SFTG international collaborative study on in vitro micronucleus test. II. Using human lymphocytes

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 607, Issue 1, 2006, Pages 37-60

  Clare, M Gillian ^a   Lorenzon, Giocondo ^b   Akhurst, Leslie C ^c   Marzin, Daniel ^d   van Delft, Joost ^e   Montero, Regina ^f   Botta, Alain ^g   Bertens, Arma ^h   Cinelli, Serena ⁱ   Thybaud, Véronique ^j   Lorge, Elisabeth ^k  

^a Safety Assessment   (United Kingdom)

^b Prostrakan Pharmaceuticals   (France)

^c HUNTINGDON LIFE SCIENCES   (United Kingdom)

^d INSTITUT PASTEUR DE LILLE   (France)

^e TNO   (Netherlands)

^f UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^g AIX MARSEILLE UNIVERSITY   (France)

^h Notox BV   (Netherlands)

ⁱ Research Toxicology Center   (Italy)

^j SANOFI   (France)

^k INSTITUT DE RECHERCHES INTERNATIONALES SERVIER   (France)

more..

Author keywords

Aneugen; Clastogen; Cytochalasin B; Human lymphocytes; In vitro micronucleus assay

Indexed keywords

ANEUGEN; BLEOMYCIN; CLOFIBRATE; COLCHICINE; CYTARABINE; CYTOCHALASIN B; DIETHYLSTILBESTROL; FLUOROURACIL; MANNITOL; MITOMYCIN C; TIABENDAZOLE; URETHAN;

ADULT; ARTICLE; CELL CULTURE; CELL CYCLE; CELL DIVISION; CELL NUCLEUS; CHEMICAL ANALYSIS; CONTROLLED STUDY; DATA ANALYSIS; FEMALE; HUMAN; HUMAN CELL; LYMPHOCYTE; MALE; MICRONUCLEUS TEST; MITOGENICITY; MONONUCLEAR CELL; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

ADULT; ANEUGENS; BLEOMYCIN; CLOFIBRATE; COLCHICINE; CYTARABINE; DIETHYLSTILBESTROL; FEMALE; FLUOROURACIL; HUMANS; INTERNATIONAL COOPERATION; LYMPHOCYTES; MALE; MANNITOL; MICRONUCLEUS TESTS; MUTAGENS; THIABENDAZOLE; URETHANE;

EID: 33745954144     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2006.04.001     Document Type: Article

References (71)

