Visualizing of signaling proteins on endosomes utilizing knockdown and reconstitution approach

Methods in Enzymology

Volumn 534, Issue , 2014, Pages 47-63

  Jeoung, Myoungkun ^a   Galperin, Emilia ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Endocytosis; ERK1 2; Fluorescence microscopy; MEK1 2; siRNA

Indexed keywords

DNA; PROTEIN; SHORT HAIRPIN RNA;

ARTICLE; CELL LINE; CONTROLLED STUDY; ENDOSOME; HUMAN; HUMAN CELL; KNOCKDOWN AND RECONSTITUTION; LENTIVIRINAE; PRIORITY JOURNAL; PROCEDURES; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

LENTIVIRUS;

ENDOCYTOSIS; ERK1/2; FLUORESCENCE MICROSCOPY; MEK1/2; SIRNA;

ENDOSOMES; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LENTIVIRUS; MAP KINASE KINASE 2; MICROSCOPY, FLUORESCENCE; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION;

EID: 84890686014     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-397926-1.00003-2     Document Type: Chapter

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