Monitoring CHO cell cultures: Cell stress and early apoptosis assessment by mass spectrometry

Journal of Biotechnology

Volumn 168, Issue 4, 2013, Pages 452-461

  Schwamb, Sebastian ^a,b   Munteanu, Bogdan ^a,b   Meyer, Björn ^a,b   Hopf, Carsten ^a,b   Hafner, Mathias ^a,b   Wiedemann, Philipp ^a,b  

^a Center for Applied Research in Applied Biomedical Mass Spectrometry (ABIMAS)   (Germany)

^b MANNHEIM UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

Apoptosis; Cell culture; MALDI TOF MS; Monitoring; Viability

Indexed keywords

CELLULAR VIABILITY; CHINESE HAMSTER OVARY; CLASSIFICATION MODELS; MALDI TOF MS; MALDI-MASS SPECTROMETRY; PROCESS DEVELOPMENT; SIGNAL INTENSITIES; VIABILITY;

CELL CULTURE; MASS SPECTROMETRY; MONITORING;

CELL DEATH;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE MONITORING; CELL STRESS; CELL VIABILITY; CHO CELL; CLASSIFICATION; CONTROLLED STUDY; INTACT CELL MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL;

CRICETULUS GRISEUS; MAMMALIA;

APOPTOSIS; CELL CULTURE; MALDI TOF MS; MONITORING; VIABILITY;

ANIMALS; APOPTOSIS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84888853071     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.10.014     Document Type: Article

References (38)