1. Fenech M., and Morley A.A. Measurement of micronuclei in lymphocytes. Mutat. Res. 147 (1985) 29-36
2. Van Hummelen P., and Kirsch-Volders M. An improved method for the in vitro micronucleus test using human lymphocytes. Mutagenesis 5 (1990) 203-204
3. Van Hummelen P., and Kirsch-Volders M. Analysis of eight known or suspected aneugens by the in vitro human lymphocyte micronucleus test. Mutagenesis 7 (1992) 447-455
4. Fenech M. The cytokinesis block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat. Res. 285 (1993) 35-44
5. Van Hummelen P., Severi M., Pauwels W., Roosels D., Veulemans H., and Kirsch-Volders M. Cytogenetic analysis of lymphocytes from fiberglass-reinforced plastics workers occupationally exposed to styrene. Mutat. Res. 310 (1994) 157-165
6. Buchet J.P., Ferreira Jr. M., Burrion J.B., Leroy T., Kirsch-Volders M., Van Hummelen P., Jacques J., Cupers L., Delavignette J.P., and Lauwerys R. Tumor markers in serum, polyamines and modified nucleosides in urine, and cytogenetic aberrations in lymphocytes of workers exposed to polycyclic aromatic hydrocarbons. Am. J. Ind. Med. 27 (1995) 523-543
7. Thierens H., Vral A., De Ridder L., Touil N., Kirsch-Volders M., Lambert V., and Laurent C. Inter-laboratory comparison of cytogenetic endpoints for the biomonitoring of radiological workers. Int. J. Radiat. Biol. 75 (1999) 23-34
8. Norppa H., Luomahaara S., Heikanen H., Roth S., Sorsa M., Renzi L., and Lindholm C. Micronucleus assay in lymphocytes as a tool to biomonitor human exposure to aneuploidogens and clastogens. Environ. Health Perspect. 101 Suppl. 3 (1993) 139-143
9. Streffer C., Mueller W.U., Kryscio A., and Boecker W. Micronuclei-biological indicator for retrospective dosimetry after exposure to ionizing radiation. Mutat. Res. 404 (1998) 101-105
10. Mueller W.U., Nuesse M., Miller B.M., Slavotinek A., Viaggi S., and Streffer C. Micronuclei: a biological indicator of radiation damage. Mutat. Res. 366 (1996) 163-169
11. Eastmond D.A., and Pinkel D. Detection of aneuploidy and aneuploidy-inducing agents in human lymphocytes using fluorescence in situ hybridization with chromosome-specific DNA probes. Mutat. Res. 234 (1990) 303-318
12. Migliore L., and Nieri M. Evaluation of twelve potential aneuploidogenic chemicals by the in vitro human lymphocyte micronucleus assay. Toxicol. In Vitro 5 (1991) 325-336
13. Surralles J., Antoccia A., Creus A., Degrassi F., Peris F., Tanzarella C., Xamena N., and Marcos R. The effect of cytochalasin-B concentration on the frequency of micronuclei induced by four standard mutagens. Results from two laboratories. Mutagenesis 9 (1994) 347-353
14. Van Hummelen P., Elhajouji A., and Kirsch-Volders M. Clastogenic and aneugenic effects of three benzimidazole derivatives in the in vitro micronucleus test using human lymphocytes. Mutagenesis 10 (1995) 23-29
15. Parry J.M., Parry E.M., Bourner R., Doherty A., Ellard S., O'Donovan J., Hoebee B., de Stoppelaar J.M., Mohn G.R., Onfelt A., Renglin A., Schultz N., Sooderpalm-Berndes C., Jensen K.G., Kirsch-Volders M., Elhajouji A., Van Hummelen P., Degrassi F., Antoccia A., Cimini D., Izzo M., Tanzarella C., Adler I.-D., Kliesch U., Hess P., et al. The detection and evaluation of aneugenic chemicals. Mutat. Res. 353 (1996) 11-46
16. Kirsch-Volders M., and Parry E.M. Genetic toxicology of mitotic spindle inhibitors used as anticancer drugs. Mutat. Res. 355 (1996) 103-128
17. Elhajouji A., Cunha M., and Kirsch-Volders M. Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay. Mutagenesis 13 (1998) 193-198
18. Verdoodt B., Decordier I., Geleyns K., Cunha M., Cundari E., and Kirsch-Volders M. Induction of polyploidy and apoptosis after exposure to high concentrations of the spindle poison nocodazole. Mutagenesis 14 (1999) 513-520
19. Lynch A.M., and Parry J.M. The cytochalasin-B micronucleus/kinetochore assay in vitro: studies with 10 suspected aneugens. Mutat. Res. 287 (1993) 71-86
20. Channarayappa, Ong T., and Nath J. Cytogenetic effects of vincristine sulfate and ethylene dibromide in human peripheral lymphocytes: micronucleus analysis. Environ. Mol. Mutagen. 20 (1992) 117-126
21. Luomahaara S., and Norppa H. Induction of micronuclei in cultured human lymphocytes treated with vinblastine before and after mitogen stimulation. Mutat. Res. 324 (1994) 29-34
22. Gustavino B., Degrassi F., Filipponi R., Modesti D., Tanzarella C., and Rizzoni M. Mitotic indirect non-disjunction in phytohemagglutinin stimulated human lymphocytes. Mutagenesis 9 (1994) 17-21
23. Van Hummelen P., Deleener A., Vanparys P., and Kirsch-Volders M. Discrimination of aneuploidogens from clastogens by C-banding, DNA and area measurements of micronuclei from mouse bone marrow. Mutat. Res. 271 (1992) 13-28
