Site-saturation engineering of lysine 47 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance substrate specificity towards maltodextrin for enzymatic synthesis of 2-O-d-glucopyranosyl-l-ascorbic acid (AA-2G)

Applied Microbiology and Biotechnology

Volumn 97, Issue 13, 2013, Pages 5851-5860

  Han, Ruizhi ^a   Liu, Long ^a   Shin, Hyun Dong ^b   Chen, Rachel R ^b   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

2 O Glucopyranosyl l ascorbic acid (AA 2G); Cyclodextrin glycosyltransferase (CGTase); l ascorbic acid (l AA); Maltodextrin; Site saturation engineering

Indexed keywords

2-O-GLUCOPYRANOSYL-L-ASCORBIC ACID (AA-2G); CYCLIZATION ACTIVITY; CYCLODEXTRIN GLYCOSYLTRANSFERASE; HYDROGEN BONDING INTERACTIONS; L-ASCORBIC ACID; MALTODEXTRINS; PAENIBACILLUS MACERANS; SUBSTRATE SPECIFICITY;

AMINO ACIDS; CYCLIZATION; HYDROGEN BONDS; POLYSACCHARIDES; REACTION KINETICS; SUBSTRATES;

ASCORBIC ACID;

2 O D GLUCOPYRANOSYL L ASCORBIC ACID; ASCORBIC ACID DERIVATIVE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; LYSINE; MALTODEXTRIN; PHENYLALANINE; UNCLASSIFIED DRUG;

ASCORBIC ACID; BACTERIOLOGY; BACTERIUM; BIOENGINEERING; CHEMICAL BONDING; ENZYME ACTIVITY; POLYSACCHARIDE; SUBSTRATE;

ARTICLE; CYCLIZATION; DRUG STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME SPECIFICITY; NONHUMAN; PAENIBACILLUS; PAENIBACILLUS MACERANS; PH; PLASMID; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SUBSTITUTION; ASCORBIC ACID; ENZYME STABILITY; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; KINETICS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PAENIBACILLUS; POLYSACCHARIDES; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; TEMPERATURE;

PAENIBACILLUS MACERANS;

EID: 84879414928     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4514-1     Document Type: Article

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