Iterative saturation mutagenesis of -6 subsite residues in cyclodextrin glycosyltransferase from Paenibacillus macerans To improve maltodextrin specificity for 2-O-D-glucopyranosyl-L-ascorbic acid synthesis

Applied and Environmental Microbiology

Volumn 79, Issue 24, 2013, Pages 7562-7568

  Han, Ruizhi ^a,c   Liu, Long ^a,c   Shin, Hyun Dong ^b   Chen, Rachel R ^b   Li, Jianghua ^a,c   Du, Guocheng ^a,c   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

^c Synergetic Innovation Center of Food Safety and Nutrition   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC REACTIONS; CYCLIZATION ACTIVITY; CYCLODEXTRIN GLYCOSYLTRANSFERASE; DISPROPORTIONATIONS; PAENIBACILLUS MACERANS; PROTEIN ENGINEERING; SATURATION MUTAGENESIS; SUBSTRATE SPECIFICITY;

CATALYSIS; CYCLIZATION; CYCLODEXTRINS; MUTAGENESIS;

ASCORBIC ACID;

CATALYSIS; ENZYME ACTIVITY; GENETIC ENGINEERING; MICROBIAL ACTIVITY; MUTAGENICITY; PROTEIN; REACTION KINETICS; SATURATION; SUBSTRATE;

2 O (BETA D GLUCOPYRANOSYL)ASCORBIC ACID; 2-O-(BETA-D-GLUCOPYRANOSYL)ASCORBIC ACID; ASCORBIC ACID; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; DRUG DERIVATIVE; GLUCOSYLTRANSFERASE; MALTODEXTRIN; MUTANT PROTEIN; POLYSACCHARIDE;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLIC ENGINEERING; METABOLISM; METHODOLOGY; MISSENSE MUTATION; PAENIBACILLUS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

ASCORBIC ACID; BINDING SITES; GLUCOSYLTRANSFERASES; KINETICS; METABOLIC ENGINEERING; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION, MISSENSE; PAENIBACILLUS; POLYSACCHARIDES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 84888222854     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02918-13     Document Type: Article

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