24. Zijno A., Marcon F., Leopardi P., and Crebelli R. Simultaneous detection of X-chromosome loss and non-disjunction in cytokinesis-blocked human lymphocytes by in situ hybridization with a centromeric DNA probe; implications for the human lymphocyte in vitro micronucleus assay using cytochalasin B. Mutagenesis 9 (1994) 225-232
25. Zijno A., Leopardi P., Marcon F., and Crebelli R. Analysis of chromosome segregation by means of fluorescence in situ hybridization: application to cytokinesis-blocked human lymphocytes. Mutat. Res. 372 (1996) 211-219
26. Surralles J., Falck G., and Norppa H. In vivo cytogenetic damage revealed by FISH analysis of micronuclei in uncultured human T lymphocytes. Cytogenet. Cell Genet. 75 (1996) 151-154
27. Caria H., Chaveca T., Laires A., and Rueff J. Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes. Mutat. Res. 343 (1995) 85-94
28. Surralles J., Catalan J., Creus A., Norppa H., Xamena N., and Marcos R. Micronuclei induced by alachlor, mitomycin C and vinblastine in human lymphocytes: presence of centromeres and kinetochores and influence of staining technique. Mutagenesis 10 (1995) 417-423
29. Darroudi F., Meijers C.M., Hadjidekova V., and Natarajan A.T. Detection of aneugenic and clastogenic potential of X-rays, directly and indirectly acting chemicals in human hepatoma (Hep G2) and peripheral blood lymphocytes, using the micronucleus assay and fluorescent in situ hybridization with a DNA centromeric probe. Mutagenesis 11 (1996) 425-433
30. Huber R., Salassidis K., Kulka U., Braselmann H., and Bauchinger M. Detection of centromeres in vinblastine and radiation-induced micronuclei of human lymphocytes using FISH with an alpha satellite pancentromeric DNA probe. Environ. Mol. Mutagen. 27 (1996) 105-109
31. Schuler M., Rupa D.S., and Eastmond D.A. A critical evaluation of centromeric labeling to distinguish micronuclei induced by chromosomal loss and breakage in vitro. Mutat. Res. 392 (1997) 81-95
32. Elhajouji A., Van Hummelen P., and Kirsch-Volders M. Indications for a threshold of chemically induced aneuploidy in vitro in human lymphocytes. Environ. Mol. Mutagen. 26 (1995) 292-304
33. A. Elhajouji, M. Kirsch-Volders. Combination of flow sorting of micronuclei mn with fish for aneuploidy threshold evaluation, in: Proceedings of the 25th Annual Meeting of the European Environmental Mutagen Society, Noordwijkerhout, Netherlands, 18-23 June 1995, Mutat. Res. 360 (1996) 251.
34. Kirsch-Volders M., Elhajouji A., Cundari E., and Van Hummelen P. The in vitro micronucleus test: a multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat. Res. 392 (1997) 19-30
35. Elhajouji A., Tibaldi F., and Kirsch-Volders M. Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes. Mutagenesis 12 (1997) 133-140
36. Castelain P., Van Hummelen P., Deleener A., and Kirsch-Volders M. Automated detection of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis 8 (1993) 285-293
37. Van Hummelen P., Nousse M., Castelain P., and Kirsch-Volders M. Aneugen-induced micronuclei (MN) in human lymphocytes may be discerned using image analysis techniques when cell-cycle stage is taken into account. Environ. Mol. Mutagen. 25 (1995) 269-278
38. Frieauff W., Pootter-Locher F., Cordier A., and Suter W. Automatic analysis of the in vitro micronucleus test on V79 cells. Mutat. Res. 413 (1998) 57-68
39. Boecker W., Mueller W.U., and Streffer C. Image processing algorithms for the automated micronucleus assay in binucleated human lymphocytes. Cytometry 19 (1995) 283-294
40. Vral A., Verhaegen F., Thierens H., and De Ridder L. The in vitro cytokinesis-block micronucleus assay: a detailed description of an improved slide preparation technique for the automated detection of micronuclei in human lymphocytes. Mutagenesis 9 (1994) 439-443
41. Verhaegen F., Vral A., Seuntjens J., Schipper N.W., De Ridder L., and Thierens H. Scoring of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes by automated image analysis. Cytometry 17 (1994) 119-127
42. Thierens H., Vral A., De Scheerder F., De Ridder L., and Tates A. Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation. Int. J. Radiat. Biol. 72 (1997) 319-324
43. Elhajouji A., Santos A.P., Van Hummelen P., and Kirsch-Volders M. Metabolic differences between whole blood and isolated lymphocyte cultures for micronucleus (MN) induction by cyclophosphamide and benzo[a]pyrene. Mutagenesis 9 (1994) 307-313
44. Vian L., Van Hummelen P., Bichet N., Gouy D., and Kirsch-Volders M. Evaluation of hydroquinone and chloral hydrate on the in vitro micronucleus test on isolated lymphocytes. Mutat. Res. 334 (1995) 1-7
45. Migliore L., Nieri M., Amodio S., and Loprieno N. The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures. Mutat. Res. 227 (1989) 167-172
46. Ellard S., and Parry E.M. A modified protocol for the cytochalasin B in vitro micronucleus assay using whole human blood or separated lymphocyte cultures. Mutagenesis 8 (1993) 317-320