1. Aebersold R., Mann M. Mass spectrometry-based proteomics. Nature 2003, 422:198-207.
2. Al-Rubeai M., Fussenegger M. Monitoring of apoptosis. Cell Engineering 2004, 281-306. Kluwer Academic Publishers. M. Al-Rubeai, M. Fussenegger (Eds.).
3. Baker K.N., Rendall M.H., Patel A., Boyd P., Hoare M., Freedman R.B., James D.C. Rapid monitoring of recombinant protein products: a comparison of current technologies. Trends in Biotechnology 2002, 20:149-156.
4. Benjamini Y., Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society: Series B 1995, 57:289-300.
5. Carvell J.P., Dowd J.E. On-line measurements and control of viable cell density in cell culture manufacturing processes using radio-frequency impedance. Cytotechnology 2006, 50:35-48.
6. Clarke C., Doolan P., Barron N., Meleady P., O'Sullivan F., Gammell P., Melville M., Leonard M., Clynes M. Predicting cell-specific productivity from CHO gene expression. Journal of Biotechnology 2011, 151:159-165.
7. Cotter T.G., Al-Rubeai M. Cell death (apoptosis) in cell culture systems. Trends in Biotechnology 1995, 13:150-155.
8. De Jesus M., Wurm F.M. Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors. European Journal of Pharmaceutics and Biopharmaceutics: Official Journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2011, 78:184-188.
9. Dong H., Shen W., Cheung M.T., Liang Y., Cheung H.Y., Allmaier G., Kin-Chung Au O., Lam Y.W. Rapid detection of apoptosis in mammalian cells by using intact cell MALDI mass spectrometry. Analyst 2011, 136:5181-5189.
10. Feng H.T., Wong N.S., Sim L.C., Wati L., Ho Y., Lee M.M. Rapid characterization of high/low producer CHO cells using matrix-assisted laser desorption/ionization time-of-flight. Rapid Communications in Mass Spectrometry 2010, 24:1226-1230.
11. Fenselau C., Demirev P.A. Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrometry Reviews 2001, 20:157-171.
12. Galluzzi L., Maiuri M.C., Vitale I., Zischka H., Castedo M., Zitvogel L., Kroemer G. Cell death modalities: classification and pathophysiological implications. Cell Death and Differentiation 2007, 14:1237-1243.
13. Griffin T.J., Seth G., Xie H., Bandhakavi S., Hu W.S. Advancing mammalian cell culture engineering using genome-scale technologies. Trends in Biotechnology 2007, 25:401-408.
14. Guerrera I.C., Kleiner O. Application of mass spectrometry in proteomics. Bioscience Reports 2005, 25:71-93.
15. Hacker D.L., De Jesus M., Wurm F.M. 25 years of recombinant proteins from reactor-grown cells - where do we go from here?. Biotechnology Advances 2009, 27:1023-1027.
16. Karas M., Hillenkamp F. Laser desorption ionization of proteins with molecular masses exceeding 10,000Da. Analytical Chemistry 1988, 60:2299-2301.
17. Kondragunta B., Joshi B.H., Han J., Brorson K.A., Puri R.K., Moreira A.R., Rao G. Bioreactor environment-sensitive sentinel genes as novel metrics for cell culture scale-down comparability. Biotechnology Progress 2012, 28:1138-1151.
18. Kroemer G., Galluzzi L., Vandenabeele P., Abrams J., Alnemri E.S., Baehrecke E.H., Blagosklonny M.V., El-Deiry W.S., Golstein P., Green D.R., Hengartner M., Knight R.A., Kumar S., Lipton S.A., Malorni W., Nunez G., Peter M.E., Tschopp J., Yuan J., Piacentini M., Zhivotovsky B., Melino G. Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death and Differentiation 2009, 16:3-11.
19. Krysko D.V., Vanden Berghe T., D'Herde K., Vandenabeele P. Apoptosis and necrosis: detection, discrimination and phagocytosis. Methods 2008, 44:205-221.
20. Kumar N., Maurya P., Gammell P., Dowling P., Clynes M., Meleady P. Proteomic profiling of secreted proteins from CHO cells using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. Biotechnology Progress 2008, 24:273-278.
21. Marks D.M. Equipment design considerations for large scale cell culture. Cytotechnology 2003, 42:21-33.
22. Matasci M., Hacker D.L., Baldi L., Wurm F.M. Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects. Drug Discovery Today: Technologies 2009, 5:e37-e42.
23. Munteanu B., von Reitzenstein C., Hansch G.M., Meyer B., Hopf C. Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping. Analytical and Bioanalytical Chemistry 2012, 404:2277-2286.
24. Nienow A.W. Reactor engineering in large scale animal cell culture. Cytotechnology 2006, 50:9-33.
25. Read E.K., Park J.T., Shah R.B., Riley B.S., Brorson K.A., Rathore A.S. Process analytical technology (PAT) for biopharmaceutical products: part I. Concepts and applications. Biotechnology and Bioengineering 2010, 105:276-284.
26. Seth G., Hossler P., Yee J.C., Hu W.S. Engineering cells for cell culture bioprocessing - physiological fundamentals. Advances in Biochemical Engineering/Biotechnology 2006, 101:119-164.
27. Singh R.P., Al-Rubeai M., Gregory C.D., Emery A.N. Cell death in bioreactors: a role for apoptosis. Biotechnology and Bioengineering 1994, 44:720-726.
28. Teixeira A.P., Oliveira R., Alves P.M., Carrondo M.J. Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative. Biotechnology Advances 2009, 27:726-732.
29. Walsh G. Biopharmaceutical benchmarks 2010. Nature Biotechnology 2010, 28:917-924.
30. Wang M.J., Liu S., Liu Y., Zheng D. Actinomycin D enhances TRAIL-induced caspase-dependent and -independent apoptosis in SH-SY5Y neuroblastoma cells. Neuroscience Research 2007, 59:40-46.
31. Wei M.C., Zong W.X., Cheng E.H., Lindsten T., Panoutsakopoulou V., Ross A.J., Roth K.A., MacGregor G.R., Thompson C.B., Korsmeyer S.J. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 2001, 292:727-730.
32. Welker M. Proteomics for routine identification of microorganisms. Proteomics 2011, 11:3143-3153.
33. Wiedemann P., Guez J.S., Wiegemann H.B., Egner F., Quintana J.C., Asanza-Maldonado D., Filipaki M., Wilkesman J., Schwiebert C., Cassar J.P., Dhulster P., Suhr H. In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states. Biotechnology and Bioengineering 2011, 108:2884-2893.
34. Woolley J.F., Al-Rubeai M. The application of SELDI-TOF mass spectrometry to mammalian cell culture. Biotechnology Advances 2009, 27:177-184.
35. Woolley J.F., Al-Rubeai M. The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnology and Bioengineering 2009, 104:590-600.
36. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 2004, 22:1393-1398.
37. Wurstle M.L., Laussmann M.A., Rehm M. The central role of initiator caspase-9 in apoptosis signal transduction and the regulation of its activation and activity on the apoptosome. Experimental Cell Research 2012, 318:1213-1220.
38. Zhang X., Scalf M., Berggren T.W., Westphall M.S., Smith L.M. Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry. Journal of the American Society for Mass Spectrometry 2006, 17:490-499.