Three-chromophore fret microscopy to analyze multiprotein interactions in living cells

Nature Methods

Volumn 1, Issue 3, 2004, Pages 209-217

  Galperin, Emilia ^a   Verkhusha, Vladislav V ^a   Sorkin, Alexander ^a  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CELL STRAIN COS1; CERCOPITHECUS; COMPUTER ASSISTED DIAGNOSIS; EVALUATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION REGULATION; METHODOLOGY; MULTIPHOTON MICROSCOPY; PHYSIOLOGY; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION REGULATION; IMAGE INTERPRETATION, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; PROTEIN INTERACTION MAPPING; SIGNAL TRANSDUCTION;

EID: 15744403602     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth720     Document Type: Article

References (32)

1. Gaits, F. & Hahn, K. Shedding light on cell signaling: Interpretation of FRET biosensors. Sci. STKE 2003, PE3 (2003).
2. Jares-Erijman, E.A. & Jovin, T.M. FRET imaging. Nat. Biotechnol. 21, 1387–1395 (2003).
3. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906–918 (2002).
4. Chiu, V.K. et al. Ras signalling on the endoplasmic reticulum and the Golgi. Nat. Cell Biol. 4, 343–350 (2002).
5. Haj, F.G., Verveer, P.J., Squire, A., Neel, B.G. & Bastiaens, P.I. Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. Science 295, 1708–1711 (2002).
6. Jiang, X. & Sorkin, A. Coordinated traffic of Grb2 and Ras during epidermal growth factor receptor endocytosis visualized in living cells. Mol. Biol. Cell 13, 1522–1535 (2002).
7. Sorkin, A., McClure, M., Huang, F. & Carter, R. Interaction of EGF receptor and grb2 in living cells visualized by fluorescence resonance energy transfer (FRET) microscopy. Curr. Biol. 10, 1395–1398 (2000).
8. Zhang, J., Ma, Y., Taylor, S.S. & Tsien, R.Y. Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc. Natl. Acad. Sci. USA 98, 14997–15002 (2001).
9. Mochizuki, N. et al. Spatio-temporal images of growth-factor-induced activation of Ras and Rap1. Nature 411, 1065–1068 (2001).
10. Violin, J.D., Zhang, J., Tsien, R.Y. & Newton, A.C. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J. Cell Biol. 161, 899–909 (2003).
11. Ohba, Y., Kurokawa, K. & Matsuda, M. Mechanism of the spatio-temporal regulation of Ras and Rapl. EMBO J. 22, 859–869 (2003).
12. Kraynov, V.S. et al. Localized Rac activation dynamics visualized in living cells. Science 290, 333–337 (2000).
13. Matz, M.V. et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969–973 (1999).
14. Labas, Y.A. et al. Diversity and evolution of the green fluorescent protein family. Proc. Natl. Acad. Sci. USA 99, 4256–4261 (2002).
15. Gurskaya, N.G. et al. GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 507, 16–20 (2001).
16. Verkhusha, V.V. & Lukyanov, K.A. The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nat. Biotechnol. 22, 289–296 (2004).
17. Campbell, R.E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 7877–7882 (2002).
18. Wouters, F.S., Verveer, P.J. & Bastiaens, P.I. Imaging biochemistry inside cells. Trends Cell Biol. 11, 203–211 (2001).
19. Watrob, H.M., Pan, C.P. & Barkley, M.D. Two-step FRET as a structural tool. J. Am. Chem. Soc. 125, 7336–7343 (2003).
20. Ohya, Y., Yabuki, K., Hashimoto, M., Nakajima, A. & Ouchi, T. Multistep fluorescence resonance energy transfer in sequential chromophore array constructed on oligo-DNA assemblies. Bioconjug. Chem. 14, 1057–1066 (2003).
21. Liu, J. & Lu, Y. FRET study of a trifluorophore-labeled DNAzyme. J. Am. Chem. Soc. 124, 15208–15216 (2002).
22. Pollok, B.A. & Heim, R. Using GFP in FRET-based applications. Trends Cell Biol. 9, 57–60 (1999).
23. Gordon, G.W., Berry, G., Liang, X.H., Levine, B. & Herman, B. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74, 2702–2713 (1998).
24. Wouters, F.S., Bastiaens, P.I., Wirtz, K.W. & Jovin, T.M. FRET microscopy demonstrates molecular association of non-specific lipid transfer protein (nsLTP) with fatty acid oxidation enzymes in peroxisomes. EMBO J. 17, 7179–7189 (1998).
25. De Renzis, S., Sonnichsen, B. & Zerial, M. Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes. Nat. Cell Biol.4, 124–133 (2002).
26. Galperin, E. & Sorkin, A. Visualization of Rab5 activity in living cells by FRET microscopy and influence of plasma-membrane-targeted Rab5 on clathrin–dependent endocytosis. J. Cell Sci. 116, 4799–4810 (2003).
27. Carter, R.E. & Sorkin, A. Endocytosis of functional epidermal growth factor receptor-green fluorescent protein chimera. J. Biol. Chem. 273, 35000–35007(1998).
28. Jiang, X. & Sorkin, A. Epidermal growth factor receptor internalization through clathrin–coated pits requires Cbl RING finger and proline-rich domains but not receptor polyubiquitylation. Traffic 4, 529–543 (2003).
29. Jiang, X., Huang, F., Marusyk, A. & Sorkin, A. Grb2 regulates internalization of EGF receptors through clathrin-coated pits. Mol. Biol. Cell 14, 858–870(2003).
30. Kohl, T., Heinze, K.G., Kuhlemann, R., Koltermann, A. & Schwille, P. A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins. Proc. Natl. Acad. Sci. USA 99, 12161–12166(2002).
31. Xia, Z. & Liu, Y. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81, 2395–2402 (2001).
32. Berney, C. & Danuser, G. FRET or no FRET: A quantitative comparison. Biophys. J. 84, 3992–4010 (2003).