47. Surralles J., Xamena N., Creus A., Catalan J., Norppa H., and Marcos R. Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures. Mutat. Res. 341 (1995) 169-184
48. Vian L., Bichet N., and Gouy D. The in vitro micronucleus test on isolated human lymphocytes. Mutat. Res. 291 (1993) 93-102
49. O'Donovan M.R., Johns S., and Wilcox P. The effect of PHA stimulation on lymphocyte sub-populations in whole-blood cultures. Mutagenesis 10 (1995) 371-374
50. Fenech M., and Morley A.A. Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo aging and low dose X-irradiation. Mutat. Res. 161 (1986) 193-198
51. Ramalho A., Sunjevaric I., and Natarajan A.T. Use of the frequencies of micronuclei as quantitative indicators of X-ray-induced chromosomal aberrations in human peripheral blood lymphocytes: comparison of two methods. Mutat. Res. 207 (1988) 141-146
52. E. Lorge, V. Thybaud, M. Aardema, J. Oliver, A. Wakata, G. Lorenzon, D. Marzin, SFTG International Collaborative Study on in vitro Micronucleus Test. Part I. General conditions of the study, submitted, 2004.
53. Fenech M., and Morley A.A. The effect of donor age on spontaneous and induced micronuclei. Mutat. Res. 148 (1985) 99-105
54. Gantenberg H.W., Wuttke K., Streffer C., and Muller W.U. Micronuclei in human lymphocytes irradiated in vitro or in vivo. Radiat. Res. 128 (1991) 276-281
55. Erexson G.L., Kligerman A.D., Bryant M.F., Sontag M.R., and Halperin E.C. Induction of micronuclei by X-radiation in human, mouse and rat peripheral blood lymphocytes. Mutat. Res. 253 (1991) 193-198
56. Fenech M.F., Dunaiski V., Osborne Y., and Morley A.A. The cytokinesis-block micronucleus assay as a biological dosimeter in spleen and peripheral blood lymphocytes of the mouse following acute whole-body irradiation. Mutat. Res. 263 (1991) 119-126
57. Ganguly B.B. Cell division, chromosomal damage and micronucleus formation in peripheral lymphocytes of healthy donors: related to donor's age. Mutat. Res. 295 (1993) 135-148
58. Gaziev A.I., Sologub G.R., Fomenko L.A., Zaichkina S.I., Kosyakova N.I., and Bradbury R.J. Effect of vitamin-antioxidant micronutrients on the frequency of spontaneous and in vitro gamma-ray-induced micronuclei in lymphocytes of donors: the age factor. Carcinogenesis 17 (1996) 493-499
59. Fenech M.F., Rinaldi J., and Surralles J. The origin of micronuclei induced by cytosine arabinoside and its synergistic interaction with hydroxyurea in human lymphocytes. Mutagenesis 9 (1994) 273-277
60. Mavournin K.H., Blakey D.H., Cimino M.C., Salamone M.F., and Heddle J.A. The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutat. Res. 239 (1990) 29-80
61. Adler I.D., Kliesch U., Van Hummelen P., and Kirsch-Volders M. Mouse micronucleus tests with known and suspect spindle poisons - results from two laboratories. Mutagenesis 6 (1991) 47-53
62. von der Hude W., Kalweit S., Engelhardt G., McKiernan S., Kasper P., Slacik-Erben R., Miltenburger H.G., Honarvar N., Fahrig R., Görlitz B., Albertini S., Kirschner S., Utesch D., Pötter-Locher F., Stopper H., and Madle S. In vitro micronucleus assay with Chinese hamster V79 cells - results of a collaborative study with in situ exposure to 26 chemical substances. Mutat. Res. 468 (2000) 137-163
63. Miller B., Pötter-Locher F., Seelbach A., Stopper H., Utesch D., and Madle S. Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosomal aberration assay: position of the GUM working group on the in vitro micronucleus test. Mutat. Res. 410 (1998) 81-116
64. Ashby J. Genotoxicity data supporting the proposed metabolic activation of ethyl carbamate (urethane) to a carcinogen: the problem now posed by methyl carbamate. Mutat. Res. 260 (1991) 307-308
65. NTP technical report on toxicity studies in drinking water and urethane in 5% ethanol administered to F344/N rats and B6C3F1 mice, Toxic Rep. Ser. No. 52, 1996, pp. 1-91.
66. Ishidate M., Harnois M.C., and Sofuni T. A comparative analysis of data on the clastogenicity of 951 chemical substances in mammalian cell cultures. Mutat. Res. 195 (1988) 151-213
67. Fenech M. The in vitro micronucleus technique. Mutat. Res. 455 (2000) 81-95
68. Marshall R.R., Murphy M., Kirkland D.J., and Bentley K.S. Fluorescence in situ hybridization (FISH) with chromosome-specific centromere probes: a sensitive method to detect aneuploidy. Mutat. Res. 372 (1996) 233-245
69. Fenech, et al. Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project. Mutat. Res. 534 (2003) 45-64
70. Rosefort C., Fauth E., and Zankl H. Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay. Mutagenesis 19 (2004) 277-284
71. Ishidate M., Sofuni T., and Yoshikawa K. Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens. Gann Monogr. Cancer Res. 27 (1981) 95-108