Fluorescence linked enzyme chemoproteomic strategy for discovery of a potent and selective DAPK1 and ZIPK inhibitor

ACS Chemical Biology

Volumn 8, Issue 12, 2013, Pages 2715-2723

  Carlson, David A ^a   Franke, Aaron S ^b   Weitzel, Douglas H ^a   Speer, Brittany L ^a   Hughes, Philip F ^a   Hagerty, Laura ^a   Fortner, Christopher N ^a   Veal, James M ^c   Barta, Thomas E ^a   Zieba, Bartosz J ^b   Somlyo, Avril V ^b   Sutherland, Cindy ^d   Deng, Jing Ti ^d   Walsh, Michael P ^d   Macdonald, Justin A ^d   Haystead, Timothy A J ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c Quanticel Pharmaceuticals   (United States)

^d LIBIN CARDIOVASCULAR INSTITUTE OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

1 (3 CHLOROPHENYL) 6 [(2 HYDROXYETHYL)THIO] 1,5 DIHYDRO 4H PYRAZOLO[3,4 D]PYRIMIDIN 4 ONE; 2 [[1 (3 CHLOROPHENYL) 4 OXO 4,5 DIHYDRO 1H PYRAZOLO[3,4 D]PYRIMIDIN 6 YL]THIO]PROPANAMIDE; ADENOSINE TRIPHOSPHATE; CALCIUM ION; CALYCULIN A; DEATH ASSOCIATED PROTEIN KINASE; DEATH ASSOCIATED PROTEIN KINASE 1; DEATH ASSOCIATED PROTEIN KINASE 1 INHIBITOR; GREEN FLUORESCENT PROTEIN; HS 38; HS 43; MOLECULAR SCAFFOLD; MYOSIN LIGHT CHAIN; MYOSIN LIGHT CHAIN PHOSPHATASE; PROTEIN SERINE THREONINE KINASE INHIBITOR; PYRIMIDINONE DERIVATIVE; REGULATORY MYOSIN LIGHT CHAIN; UNCLASSIFIED DRUG; ZIPPER INTERACTING PROTEIN KINASE; ZIPPER INTERACTING PROTEIN KINASE INHIBITOR;

ANIMAL CELL; ANIMAL TISSUE; AORTA; ARTERY MUSCLE; ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG MECHANISM; DRUG POTENCY; DRUG PROTEIN BINDING; DRUG SCREENING; DRUG SELECTIVITY; DRUG STRUCTURE; DRUG TARGETING; ENZYMATIC ASSAY; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; EX VIVO STUDY; FLUORESCENCE ANALYSIS; FLUORESCENCE LINKED ENZYME CHEMOPROTEOMIC STRATEGY; HUMAN; HUMAN CELL; IC 50; ILEUM; MOUSE; MUSCLE EXCITATION; MUSCLE STRENGTH; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; RABBIT; SMOOTH MUSCLE CONTRACTILITY;

ADENOSINE TRIPHOSPHATE; ANIMALS; AORTA; BINDING, COMPETITIVE; CALCIUM; DEATH-ASSOCIATED PROTEIN KINASES; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; ILEUM; MICE; MUSCLE CONTRACTION; MUSCLE, SMOOTH; MYOCYTES, SMOOTH MUSCLE; MYOSIN-LIGHT-CHAIN KINASE; PHOSPHORYLATION; PRIMARY CELL CULTURE; PROTEIN KINASE INHIBITORS; PROTEOMICS; PYRAZOLES; PYRIMIDINONES; RABBITS; RECOMBINANT FUSION PROTEINS;

EID: 84890817125     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb400407c     Document Type: Article

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