort, C., Göbbert, C., Voetz, M., Hardinghaus, F., and Schnekenburger, J. (2011). Cytotoxicity screening of 23 engineered nanomaterials using a test matrix of ten cell lines and three different assays. Part. Fibre Toxicol. 8, 9.
22. Landsiedel, R., Kapp, M. D., Schulz, M., Wiench, K., and Oesch, F. (2009). Genotoxicity investigations on nanomaterials: Methods, preparation and characterization of test material, potential artifacts and limitations-many questions, some answers. Mutat. Res. 681, 241-258.
23. Lison, D., Carbonnelle, P., Mollo, L., Lauwerys, R., and Fubini, B. (1995). Physicochemical mechanism of the interaction between cobalt metal and carbide particles to generate toxic activated oxygen species. Chem. Res. Toxicol. 8, 600-606.
24. Magdolenova, Z., Collins, A., Kumar, A., Dhawan, A., Stone, V., and Dusinska, M. (2013). Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology Advance Access published March 20, 2013. doi:10.3109/17435390.2013.773464
25. Magdolenova, Z., Lorenzo, Y., Collins, A., and Dusinska, M. (2012). Can standard genotoxicity tests be applied to nanoparticles? J. Toxicol. Environ. Health. A 75, 800-806.
26. Moore, M. M., Honma, M., Clements, J., Bolcsfoldi, G., Burlinson, B., Cifone, M., Clarke, J., Delongchamp, R., Durward, R., Fellows, M., et al. (2006). Mouse lymphoma thymidine kinase gene mutation assay: Follow-up meeting of the International Workshop on Genotoxicity Testing-Aberdeen, Scotland, 2003-Assay acceptance criteria, positive controls, and data evaluation. Environ. Mol. Mutagen. 47, 1-5.
27. Moulin, J. J., Wild, P., Romazini, S., Lasfargues, G., Peltier, A., Bozec, C., Deguerry, P., Pellet, F., and Perdrix, A. (1998). Lung cancer risk in hardmetal workers. Am. J. Epidemiol. 148, 241-248.
28. Muller, J., Decordier, I., Hoet, P. H., Lombaert, N., Thomassen, L., Huaux, F., Lison, D., and Kirsch-Volders, M. (2008). Clastogenic and aneugenic effects of multi-wall carbon nanotubes in epithelial cells. Carcinogenesis 29, 427-433.
29. OECD (1997). Test No. 476: In Vitro Mammalian Cell Gene Mutation Test, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing. doi:10.1787/9789264071322-en
30. OECD (2010). Test No. 487: In Vitro Mammalian Cell Micronucleus Test, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing. doi:10.1787/9789264091016-en
31. Oliver, J., Meunier, J. R., Awogi, T., Elhajouji, A., Ouldelhkim, M. C., Bichet, N., Thybaud, V., Lorenzon, G., Marzin, D., and Lorge, E. (2006). SFTG international collaborative study on in vitro micronucleus test V. Using L5178Y cells. Mutat. Res. 607, 125-152.
32. Pfuhler, S., Fellows, M., van Benthem, J., Corvi, R., Curren, R., Dearfield, K., Fowler, P., Frötschl, R., Elhajouji, A., Le Hégarat, L., et al. (2011). In vitro genotoxicity test approaches with better predictivity: Summary of an IWGT workshop. Mutat. Res. 723, 101-107.
33. Singh, N., Manshian, B., Jenkins, G. J., Griffiths, S. M., Williams, P. M., Maffeis, T. G., Wright, C. J., and Doak, S. H. (2009). NanoGenotoxicology: The DNA damaging potential of engineered nanomaterials. Biomaterials 30, 3891-3914.
34. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191.
35. Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C., and Sasaki, Y. F. (2000). Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35, 206-221.
36. Van Goethem, F., Lison, D., and Kirsch-Volders, M. (1997). Comparative evaluation of the in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: Genotoxic effects of cobalt powder, tungsten carbide and cobalt-tungsten carbide. Mutat. Res. 392, 31-43.
37. Warheit, D. B., and Donner, E. M. (2010). Rationale of genotoxicity testing of nanomaterials: Regulatory requirements and appropriateness of available OECD test guidelines. Nanotoxicology 4, 409-413.
38. Wild, P., Perdrix, A., Romazini, S., Moulin, J. J., and Pellet, F. (2000). Lung cancer mortality in a site producing hard metals. Occup. Environ. Med. 57, 568-573.